Biological and physicochemical properties of bovine sodium caseinate hydrolysates obtained by a bacterial protease preparation

In this work, we aimed at the production of bovine sodium caseinate (NaCAS) hydrolysates by means of an extracellular protease from Bacillus sp. P7. Mass spectrometry was carried out to evaluate peptide mass distribution and identified sequences of peptides with a signal/noise ratio higher than 10....

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Detalles Bibliográficos
Autores principales:	Hidalgo, María Eugenia, Folmer Côrrea, Ana Paula, Mancilla Canales, Manuel Arturo, Daroit, Daniel, Brandelli, Adriano, Risso, Patricia Hilda
Formato:	article artículo publishedVersion
Lenguaje:	Inglés
Publicado:	Elsevier 2018
Materias:	Bacillus sp. P7 Bovine Sodium Caseinate Hydrolysates Bioactivity Acid Aggregation and Gelation Microstructure
Acceso en línea:	http://hdl.handle.net/2133/10477 http://hdl.handle.net/2133/10477
Aporte de:	Repositorio Hipermedial de la Universidad Nacional de Rosario (UNR) de Universidad Nacional de Rosario

id	I15-R121-2133-10477
record_format	dspace
institution	Universidad Nacional de Rosario
institution_str	I-15
repository_str	R-121
collection	Repositorio Hipermedial de la Universidad Nacional de Rosario (UNR)
language	Inglés
orig_language_str_mv	eng
topic	Bacillus sp. P7 Bovine Sodium Caseinate Hydrolysates Bioactivity Acid Aggregation and Gelation Microstructure
spellingShingle	Bacillus sp. P7 Bovine Sodium Caseinate Hydrolysates Bioactivity Acid Aggregation and Gelation Microstructure Hidalgo, María Eugenia Folmer Côrrea, Ana Paula Mancilla Canales, Manuel Arturo Daroit, Daniel Brandelli, Adriano Risso, Patricia Hilda Biological and physicochemical properties of bovine sodium caseinate hydrolysates obtained by a bacterial protease preparation
topic_facet	Bacillus sp. P7 Bovine Sodium Caseinate Hydrolysates Bioactivity Acid Aggregation and Gelation Microstructure
description	In this work, we aimed at the production of bovine sodium caseinate (NaCAS) hydrolysates by means of an extracellular protease from Bacillus sp. P7. Mass spectrometry was carried out to evaluate peptide mass distribution and identified sequences of peptides with a signal/noise ratio higher than 10. Antioxidant and antimicrobial properties of hydrolysates were evaluated. An acid-induced aggregation process of the hydrolysates and their corresponding mixtures with NaCAS were also analyzed. The results showed that the enzymatic hydrolysis produced peptides, mostly lower than 3 kDa, with different bioactivities depending on the time of hydrolysis (ti). These hydrolysates lost their ability to aggregate by addition of glucono-delta-lactone, and their incorporation into NaCAS solutions alter the kinetics of the process. Also, the degree of compactness of the NaCAS aggregates, estimated by the fractal dimension of aggregates, was not significantly altered by the incorporation of hydrolysates. However, at higher protein concentrations, when the decrease in pH leads to the formation of NaCAS acid gels, the presence of hydrolysates alters the microstructure and rheological behavior of these gels.
format	article artículo publishedVersion
author	Hidalgo, María Eugenia Folmer Côrrea, Ana Paula Mancilla Canales, Manuel Arturo Daroit, Daniel Brandelli, Adriano Risso, Patricia Hilda
author_facet	Hidalgo, María Eugenia Folmer Côrrea, Ana Paula Mancilla Canales, Manuel Arturo Daroit, Daniel Brandelli, Adriano Risso, Patricia Hilda
author_sort	Hidalgo, María Eugenia
title	Biological and physicochemical properties of bovine sodium caseinate hydrolysates obtained by a bacterial protease preparation
title_short	Biological and physicochemical properties of bovine sodium caseinate hydrolysates obtained by a bacterial protease preparation
title_full	Biological and physicochemical properties of bovine sodium caseinate hydrolysates obtained by a bacterial protease preparation
title_fullStr	Biological and physicochemical properties of bovine sodium caseinate hydrolysates obtained by a bacterial protease preparation
title_full_unstemmed	Biological and physicochemical properties of bovine sodium caseinate hydrolysates obtained by a bacterial protease preparation
title_sort	biological and physicochemical properties of bovine sodium caseinate hydrolysates obtained by a bacterial protease preparation
publisher	Elsevier
publishDate	2018
url	http://hdl.handle.net/2133/10477 http://hdl.handle.net/2133/10477
work_keys_str_mv	AT hidalgomariaeugenia biologicalandphysicochemicalpropertiesofbovinesodiumcaseinatehydrolysatesobtainedbyabacterialproteasepreparation AT folmercorreaanapaula biologicalandphysicochemicalpropertiesofbovinesodiumcaseinatehydrolysatesobtainedbyabacterialproteasepreparation AT mancillacanalesmanuelarturo biologicalandphysicochemicalpropertiesofbovinesodiumcaseinatehydrolysatesobtainedbyabacterialproteasepreparation AT daroitdaniel biologicalandphysicochemicalpropertiesofbovinesodiumcaseinatehydrolysatesobtainedbyabacterialproteasepreparation AT brandelliadriano biologicalandphysicochemicalpropertiesofbovinesodiumcaseinatehydrolysatesobtainedbyabacterialproteasepreparation AT rissopatriciahilda biologicalandphysicochemicalpropertiesofbovinesodiumcaseinatehydrolysatesobtainedbyabacterialproteasepreparation
bdutipo_str	Repositorios
_version_	1764820408406638594

Biological and physicochemical properties of bovine sodium caseinate hydrolysates obtained by a bacterial protease preparation

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