Transcriptional and translational mechanisms contribute to regulate the expression of Disc Large 1 protein during different biological processes
Human discs large (DLG1) has been demonstrated to be involved in cell polarity and maintenance of tissue architecture. However, the mechanisms controlling DLG1 expression are not fully understood. This is relevant as DLG1 is lost during the later stages of malignant progression. We initiated a s...
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Autores principales: | , , , , |
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Lenguaje: | Inglés |
Publicado: |
De Gruyter
2018
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Materias: | |
Acceso en línea: | http://hdl.handle.net/2133/11431 http://hdl.handle.net/2133/11431 |
Aporte de: |
id |
I15-R121-2133-11431 |
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record_format |
dspace |
institution |
Universidad Nacional de Rosario |
institution_str |
I-15 |
repository_str |
R-121 |
collection |
Repositorio Hipermedial de la Universidad Nacional de Rosario (UNR) |
language |
Inglés |
orig_language_str_mv |
eng |
topic |
5´-UTR DLG1 Polarity Transcription Regulation Expression |
spellingShingle |
5´-UTR DLG1 Polarity Transcription Regulation Expression Marziali, Federico Emanuel Cavatorta, Ana Laura Bugnon Valdano, Marina Paula Facciuto, Florencia Natalia Gardiol, Daniela Transcriptional and translational mechanisms contribute to regulate the expression of Disc Large 1 protein during different biological processes |
topic_facet |
5´-UTR DLG1 Polarity Transcription Regulation Expression |
description |
Human discs large (DLG1) has been demonstrated
to be involved in cell polarity and maintenance of tissue
architecture. However, the mechanisms controlling DLG1
expression are not fully understood. This is relevant as
DLG1 is lost during the later stages of malignant progression. We initiated a series of studies to analyse the mechanisms regulating DLG1 expression. We have previously
reported the identification of an alternative splicing event
in the 5′ untranslated region (5′ -UTR) of DLG1 mRNA that
generates transcripts with two different 5′ -UTR (short and
large 5′ -UTR variants). In this study, we further examined
the impact of the DLG1 transcription and the role of the
differential expression of the alternative 5′ -UTRs on DLG1
protein levels. We analysed these mechanisms during cell
processes like differentiation, cell cycle progression and
cell-cell contact formation, where the importance of DLG1
activities was previously established. The data presented
in this report suggest that the transcriptional regulation
of DLG1 strongly contributes to DLG1 abundance and that
differential expression of alternative 5′ -UTRs with different translational properties, also cooperates, depending
on the cell type and cell situation. This study provides
new evidence for understanding the transcriptional regulation of DLG1 and the changes in DLG1 expression during
different biological processes |
author |
Marziali, Federico Emanuel Cavatorta, Ana Laura Bugnon Valdano, Marina Paula Facciuto, Florencia Natalia Gardiol, Daniela |
author_facet |
Marziali, Federico Emanuel Cavatorta, Ana Laura Bugnon Valdano, Marina Paula Facciuto, Florencia Natalia Gardiol, Daniela |
author_sort |
Marziali, Federico Emanuel |
title |
Transcriptional and translational mechanisms contribute to regulate the expression of Disc Large 1 protein during different biological processes |
title_short |
Transcriptional and translational mechanisms contribute to regulate the expression of Disc Large 1 protein during different biological processes |
title_full |
Transcriptional and translational mechanisms contribute to regulate the expression of Disc Large 1 protein during different biological processes |
title_fullStr |
Transcriptional and translational mechanisms contribute to regulate the expression of Disc Large 1 protein during different biological processes |
title_full_unstemmed |
Transcriptional and translational mechanisms contribute to regulate the expression of Disc Large 1 protein during different biological processes |
title_sort |
transcriptional and translational mechanisms contribute to regulate the expression of disc large 1 protein during different biological processes |
publisher |
De Gruyter |
publishDate |
2018 |
url |
http://hdl.handle.net/2133/11431 http://hdl.handle.net/2133/11431 |
work_keys_str_mv |
AT marzialifedericoemanuel transcriptionalandtranslationalmechanismscontributetoregulatetheexpressionofdisclarge1proteinduringdifferentbiologicalprocesses AT cavatortaanalaura transcriptionalandtranslationalmechanismscontributetoregulatetheexpressionofdisclarge1proteinduringdifferentbiologicalprocesses AT bugnonvaldanomarinapaula transcriptionalandtranslationalmechanismscontributetoregulatetheexpressionofdisclarge1proteinduringdifferentbiologicalprocesses AT facciutoflorencianatalia transcriptionalandtranslationalmechanismscontributetoregulatetheexpressionofdisclarge1proteinduringdifferentbiologicalprocesses AT gardioldaniela transcriptionalandtranslationalmechanismscontributetoregulatetheexpressionofdisclarge1proteinduringdifferentbiologicalprocesses |
bdutipo_str |
Repositorios |
_version_ |
1764820410002571264 |