Photosynthetic characterization of flavodoxin-expressing tobacco plants reveals a high light acclimation-like phenotype

Flavodoxins are electron carrier flavoproteins present in bacteria and photosynthetic microorganisms which duplicate the functional properties of iron-sulphur containing ferredoxins and replace them under adverse environmental situations that lead to ferredoxin decline. When expressed in plant chlor...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Gómez, Rodrigo Lionel, Figueroa, Nicolás, Melzer, Michael, Hajirezaei, Mohammad-Reza, Carrillo, Néstor, Lodeyro, Anabella F.
Formato:	article artículo acceptedVersion
Lenguaje:	Inglés
Publicado:	Elsevier 2020
Materias:	Photosynthetic electron transport chain Plastoquinone pool Redox state Chloroplast High irradiation Flavodoxin
Acceso en línea:	http://hdl.handle.net/2133/18607 http://hdl.handle.net/2133/18607
Aporte de:	Repositorio Hipermedial de la Universidad Nacional de Rosario (UNR) de Universidad Nacional de Rosario

id	I15-R121-2133-18607
record_format	dspace
institution	Universidad Nacional de Rosario
institution_str	I-15
repository_str	R-121
collection	Repositorio Hipermedial de la Universidad Nacional de Rosario (UNR)
language	Inglés
orig_language_str_mv	eng
topic	Photosynthetic electron transport chain Plastoquinone pool Redox state Chloroplast High irradiation Flavodoxin
spellingShingle	Photosynthetic electron transport chain Plastoquinone pool Redox state Chloroplast High irradiation Flavodoxin Gómez, Rodrigo Lionel Figueroa, Nicolás Melzer, Michael Hajirezaei, Mohammad-Reza Carrillo, Néstor Lodeyro, Anabella F. Photosynthetic characterization of flavodoxin-expressing tobacco plants reveals a high light acclimation-like phenotype
topic_facet	Photosynthetic electron transport chain Plastoquinone pool Redox state Chloroplast High irradiation Flavodoxin
description	Flavodoxins are electron carrier flavoproteins present in bacteria and photosynthetic microorganisms which duplicate the functional properties of iron-sulphur containing ferredoxins and replace them under adverse environmental situations that lead to ferredoxin decline. When expressed in plant chloroplasts, flavodoxin complemented ferredoxin deficiency and improved tolerance to multiple sources of biotic, abiotic and xenobiotic stress. Analysis of flavodoxin-expressing plants grown under normal conditions, in which the two carriers are present, revealed phenotypic effects unrelated to ferredoxin replacement. Flavodoxin thus provided a tool to alter the chloroplast redox poise in a customized way and to investigate its consequences on plant physiology and development. We describe herein the effects exerted by the flavoprotein on the function of the photosynthetic machinery. Pigment analysis revealed significant increases in chlorophyll a, carotenoids and chlorophyll a/b ratio in flavodoxin-expressing tobacco lines. Results suggest smaller antenna size in these plants, supported by lower relative contents of light-harvesting complex proteins. Chlorophyll a fluorescence and P700 spectroscopy measurements indicated that transgenic plants displayed higher quantum yields for both photosystems, a more oxidized plastoquinone pool under steady-state conditions and faster plastoquinone dark oxidation after a pulse of saturating light. Many of these effects resemble the phenotypes exhibited by leaves adapted to high irradiation, a most common environmental hardship faced by plants growing in the field. The results suggest that flavodoxin-expressing plants would be better prepared to cope with this adverse situation, and concur with earlier observations reporting that hundreds of stress-responsive genes were induced in the absence of stress in these lines.
format	article artículo acceptedVersion
author	Gómez, Rodrigo Lionel Figueroa, Nicolás Melzer, Michael Hajirezaei, Mohammad-Reza Carrillo, Néstor Lodeyro, Anabella F.
author_facet	Gómez, Rodrigo Lionel Figueroa, Nicolás Melzer, Michael Hajirezaei, Mohammad-Reza Carrillo, Néstor Lodeyro, Anabella F.
author_sort	Gómez, Rodrigo Lionel
title	Photosynthetic characterization of flavodoxin-expressing tobacco plants reveals a high light acclimation-like phenotype
title_short	Photosynthetic characterization of flavodoxin-expressing tobacco plants reveals a high light acclimation-like phenotype
title_full	Photosynthetic characterization of flavodoxin-expressing tobacco plants reveals a high light acclimation-like phenotype
title_fullStr	Photosynthetic characterization of flavodoxin-expressing tobacco plants reveals a high light acclimation-like phenotype
title_full_unstemmed	Photosynthetic characterization of flavodoxin-expressing tobacco plants reveals a high light acclimation-like phenotype
title_sort	photosynthetic characterization of flavodoxin-expressing tobacco plants reveals a high light acclimation-like phenotype
publisher	Elsevier
publishDate	2020
url	http://hdl.handle.net/2133/18607 http://hdl.handle.net/2133/18607
work_keys_str_mv	AT gomezrodrigolionel photosyntheticcharacterizationofflavodoxinexpressingtobaccoplantsrevealsahighlightacclimationlikephenotype AT figueroanicolas photosyntheticcharacterizationofflavodoxinexpressingtobaccoplantsrevealsahighlightacclimationlikephenotype AT melzermichael photosyntheticcharacterizationofflavodoxinexpressingtobaccoplantsrevealsahighlightacclimationlikephenotype AT hajirezaeimohammadreza photosyntheticcharacterizationofflavodoxinexpressingtobaccoplantsrevealsahighlightacclimationlikephenotype AT carrillonestor photosyntheticcharacterizationofflavodoxinexpressingtobaccoplantsrevealsahighlightacclimationlikephenotype AT lodeyroanabellaf photosyntheticcharacterizationofflavodoxinexpressingtobaccoplantsrevealsahighlightacclimationlikephenotype
bdutipo_str	Repositorios
_version_	1764820410569850884

Photosynthetic characterization of flavodoxin-expressing tobacco plants reveals a high light acclimation-like phenotype

Ejemplares similares