Interference of HTLV-1 Tax Protein with Cell Polarity Regulators : Defining the Subcellular Localization of the Tax-DLG1 Interaction

Human T cell leukemia virus (HTLV)-1 Tax (Tax) protein is very important in viral replication and cell transformation. Tax localizes in the nucleus and cytoplasm in association with organelles. Some activities of Tax depend on interactions with PDZ (PSD-95/Discs Large/Z0-1) domain–containing prot...

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Autores principales: Marziali, Federico Emanuel, Bugnon Valdano, Marina Paula, Brunet Avalos, Clarise, Moriena, Lucía, Cavatorta, Ana Laura, Gardiol, Daniela
Lenguaje:Inglés
Publicado: MDPI 2018
Materias:
HTLV-1
Tax
DLG1
Protein Interactions
PDZ
Acceso en línea:http://hdl.handle.net/2133/10468
http://hdl.handle.net/2133/10468
Aporte de:
Repositorio Hipermedial de la Universidad Nacional de Rosario (UNR) de Universidad Nacional de Rosario
id I15-R121-2133-10468
record_format dspace
institution Universidad Nacional de Rosario
institution_str I-15
repository_str R-121
collection Repositorio Hipermedial de la Universidad Nacional de Rosario (UNR)
language Inglés
orig_language_str_mv eng
topic HTLV-1
Tax
DLG1
Protein Interactions
PDZ
spellingShingle HTLV-1
Tax
DLG1
Protein Interactions
PDZ
Marziali, Federico Emanuel
Bugnon Valdano, Marina Paula
Brunet Avalos, Clarise
Moriena, Lucía
Cavatorta, Ana Laura
Gardiol, Daniela
Interference of HTLV-1 Tax Protein with Cell Polarity Regulators : Defining the Subcellular Localization of the Tax-DLG1 Interaction
topic_facet HTLV-1
Tax
DLG1
Protein Interactions
PDZ
description Human T cell leukemia virus (HTLV)-1 Tax (Tax) protein is very important in viral replication and cell transformation. Tax localizes in the nucleus and cytoplasm in association with organelles. Some activities of Tax depend on interactions with PDZ (PSD-95/Discs Large/Z0-1) domain–containing proteins such as Discs large protein 1 (DLG1) which is involved in cell polarity and proliferation. The DLG1 interaction results in a cytoplasmic co-localization pattern resembling vesicular aggregates, the nature of which is still unknown. To further explore the role of PDZ proteins in HTLV-1 cell transformation, we deeply investigated the Tax-DLG1 association. By fluorescence resonance energy transfer (FRET), we detected, for the first time, the direct binding of Tax to DLG1 within the cell. We showed that the interaction specifically affects the cellular distribution of not only DLG1, but also Tax. After studying different cell structures, we demonstrated that the aggregates distribute into the Golgi apparatus in spatial association with the microtubule-organizing center (MTOC). This study contributes to understand the biological significance of Tax-PDZ interactions
author Marziali, Federico Emanuel
Bugnon Valdano, Marina Paula
Brunet Avalos, Clarise
Moriena, Lucía
Cavatorta, Ana Laura
Gardiol, Daniela
author_facet Marziali, Federico Emanuel
Bugnon Valdano, Marina Paula
Brunet Avalos, Clarise
Moriena, Lucía
Cavatorta, Ana Laura
Gardiol, Daniela
author_sort Marziali, Federico Emanuel
title Interference of HTLV-1 Tax Protein with Cell Polarity Regulators : Defining the Subcellular Localization of the Tax-DLG1 Interaction
title_short Interference of HTLV-1 Tax Protein with Cell Polarity Regulators : Defining the Subcellular Localization of the Tax-DLG1 Interaction
title_full Interference of HTLV-1 Tax Protein with Cell Polarity Regulators : Defining the Subcellular Localization of the Tax-DLG1 Interaction
title_fullStr Interference of HTLV-1 Tax Protein with Cell Polarity Regulators : Defining the Subcellular Localization of the Tax-DLG1 Interaction
title_full_unstemmed Interference of HTLV-1 Tax Protein with Cell Polarity Regulators : Defining the Subcellular Localization of the Tax-DLG1 Interaction
title_sort interference of htlv-1 tax protein with cell polarity regulators : defining the subcellular localization of the tax-dlg1 interaction
publisher MDPI
publishDate 2018
url http://hdl.handle.net/2133/10468
http://hdl.handle.net/2133/10468
work_keys_str_mv AT marzialifedericoemanuel interferenceofhtlv1taxproteinwithcellpolarityregulatorsdefiningthesubcellularlocalizationofthetaxdlg1interaction
AT bugnonvaldanomarinapaula interferenceofhtlv1taxproteinwithcellpolarityregulatorsdefiningthesubcellularlocalizationofthetaxdlg1interaction
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AT gardioldaniela interferenceofhtlv1taxproteinwithcellpolarityregulatorsdefiningthesubcellularlocalizationofthetaxdlg1interaction
bdutipo_str Repositorios
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