Influence of cytoplasm on in vitro regeneration capacity in hybrids and their segregating generations in tomato (Lycopersicon esculentum Mill)

Three tomato cultivars (Lycopersicon esculentum Mill): Rossol Selección La Consulta (R); UC 82 (U) and Platauco INTA (P), their reciprocal crosses were studied for their shoot-forming capacity. Leaf explants were cultured in vitro on Murashige and Skoog (MS) medium with AIA 0.175 mg/L + BA 2.25 mg/L...

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Detalles Bibliográficos
Autores principales: Zorzoli, R., Cointry, E. L., Prado, E. A., Mroginski, L. A., Picardi, L. A.
Formato: Artículo revista
Lenguaje:Español
Publicado: Facultad de Ciencias Agropecuarias 1992
Materias:
tomato
hybrids
shoot-forming capacity
cytoplasm
tomates
híbridos
capacidad de regeneración
citoplasma
Acceso en línea:https://revistas.unc.edu.ar/index.php/agris/article/view/2941
Aporte de:
AgriScientia de Universidad Nacional de Córdoba
id I10-R352-article-2941
record_format ojs
spelling I10-R352-article-29412024-09-06T15:05:33Z Influence of cytoplasm on in vitro regeneration capacity in hybrids and their segregating generations in tomato (Lycopersicon esculentum Mill) Influencia del citoplasma sobre la capacidad de regeneración in vitro en híbridos y sus generaciones segregantes en tomate (Lycopersicon esculentum Mill) Zorzoli, R. Cointry, E. L. Prado, E. A. Mroginski, L. A. Picardi, L. A. tomato hybrids shoot-forming capacity cytoplasm tomates híbridos capacidad de regeneración citoplasma Three tomato cultivars (Lycopersicon esculentum Mill): Rossol Selección La Consulta (R); UC 82 (U) and Platauco INTA (P), their reciprocal crosses were studied for their shoot-forming capacity. Leaf explants were cultured in vitro on Murashige and Skoog (MS) medium with AIA 0.175 mg/L + BA 2.25 mg/L (M1) and with BA 1.125 mg/L (MII). Shoot-forming capacity was evaluated through two measures: the development rate (DR), proportion of explants with calli and/or shoots, and prolificity rate (PR), proportion of shoots to developed explants. No differences among cultivars were found in both media for DR and PR. In M1, significant differences between reciprocal crosses (R x U - U x R) were found for PR (t = 1.8; p < 0.05). F2 plants were obtained by selfing the F1 and 171 of the former had the U cytoplasm, while 85 had the R one. The U cytoplasm PR was 2.43 and R was 1.93 (t = 2.81; p < 0.01). These results would indicate that the cytoplasm effects on regeneration are still active in the F2 generation, since it can be inferred that the genetic variability for the regeneration ability might be inhibited by cytoplasmic effects. Con el objeto de estudiar la influencia del citoplasma sobre la capacidad de regeneración in vitro en tomate, se usaron tres cultivares de tomate (Rossol Selección La Consulta (R); UC 82 (U) y Platauco INTA (P), sus cruzas recíprocas y las F2. Los explantos foliares se cultivaron sobre el medio Murashige y Skoog (MS) con 0,175 mg/L de AIA y 2,25 mg/L de BA (MI) y 1,125 mg/L de BA (MII). La capacidad de regeneración fue evaluada a través de 2 variables: la tasa de desarrollo (TD), proporción de explantos con callos y/o vástagos, y la tasa de prolificidad (TP), proporción de vástagos por explantos desarrollados. No se encontraron diferencias en los cultivares entre ambos medios para TD ni para TP. En el MI se manifestaron diferencias para TP entre las cruzas recíprocas (R x U y U x R) (t = 1,8; p < 0,05). Las plantas F2 se obtuvieron por autofecundación de las F1. En la generación segregante, las plantas con el citoplasma U presentaron una TP de 2,43 y para el citoplasma R de 1,93 (t = 2,81; p < 0,01).  Facultad de Ciencias Agropecuarias 1992-07-01 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf https://revistas.unc.edu.ar/index.php/agris/article/view/2941 10.31047/1668.298x.v9.n2.2941 AgriScientia; Vol. 9 No. 2 (1992); 15-19 AgriScientia; Vol. 9 Núm. 2 (1992); 15-19 1668-298X 10.31047/1668.298x.v9.n2 spa https://revistas.unc.edu.ar/index.php/agris/article/view/2941/2767 Derechos de autor 1992 R. Zorzoli, E.L. Cointry, E.A. Prado, L.A. Mroginski, L.A. Picardi. https://creativecommons.org/licenses/by-sa/4.0
institution Universidad Nacional de Córdoba
institution_str I-10
repository_str R-352
container_title_str AgriScientia
language Español
format Artículo revista
topic tomato
hybrids
shoot-forming capacity
cytoplasm
tomates
híbridos
capacidad de regeneración
citoplasma
spellingShingle tomato
hybrids
shoot-forming capacity
cytoplasm
tomates
híbridos
capacidad de regeneración
citoplasma
Zorzoli, R.
Cointry, E. L.
Prado, E. A.
Mroginski, L. A.
Picardi, L. A.
Influence of cytoplasm on in vitro regeneration capacity in hybrids and their segregating generations in tomato (Lycopersicon esculentum Mill)
topic_facet tomato
hybrids
shoot-forming capacity
cytoplasm
tomates
híbridos
capacidad de regeneración
citoplasma
author Zorzoli, R.
Cointry, E. L.
Prado, E. A.
Mroginski, L. A.
Picardi, L. A.
author_facet Zorzoli, R.
Cointry, E. L.
Prado, E. A.
Mroginski, L. A.
Picardi, L. A.
author_sort Zorzoli, R.
title Influence of cytoplasm on in vitro regeneration capacity in hybrids and their segregating generations in tomato (Lycopersicon esculentum Mill)
title_short Influence of cytoplasm on in vitro regeneration capacity in hybrids and their segregating generations in tomato (Lycopersicon esculentum Mill)
title_full Influence of cytoplasm on in vitro regeneration capacity in hybrids and their segregating generations in tomato (Lycopersicon esculentum Mill)
title_fullStr Influence of cytoplasm on in vitro regeneration capacity in hybrids and their segregating generations in tomato (Lycopersicon esculentum Mill)
title_full_unstemmed Influence of cytoplasm on in vitro regeneration capacity in hybrids and their segregating generations in tomato (Lycopersicon esculentum Mill)
title_sort influence of cytoplasm on in vitro regeneration capacity in hybrids and their segregating generations in tomato (lycopersicon esculentum mill)
description Three tomato cultivars (Lycopersicon esculentum Mill): Rossol Selección La Consulta (R); UC 82 (U) and Platauco INTA (P), their reciprocal crosses were studied for their shoot-forming capacity. Leaf explants were cultured in vitro on Murashige and Skoog (MS) medium with AIA 0.175 mg/L + BA 2.25 mg/L (M1) and with BA 1.125 mg/L (MII). Shoot-forming capacity was evaluated through two measures: the development rate (DR), proportion of explants with calli and/or shoots, and prolificity rate (PR), proportion of shoots to developed explants. No differences among cultivars were found in both media for DR and PR. In M1, significant differences between reciprocal crosses (R x U - U x R) were found for PR (t = 1.8; p < 0.05). F2 plants were obtained by selfing the F1 and 171 of the former had the U cytoplasm, while 85 had the R one. The U cytoplasm PR was 2.43 and R was 1.93 (t = 2.81; p < 0.01). These results would indicate that the cytoplasm effects on regeneration are still active in the F2 generation, since it can be inferred that the genetic variability for the regeneration ability might be inhibited by cytoplasmic effects.
publisher Facultad de Ciencias Agropecuarias
publishDate 1992
url https://revistas.unc.edu.ar/index.php/agris/article/view/2941
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first_indexed 2024-09-03T22:15:56Z
last_indexed 2024-09-10T02:51:40Z
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