Mycobacteriophage CRB2 defines a new subcluster in mycobacteriophage classification

Mycobacteriophages are viruses -mostly temperates- that infect Mycobacterium smegmatis and sometimes Mycobacterium tuberculosis. Mycobacteriophages are grouped in clusters on the basis of the overall nucleotide sequence homology, being further divided in subclusters as more mycobacteriophage genomes...

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Autores principales: Suarez, Cristian Alejandro, Franceschelli, Jorgelina Judith, Morbidoni, Héctor Ricardo
Formato: article artículo publishedVersion
Lenguaje:Inglés
Publicado: PLOS 2019
Materias:
Bacteriophages
Genome analysis
Comparative genomics
Viral genomics
Genomic databases
Sequence alignment
Sequence databases
Acceso en línea:http://hdl.handle.net/2133/14224
http://hdl.handle.net/2133/14224
Aporte de:
Repositorio Hipermedial de la Universidad Nacional de Rosario (UNR) de Universidad Nacional de Rosario
id I15-R121-2133-14224
record_format dspace
institution Universidad Nacional de Rosario
institution_str I-15
repository_str R-121
collection Repositorio Hipermedial de la Universidad Nacional de Rosario (UNR)
language Inglés
orig_language_str_mv eng
topic Bacteriophages
Genome analysis
Comparative genomics
Viral genomics
Genomic databases
Sequence alignment
Sequence databases
spellingShingle Bacteriophages
Genome analysis
Comparative genomics
Viral genomics
Genomic databases
Sequence alignment
Sequence databases
Suarez, Cristian Alejandro
Franceschelli, Jorgelina Judith
Morbidoni, Héctor Ricardo
Mycobacteriophage CRB2 defines a new subcluster in mycobacteriophage classification
topic_facet Bacteriophages
Genome analysis
Comparative genomics
Viral genomics
Genomic databases
Sequence alignment
Sequence databases
description Mycobacteriophages are viruses -mostly temperates- that infect Mycobacterium smegmatis and sometimes Mycobacterium tuberculosis. Mycobacteriophages are grouped in clusters on the basis of the overall nucleotide sequence homology, being further divided in subclusters as more mycobacteriophage genomes are sequenced and annotated. As part of our on-going screening for novel isolates, we herein report the bioinformatics analysis of CRB2, a mycobacteriophage belonging into the Siphoviridae family that propagates at 30°C. CRB2 has a 72,217 bp genome with a 69.78% GC content that belongs to Cluster B; nucleotide comparison with other B cluster members positions CRB2 as the sole member of a new subcluster, B9, being mycobacteriophage Saguaro (belonging into subcluster B7) its closest relative. Sequencing and annotation of 14 mycobacteriophages isolated by our group has yielded six cluster A members, a singleton, four of the five members of subcluster B6, one of the three reported members of subcluster G4, and CRB2 which defines subcluster B9. Considering the massive mycobacteriophage search performed in USA and the relatively rarity of our phages, we propose that factors other than size of the sampling determine the variability of mycobacteriophage distribution, and thus a world-wide concerted mining would most likely bring extremely rare and yet undiscovered mycobacteriophages.
format article
artículo
publishedVersion
author Suarez, Cristian Alejandro
Franceschelli, Jorgelina Judith
Morbidoni, Héctor Ricardo
author_facet Suarez, Cristian Alejandro
Franceschelli, Jorgelina Judith
Morbidoni, Héctor Ricardo
author_sort Suarez, Cristian Alejandro
title Mycobacteriophage CRB2 defines a new subcluster in mycobacteriophage classification
title_short Mycobacteriophage CRB2 defines a new subcluster in mycobacteriophage classification
title_full Mycobacteriophage CRB2 defines a new subcluster in mycobacteriophage classification
title_fullStr Mycobacteriophage CRB2 defines a new subcluster in mycobacteriophage classification
title_full_unstemmed Mycobacteriophage CRB2 defines a new subcluster in mycobacteriophage classification
title_sort mycobacteriophage crb2 defines a new subcluster in mycobacteriophage classification
publisher PLOS
publishDate 2019
url http://hdl.handle.net/2133/14224
http://hdl.handle.net/2133/14224
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AT franceschellijorgelinajudith mycobacteriophagecrb2definesanewsubclusterinmycobacteriophageclassification
AT morbidonihectorricardo mycobacteriophagecrb2definesanewsubclusterinmycobacteriophageclassification
bdutipo_str Repositorios
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