Nitrogen depletion blocks growth stimulation driven by the expression of nitric oxide synthase in tobacco

Nitric oxide (NO) is a messenger molecule widespread studied in plant physiology. Latter evidence supports the lack of a NO-producing system involving a NO synthase (NOS) activity in higher plants. However, a NOS gene from the unicellular marine alga Ostreococcus tauri (OtNOS) was characterized in r...

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Autores principales: Nejamkin, Andrés, Foresi, Noelia, Mayta, Martín L., Lodeyro, Anabella F., Del Castello, Fiorella, Correa-Aragunde, Natalia, Carrillo, Néstor, Lamattina, Lorenzo
Formato: article artículo publishedVersion
Lenguaje:Inglés
Publicado: Frontiers Media 2020
Materias:
Nitric oxide
Nitric oxide synthase
Nicotiana tabacum
Nitrogen
Plant growth
Seed production
Tobacco
Acceso en línea:http://hdl.handle.net/2133/18754
http://hdl.handle.net/2133/18754
Aporte de:
Repositorio Hipermedial de la Universidad Nacional de Rosario (UNR) de Universidad Nacional de Rosario
id I15-R121-2133-18754
record_format dspace
institution Universidad Nacional de Rosario
institution_str I-15
repository_str R-121
collection Repositorio Hipermedial de la Universidad Nacional de Rosario (UNR)
language Inglés
orig_language_str_mv eng
topic Nitric oxide
Nitric oxide synthase
Nicotiana tabacum
Nitrogen
Plant growth
Seed production
Tobacco
spellingShingle Nitric oxide
Nitric oxide synthase
Nicotiana tabacum
Nitrogen
Plant growth
Seed production
Tobacco
Nejamkin, Andrés
Foresi, Noelia
Mayta, Martín L.
Lodeyro, Anabella F.
Del Castello, Fiorella
Correa-Aragunde, Natalia
Carrillo, Néstor
Lamattina, Lorenzo
Nitrogen depletion blocks growth stimulation driven by the expression of nitric oxide synthase in tobacco
topic_facet Nitric oxide
Nitric oxide synthase
Nicotiana tabacum
Nitrogen
Plant growth
Seed production
Tobacco
description Nitric oxide (NO) is a messenger molecule widespread studied in plant physiology. Latter evidence supports the lack of a NO-producing system involving a NO synthase (NOS) activity in higher plants. However, a NOS gene from the unicellular marine alga Ostreococcus tauri (OtNOS) was characterized in recent years. OtNOS is a genuine NOS, with similar spectroscopic fingerprints to mammalian NOSs and high NO producing capacity. We are interested in investigating whether OtNOS activity alters nitrogen metabolism and nitrogen availability, thus improving growth promotion conditions in tobacco. Tobacco plants were transformed with OtNOS under the constitutive CaMV 35S promoter. Transgenic tobacco plants expressing OtNOS accumulated higher NO levels compared to siblings transformed with the empty vector, and displayed accelerated growth in different media containing sufficient nitrogen availability. Under conditions of nitrogen scarcity, the growth promoting effect of the OtNOS expression is diluted in terms of total leaf area, protein content and seed production. It is proposed that OtNOS might possess a plant growth promoting effect through facilitating N remobilization and nitrate assimilation with potential to improve crop plants performance.
format article
artículo
publishedVersion
author Nejamkin, Andrés
Foresi, Noelia
Mayta, Martín L.
Lodeyro, Anabella F.
Del Castello, Fiorella
Correa-Aragunde, Natalia
Carrillo, Néstor
Lamattina, Lorenzo
author_facet Nejamkin, Andrés
Foresi, Noelia
Mayta, Martín L.
Lodeyro, Anabella F.
Del Castello, Fiorella
Correa-Aragunde, Natalia
Carrillo, Néstor
Lamattina, Lorenzo
author_sort Nejamkin, Andrés
title Nitrogen depletion blocks growth stimulation driven by the expression of nitric oxide synthase in tobacco
title_short Nitrogen depletion blocks growth stimulation driven by the expression of nitric oxide synthase in tobacco
title_full Nitrogen depletion blocks growth stimulation driven by the expression of nitric oxide synthase in tobacco
title_fullStr Nitrogen depletion blocks growth stimulation driven by the expression of nitric oxide synthase in tobacco
title_full_unstemmed Nitrogen depletion blocks growth stimulation driven by the expression of nitric oxide synthase in tobacco
title_sort nitrogen depletion blocks growth stimulation driven by the expression of nitric oxide synthase in tobacco
publisher Frontiers Media
publishDate 2020
url http://hdl.handle.net/2133/18754
http://hdl.handle.net/2133/18754
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