Study of the red blood cell aggregation by coherent anti-Stokes Raman spectroscopy

In this work, we studied the nature of the molecular bonds involved in the red blood cell aggregation process by the coherent anti-Stokes Raman spectroscopy technique. Images were acquired with a commercial Leica TCS SP8 CARS confocal microscope (Leica Microsystems GmbH, Wetzlar, Germany) temporally...

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Detalles Bibliográficos
Autores principales: Toderi, Martín A., Galizzi, Gustavo E., Riquelme, Bibiana Doris, Dumas, Dominique
Otros Autores: Society of Photo-optical Instrumentation Engineers (SPIE)
Formato: conferenceObject documento de conferencia publishedVersion
Lenguaje:Inglés
Publicado: Natan T. Shaked y Oliver Hayden 2020
Materias:
Erythrocyte Aggregation
Coherent anti-Stokes Raman Spectroscopy
Dextran
Acceso en línea:http://hdl.handle.net/2133/17698
http://hdl.handle.net/2133/17698
Aporte de:
Repositorio Hipermedial de la Universidad Nacional de Rosario (UNR) de Universidad Nacional de Rosario
id I15-R121-2133-17698
record_format dspace
institution Universidad Nacional de Rosario
institution_str I-15
repository_str R-121
collection Repositorio Hipermedial de la Universidad Nacional de Rosario (UNR)
language Inglés
orig_language_str_mv eng
topic Erythrocyte Aggregation
Coherent anti-Stokes Raman Spectroscopy
Dextran
spellingShingle Erythrocyte Aggregation
Coherent anti-Stokes Raman Spectroscopy
Dextran
Toderi, Martín A.
Galizzi, Gustavo E.
Riquelme, Bibiana Doris
Dumas, Dominique
Study of the red blood cell aggregation by coherent anti-Stokes Raman spectroscopy
topic_facet Erythrocyte Aggregation
Coherent anti-Stokes Raman Spectroscopy
Dextran
description In this work, we studied the nature of the molecular bonds involved in the red blood cell aggregation process by the coherent anti-Stokes Raman spectroscopy technique. Images were acquired with a commercial Leica TCS SP8 CARS confocal microscope (Leica Microsystems GmbH, Wetzlar, Germany) temporally and spatially overlapping the pulses of two sources in the focal plane of the microscope. A pump wavelength of 810 nm to 817 nm was used for the CARS mode simultaneously with the Stokes beam at 1064 nm to excite the vibratory resonance of the symmetric hydrocarbon bonds in the lipids and that of the bonds in amino acids of the proteins. The Raman shift was also observed at the 1200 cm−1 range to study possible variations in the sialic acid on the cell membrane produced by concentrations of dextran 500 in the suspension medium. Curves of lifetime emission distribution were obtained for untreated erythrocytes and treated erythrocytes with dextran 500, particularly at a pump wavelength of 904 nm.
author2 Society of Photo-optical Instrumentation Engineers (SPIE)
author_facet Society of Photo-optical Instrumentation Engineers (SPIE)
Toderi, Martín A.
Galizzi, Gustavo E.
Riquelme, Bibiana Doris
Dumas, Dominique
format conferenceObject
documento de conferencia
publishedVersion
author Toderi, Martín A.
Galizzi, Gustavo E.
Riquelme, Bibiana Doris
Dumas, Dominique
author_sort Toderi, Martín A.
title Study of the red blood cell aggregation by coherent anti-Stokes Raman spectroscopy
title_short Study of the red blood cell aggregation by coherent anti-Stokes Raman spectroscopy
title_full Study of the red blood cell aggregation by coherent anti-Stokes Raman spectroscopy
title_fullStr Study of the red blood cell aggregation by coherent anti-Stokes Raman spectroscopy
title_full_unstemmed Study of the red blood cell aggregation by coherent anti-Stokes Raman spectroscopy
title_sort study of the red blood cell aggregation by coherent anti-stokes raman spectroscopy
publisher Natan T. Shaked y Oliver Hayden
publishDate 2020
url http://hdl.handle.net/2133/17698
http://hdl.handle.net/2133/17698
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AT galizzigustavoe studyoftheredbloodcellaggregationbycoherentantistokesramanspectroscopy
AT riquelmebibianadoris studyoftheredbloodcellaggregationbycoherentantistokesramanspectroscopy
AT dumasdominique studyoftheredbloodcellaggregationbycoherentantistokesramanspectroscopy
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