Effect of culture medium on morphogenic processes in vitro in Cinchona officinalis L.
This paper describes the effect of the various plant growth regulators of the culture medium on morphogenic processes in vitro in Cinchona officinalis L, a highly vulnerable species from southern Ecuador. To do this, different concentrations of NaOCl were used in combination with different immersion...
Guardado en:
| Autores principales: | , , , |
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| Formato: | article Artículo publishedVersion |
| Lenguaje: | Inglés |
| Publicado: |
Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias
2019
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| Materias: | |
| Acceso en línea: | http://bdigital.uncu.edu.ar/13400 |
| Aporte de: |
| id |
I11-R813400 |
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| record_format |
dspace |
| institution |
Universidad Nacional de Cuyo |
| institution_str |
I-11 |
| repository_str |
R-8 |
| collection |
Biblioteca Digital |
| language |
Inglés |
| orig_language_str_mv |
eng |
| topic |
Germinación Cultivo in vitro Cinchona officinalis Callogénesis Organogénesis Sustancias de crecimiento vegetal Plant growth Germination Organogenesis Callogenesis Reguladores del crecimiento |
| spellingShingle |
Germinación Cultivo in vitro Cinchona officinalis Callogénesis Organogénesis Sustancias de crecimiento vegetal Plant growth Germination Organogenesis Callogenesis Reguladores del crecimiento Moreno Serrano, José Antonio Pérez Ruíz, César Moreno Fierro, Ivonne Moreno Fierro, Jorge Effect of culture medium on morphogenic processes in vitro in Cinchona officinalis L. |
| topic_facet |
Germinación Cultivo in vitro Cinchona officinalis Callogénesis Organogénesis Sustancias de crecimiento vegetal Plant growth Germination Organogenesis Callogenesis Reguladores del crecimiento |
| description |
This paper describes the effect of the various plant growth regulators of the culture
medium on morphogenic processes in vitro in Cinchona officinalis L, a highly vulnerable
species from southern Ecuador. To do this, different concentrations of NaOCl were used in
combination with different immersion times for seed disinfection; for seed germination
in vitro GA3 was added to the MS basal culture medium in different concentrations, and
for morphogenic processes in vitro, different concentrations of auxins and cytokinins
were combined. The decrease in the contamination rate was with high concentrations
of NaOCl and an increase in the germination rate in 45 days with the addition of 1.0 mg
L-1 GA3 to the culture medium the hormonal combination of 0.5 mg L-1 NAA + 2.5 mg L-1
BAP showed a high rate of shoot proliferation and with 1.0 mg L-1 NAA a high number
of roots was obtained. In the callogenesis phase, the best results were obtained with 1.0
mg L-1 2,4-D + 0.5 mg L-1 BAP for callus proliferation. In vitro propagation protocols were
generated in Cinchona officinalis L, for the preservation and conservation of the species. |
| format |
article Artículo Artículo publishedVersion |
| author |
Moreno Serrano, José Antonio Pérez Ruíz, César Moreno Fierro, Ivonne Moreno Fierro, Jorge |
| author_facet |
Moreno Serrano, José Antonio Pérez Ruíz, César Moreno Fierro, Ivonne Moreno Fierro, Jorge |
| author_sort |
Moreno Serrano, José Antonio |
| title |
Effect of culture medium on morphogenic processes in vitro in Cinchona officinalis L.
|
| title_short |
Effect of culture medium on morphogenic processes in vitro in Cinchona officinalis L.
|
| title_full |
Effect of culture medium on morphogenic processes in vitro in Cinchona officinalis L.
|
| title_fullStr |
Effect of culture medium on morphogenic processes in vitro in Cinchona officinalis L.
|
| title_full_unstemmed |
Effect of culture medium on morphogenic processes in vitro in Cinchona officinalis L.
|
| title_sort |
effect of culture medium on morphogenic processes in vitro in cinchona officinalis l. |
| publisher |
Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias
|
| publishDate |
2019 |
| url |
http://bdigital.uncu.edu.ar/13400 |
| work_keys_str_mv |
AT morenoserranojoseantonio effectofculturemediumonmorphogenicprocessesinvitroincinchonaofficinalisl AT perezruizcesar effectofculturemediumonmorphogenicprocessesinvitroincinchonaofficinalisl AT morenofierroivonne effectofculturemediumonmorphogenicprocessesinvitroincinchonaofficinalisl AT morenofierrojorge effectofculturemediumonmorphogenicprocessesinvitroincinchonaofficinalisl AT morenoserranojoseantonio efectodelmediodecultivoenlosprocesosmorfogenicosinvitroencinchonaofficinalisl AT perezruizcesar efectodelmediodecultivoenlosprocesosmorfogenicosinvitroencinchonaofficinalisl AT morenofierroivonne efectodelmediodecultivoenlosprocesosmorfogenicosinvitroencinchonaofficinalisl AT morenofierrojorge efectodelmediodecultivoenlosprocesosmorfogenicosinvitroencinchonaofficinalisl |
| bdutipo_str |
Repositorios |
| _version_ |
1764820403737329667 |