Effect of colchicine and amiprophos-methyl on the production of in vitro doubled haploid onion plants and correlation assessment between ploidy level and stomatal size
Doubled haploid onion (Allium cepa L.) plants allow the production of completely homozygous lines for a later production of hybrids. The haploid plants are normally produced using in vitro gynogenesis. The obtained haploid plantlets must be treated with different agents for doubling chromosomes. It...
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| Autores principales: | , , , , |
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| Formato: | article Artículo publishedVersion |
| Lenguaje: | Inglés |
| Publicado: |
Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias
2013
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| Materias: | |
| Acceso en línea: | http://bdigital.uncu.edu.ar/6107 |
| Aporte de: |
| id |
I11-R86107 |
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| record_format |
dspace |
| institution |
Universidad Nacional de Cuyo |
| institution_str |
I-11 |
| repository_str |
R-8 |
| collection |
Biblioteca Digital |
| language |
Inglés |
| orig_language_str_mv |
eng |
| topic |
Colchicina Cebolla Ploidía Allium cepa Duplicación de genes Ginogénesis Híbridos Colchicine Onions Ploidy Gynogenesis Hybrids Plantas dihaploides de cebolla |
| spellingShingle |
Colchicina Cebolla Ploidía Allium cepa Duplicación de genes Ginogénesis Híbridos Colchicine Onions Ploidy Gynogenesis Hybrids Plantas dihaploides de cebolla Foschi, María Laura Martínez, Liliana Ponce, María T. Galmarini, Claudio Bohanec, Borut Effect of colchicine and amiprophos-methyl on the production of in vitro doubled haploid onion plants and correlation assessment between ploidy level and stomatal size |
| topic_facet |
Colchicina Cebolla Ploidía Allium cepa Duplicación de genes Ginogénesis Híbridos Colchicine Onions Ploidy Gynogenesis Hybrids Plantas dihaploides de cebolla |
| description |
Doubled haploid onion (Allium cepa L.)
plants allow the production of completely
homozygous lines for a later production of hybrids.
The haploid plants are normally produced using in
vitro gynogenesis. The obtained haploid plantlets
must be treated with different agents for doubling
chromosomes. It is necessary to adjust the
concentration and the length of treatment of the
doubling agent. In this case, the effect of 250 and
500 mg.L-1 colchicine and 15.2; 30 and 60 mg.L- 1
amiprophos-methyl during 24 and 48 h was
assessed over the rate of onion haploid plantlets
chromosome doubling. The best duplication
treatment was 250 mg.L-1 colchicine for 48 h,
which yielded 100% of doubled haploid plants. On
the other hand, a positive correlation resulted from
the ploidy level and stomatal size, and a negative
correlation between the level of ploidy and
stomatal density. Significant differences between
the stomatal length, width and density in haploid
and doubled haploid plantlets were observed.
An economical and quick method to test ploidy
level in onion plantlets is proposed through the
measurement of stomatal size and density. |
| format |
article Artículo Artículo publishedVersion |
| author |
Foschi, María Laura Martínez, Liliana Ponce, María T. Galmarini, Claudio Bohanec, Borut |
| author_facet |
Foschi, María Laura Martínez, Liliana Ponce, María T. Galmarini, Claudio Bohanec, Borut |
| author_sort |
Foschi, María Laura |
| title |
Effect of colchicine and amiprophos-methyl on the production of in vitro doubled haploid onion plants and correlation assessment between ploidy level and stomatal size
|
| title_short |
Effect of colchicine and amiprophos-methyl on the production of in vitro doubled haploid onion plants and correlation assessment between ploidy level and stomatal size
|
| title_full |
Effect of colchicine and amiprophos-methyl on the production of in vitro doubled haploid onion plants and correlation assessment between ploidy level and stomatal size
|
| title_fullStr |
Effect of colchicine and amiprophos-methyl on the production of in vitro doubled haploid onion plants and correlation assessment between ploidy level and stomatal size
|
| title_full_unstemmed |
Effect of colchicine and amiprophos-methyl on the production of in vitro doubled haploid onion plants and correlation assessment between ploidy level and stomatal size
|
| title_sort |
effect of colchicine and amiprophos-methyl on the production of in vitro doubled haploid onion plants and correlation assessment between ploidy level and stomatal size |
| publisher |
Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias
|
| publishDate |
2013 |
| url |
http://bdigital.uncu.edu.ar/6107 |
| work_keys_str_mv |
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Repositorios |
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