Transcriptional and translational mechanisms contribute to regulate the expression of Disc Large 1 protein during different biological processes

Human discs large (DLG1) has been demonstrated to be involved in cell polarity and maintenance of tissue architecture. However, the mechanisms controlling DLG1 expression are not fully understood. This is relevant as DLG1 is lost during the later stages of malignant progression. We initiated a s...

Descripción completa

Guardado en:
Detalles Bibliográficos
Autores principales: Marziali, Federico Emanuel, Cavatorta, Ana Laura, Bugnon Valdano, Marina Paula, Facciuto, Florencia Natalia, Gardiol, Daniela
Lenguaje:Inglés
Publicado: De Gruyter 2018
Materias:
Acceso en línea:http://hdl.handle.net/2133/11431
http://hdl.handle.net/2133/11431
Aporte de:
id I15-R121-2133-11431
record_format dspace
institution Universidad Nacional de Rosario
institution_str I-15
repository_str R-121
collection Repositorio Hipermedial de la Universidad Nacional de Rosario (UNR)
language Inglés
orig_language_str_mv eng
topic 5´-UTR
DLG1
Polarity
Transcription Regulation
Expression
spellingShingle 5´-UTR
DLG1
Polarity
Transcription Regulation
Expression
Marziali, Federico Emanuel
Cavatorta, Ana Laura
Bugnon Valdano, Marina Paula
Facciuto, Florencia Natalia
Gardiol, Daniela
Transcriptional and translational mechanisms contribute to regulate the expression of Disc Large 1 protein during different biological processes
topic_facet 5´-UTR
DLG1
Polarity
Transcription Regulation
Expression
description Human discs large (DLG1) has been demonstrated to be involved in cell polarity and maintenance of tissue architecture. However, the mechanisms controlling DLG1 expression are not fully understood. This is relevant as DLG1 is lost during the later stages of malignant progression. We initiated a series of studies to analyse the mechanisms regulating DLG1 expression. We have previously reported the identification of an alternative splicing event in the 5′ untranslated region (5′ -UTR) of DLG1 mRNA that generates transcripts with two different 5′ -UTR (short and large 5′ -UTR variants). In this study, we further examined the impact of the DLG1 transcription and the role of the differential expression of the alternative 5′ -UTRs on DLG1 protein levels. We analysed these mechanisms during cell processes like differentiation, cell cycle progression and cell-cell contact formation, where the importance of DLG1 activities was previously established. The data presented in this report suggest that the transcriptional regulation of DLG1 strongly contributes to DLG1 abundance and that differential expression of alternative 5′ -UTRs with different translational properties, also cooperates, depending on the cell type and cell situation. This study provides new evidence for understanding the transcriptional regulation of DLG1 and the changes in DLG1 expression during different biological processes
author Marziali, Federico Emanuel
Cavatorta, Ana Laura
Bugnon Valdano, Marina Paula
Facciuto, Florencia Natalia
Gardiol, Daniela
author_facet Marziali, Federico Emanuel
Cavatorta, Ana Laura
Bugnon Valdano, Marina Paula
Facciuto, Florencia Natalia
Gardiol, Daniela
author_sort Marziali, Federico Emanuel
title Transcriptional and translational mechanisms contribute to regulate the expression of Disc Large 1 protein during different biological processes
title_short Transcriptional and translational mechanisms contribute to regulate the expression of Disc Large 1 protein during different biological processes
title_full Transcriptional and translational mechanisms contribute to regulate the expression of Disc Large 1 protein during different biological processes
title_fullStr Transcriptional and translational mechanisms contribute to regulate the expression of Disc Large 1 protein during different biological processes
title_full_unstemmed Transcriptional and translational mechanisms contribute to regulate the expression of Disc Large 1 protein during different biological processes
title_sort transcriptional and translational mechanisms contribute to regulate the expression of disc large 1 protein during different biological processes
publisher De Gruyter
publishDate 2018
url http://hdl.handle.net/2133/11431
http://hdl.handle.net/2133/11431
work_keys_str_mv AT marzialifedericoemanuel transcriptionalandtranslationalmechanismscontributetoregulatetheexpressionofdisclarge1proteinduringdifferentbiologicalprocesses
AT cavatortaanalaura transcriptionalandtranslationalmechanismscontributetoregulatetheexpressionofdisclarge1proteinduringdifferentbiologicalprocesses
AT bugnonvaldanomarinapaula transcriptionalandtranslationalmechanismscontributetoregulatetheexpressionofdisclarge1proteinduringdifferentbiologicalprocesses
AT facciutoflorencianatalia transcriptionalandtranslationalmechanismscontributetoregulatetheexpressionofdisclarge1proteinduringdifferentbiologicalprocesses
AT gardioldaniela transcriptionalandtranslationalmechanismscontributetoregulatetheexpressionofdisclarge1proteinduringdifferentbiologicalprocesses
bdutipo_str Repositorios
_version_ 1764820410002571264