Sequence characterization, in silico mapping and cytosine methylation analysis of markers linked to apospory in Paspalum notatum

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In previous studies we reported the identification of several AFLP, RAPD and RFLP molecular markers linked to apospory in Paspalum notatum . The objective of this work was to sequence these markers, obtain their flanking re -gions by chromosome walking and perform an in silico mapping analysi...

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Detalles Bibliográficos
Autores principales: Podio, Maricel, Rodríguez, María Pía, Felitti, Silvina, Stein, Juliana, Martínez, Erci Javier, Siena, Lorena Adelina, Quarin, Camilo Luis, Pessino, Silvina Claudia, Ortiz, Juan Pablo Amelio
Formato: article artículo publishedVersion
Lenguaje:Inglés
Publicado: Sociedade Brasileira de Genética 2018
Materias:
apomixis
chromosome walking
gene mapping
molecular markers
Acceso en línea:http://hdl.handle.net/2133/13785
http://hdl.handle.net/2133/13785
Aporte de:
Repositorio Hipermedial de la Universidad Nacional de Rosario (UNR) de Universidad Nacional de Rosario
id I15-R121-2133-13785
record_format dspace
institution Universidad Nacional de Rosario
institution_str I-15
repository_str R-121
collection Repositorio Hipermedial de la Universidad Nacional de Rosario (UNR)
language Inglés
orig_language_str_mv eng
topic apomixis
chromosome walking
gene mapping
molecular markers
spellingShingle apomixis
chromosome walking
gene mapping
molecular markers
Podio, Maricel
Rodríguez, María Pía
Felitti, Silvina
Stein, Juliana
Martínez, Erci Javier
Siena, Lorena Adelina
Quarin, Camilo Luis
Pessino, Silvina Claudia
Ortiz, Juan Pablo Amelio
Sequence characterization, in silico mapping and cytosine methylation analysis of markers linked to apospory in Paspalum notatum
topic_facet apomixis
chromosome walking
gene mapping
molecular markers
description In previous studies we reported the identification of several AFLP, RAPD and RFLP molecular markers linked to apospory in Paspalum notatum . The objective of this work was to sequence these markers, obtain their flanking re -gions by chromosome walking and perform an in silico mapping analysis in rice and maize. The methylation status of two apospory-related sequences was also assessed using methylation-sensitive RFLP experiments. Fourteen mo -lecular markers were analyzed and several protein-coding sequences were identified. Copy number estimates and RFLP linkage analysis showed that the sequence PnMAI3 displayed 2-4 copies per genome and linkage to apospory. Extension of this marker by chromosome walking revealed an additional protein-coding sequence map- ping in silico in the apospory-syntenic regions of rice and maize. Approximately 5 kb corresponding to different mark- ers were characterized through the global sequencing procedure. A more refined analysis based on sequence information indicated synteny with segments of chromosomes 2 and 12 of rice and chromosomes 3 and 5 of maize. Two loci associated with apomixis locus were tested in methylation-sensitive RFLP experiments using genomic DNA extracted from leaves. Although both target sequences were methylated no methylation polymorphisms associated with the mode of reproduction were detected.
format article
artículo
publishedVersion
author Podio, Maricel
Rodríguez, María Pía
Felitti, Silvina
Stein, Juliana
Martínez, Erci Javier
Siena, Lorena Adelina
Quarin, Camilo Luis
Pessino, Silvina Claudia
Ortiz, Juan Pablo Amelio
author_facet Podio, Maricel
Rodríguez, María Pía
Felitti, Silvina
Stein, Juliana
Martínez, Erci Javier
Siena, Lorena Adelina
Quarin, Camilo Luis
Pessino, Silvina Claudia
Ortiz, Juan Pablo Amelio
author_sort Podio, Maricel
title Sequence characterization, in silico mapping and cytosine methylation analysis of markers linked to apospory in Paspalum notatum
title_short Sequence characterization, in silico mapping and cytosine methylation analysis of markers linked to apospory in Paspalum notatum
title_full Sequence characterization, in silico mapping and cytosine methylation analysis of markers linked to apospory in Paspalum notatum
title_fullStr Sequence characterization, in silico mapping and cytosine methylation analysis of markers linked to apospory in Paspalum notatum
title_full_unstemmed Sequence characterization, in silico mapping and cytosine methylation analysis of markers linked to apospory in Paspalum notatum
title_sort sequence characterization, in silico mapping and cytosine methylation analysis of markers linked to apospory in paspalum notatum
publisher Sociedade Brasileira de Genética
publishDate 2018
url http://hdl.handle.net/2133/13785
http://hdl.handle.net/2133/13785
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