Disc Large 1 expression is altered by Human Papillomavirus E7/E6 proteins in organotypic cultures of human keratinocytes

Loss of cell polarity is a fundamental process in cell transformation. Among polarity proteins, we focused on human Disc Large (DLG1), which is localized mainly at adherens junctions and contributes to the control of cell proliferation. We previously demonstrated that its expression is altered in HP...

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Detalles Bibliográficos
Autores principales:	Bugnon Valdano, Marina Paula, Cavatorta, Ana Laura, Morale, Miriam, Marziali, Federico Emanuel, Steenbergen, Renske, Boccardo, Enrique, Gardiol, Daniela
Lenguaje:	Inglés
Publicado:	Microbiology Society 2018
Materias:	HPV DLG1 Organotypic Culture
Acceso en línea:	http://hdl.handle.net/2133/10755 http://hdl.handle.net/2133/10755
Aporte de:	Repositorio Hipermedial de la Universidad Nacional de Rosario (UNR) de Universidad Nacional de Rosario

id	I15-R121-2133-10755
record_format	dspace
institution	Universidad Nacional de Rosario
institution_str	I-15
repository_str	R-121
collection	Repositorio Hipermedial de la Universidad Nacional de Rosario (UNR)
language	Inglés
orig_language_str_mv	eng
topic	HPV DLG1 Organotypic Culture
spellingShingle	HPV DLG1 Organotypic Culture Bugnon Valdano, Marina Paula Cavatorta, Ana Laura Morale, Miriam Marziali, Federico Emanuel Steenbergen, Renske Boccardo, Enrique Gardiol, Daniela Disc Large 1 expression is altered by Human Papillomavirus E7/E6 proteins in organotypic cultures of human keratinocytes
topic_facet	HPV DLG1 Organotypic Culture
description	Loss of cell polarity is a fundamental process in cell transformation. Among polarity proteins, we focused on human Disc Large (DLG1), which is localized mainly at adherens junctions and contributes to the control of cell proliferation. We previously demonstrated that its expression is altered in HPV-associated cervical neoplastic lesions, but the mechanisms beyond this remain unknown. In this study, we analyzed the contribution of HPV proteins to the changes in DLG1 expression in the squamous epithelium. We observed tissue and intracellular misdistribution of DLG1 when high-risk HPV-18 E7 or E6/E7 proteins were expressed in organotypic raft cultures. The viral oncoproteins induce the loss of DLG1 from the cell borders and an increase in the level of DLG1 protein, reflecting the pattern observed in cervical lesions. These findings were corroborated in cultures bearing the entire HPV-18 genome. Interestingly, changes in tissue distribution and abundance of DLG1 were also detected in organotypic cultures expressing the low-risk HPV-11 E7 or E6/E7 proteins; suggesting a conserved function among different HPV types. However, for low-risk HPVs, the subcellular localization of DLG1 at cell-to-cell contacts was predominantly maintained. This report offers new evidence of the involvement of HPV proteins in DLG1 expression pattern and our data support previous observations regarding DLG1 expression in cervical lesions.
author	Bugnon Valdano, Marina Paula Cavatorta, Ana Laura Morale, Miriam Marziali, Federico Emanuel Steenbergen, Renske Boccardo, Enrique Gardiol, Daniela
author_facet	Bugnon Valdano, Marina Paula Cavatorta, Ana Laura Morale, Miriam Marziali, Federico Emanuel Steenbergen, Renske Boccardo, Enrique Gardiol, Daniela
author_sort	Bugnon Valdano, Marina Paula
title	Disc Large 1 expression is altered by Human Papillomavirus E7/E6 proteins in organotypic cultures of human keratinocytes
title_short	Disc Large 1 expression is altered by Human Papillomavirus E7/E6 proteins in organotypic cultures of human keratinocytes
title_full	Disc Large 1 expression is altered by Human Papillomavirus E7/E6 proteins in organotypic cultures of human keratinocytes
title_fullStr	Disc Large 1 expression is altered by Human Papillomavirus E7/E6 proteins in organotypic cultures of human keratinocytes
title_full_unstemmed	Disc Large 1 expression is altered by Human Papillomavirus E7/E6 proteins in organotypic cultures of human keratinocytes
title_sort	disc large 1 expression is altered by human papillomavirus e7/e6 proteins in organotypic cultures of human keratinocytes
publisher	Microbiology Society
publishDate	2018
url	http://hdl.handle.net/2133/10755 http://hdl.handle.net/2133/10755
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bdutipo_str	Repositorios
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Disc Large 1 expression is altered by Human Papillomavirus E7/E6 proteins in organotypic cultures of human keratinocytes

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