Histological and histomorphometric analysis of bone repair of rat jaws using alendronate and aloe vera gels

The maintenance of bone mass in the jaws is a concern for dental professionals. Objective: To study the effect of local administration of Alendronate (AL) and Aloe vera (AV) on the tissue regeneration of the post-exodontia alveolus in rats. Materials and methods: Absorbable gelatin hemostatic sponge...

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Autores principales: De Leonardi, Gabriela Marina, Aguzzi , Alejandra Cecilia, Virga, María Carolina, De Leonardi, Adriana Ruth María, Moro, Cecilia
Formato: Artículo revista
Lenguaje:Español
Publicado: Facultad de Odontología 2023
Materias:
alendronato
aloe vera
reparación ósea
alendronate
bone repair
Acceso en línea:https://revistas.unc.edu.ar/index.php/RevFacOdonto/article/view/43420
Aporte de:
Revista de la Facultad de Odontología de Universidad Nacional de Córdoba
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institution Universidad Nacional de Córdoba
institution_str I-10
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container_title_str Revista de la Facultad de Odontología
language Español
format Artículo revista
topic alendronato
aloe vera
reparación ósea
alendronate
aloe vera
bone repair
spellingShingle alendronato
aloe vera
reparación ósea
alendronate
aloe vera
bone repair
De Leonardi, Gabriela Marina
Aguzzi , Alejandra Cecilia
Virga, María Carolina
De Leonardi, Adriana Ruth María
Moro, Cecilia
Histological and histomorphometric analysis of bone repair of rat jaws using alendronate and aloe vera gels
topic_facet alendronato
aloe vera
reparación ósea
alendronate
aloe vera
bone repair
author De Leonardi, Gabriela Marina
Aguzzi , Alejandra Cecilia
Virga, María Carolina
De Leonardi, Adriana Ruth María
Moro, Cecilia
author_facet De Leonardi, Gabriela Marina
Aguzzi , Alejandra Cecilia
Virga, María Carolina
De Leonardi, Adriana Ruth María
Moro, Cecilia
author_sort De Leonardi, Gabriela Marina
title Histological and histomorphometric analysis of bone repair of rat jaws using alendronate and aloe vera gels
title_short Histological and histomorphometric analysis of bone repair of rat jaws using alendronate and aloe vera gels
title_full Histological and histomorphometric analysis of bone repair of rat jaws using alendronate and aloe vera gels
title_fullStr Histological and histomorphometric analysis of bone repair of rat jaws using alendronate and aloe vera gels
title_full_unstemmed Histological and histomorphometric analysis of bone repair of rat jaws using alendronate and aloe vera gels
title_sort histological and histomorphometric analysis of bone repair of rat jaws using alendronate and aloe vera gels
description The maintenance of bone mass in the jaws is a concern for dental professionals. Objective: To study the effect of local administration of Alendronate (AL) and Aloe vera (AV) on the tissue regeneration of the post-exodontia alveolus in rats. Materials and methods: Absorbable gelatin hemostatic sponges were used; they were embedded with a solution of LA (0.5 mg/Kg) and 70% AV gel; control group (C) used saline. The effect was evaluated in male Wistar rats (n=64), divided into 4 groups: C, AL, AV and AL+AV. The first lower molars were extracted, the embedded hemostatic sponges were placed in the alveoli. The handling of the animals was carried out following the norms established by SECyT according to CICUAL. Determinations were made at 0, 7, 15, 30 days. The lower jaw was extracted, placed in a sterile jar and fixed in 10% formalin; the samples were decalcified and then embedded in paraffin. Histological sections were made and stained with Hematoxylin/Eosin. Histological and histomorphometric parameters were analyzed. An analysis of Variance (ANOVA) was performed for two classification criteria. Results: In the histomorphological analysis, at 15 days the AV group presented greater new formation of bone tissue. At 30 days, the presence of mature bone with the presence of Haversian systems was observed in the AV and AL+AV group. The histomorphometric study at 15 and 30 days showed significant differences in the number of osteoblasts per linear mm, between the AV and AL+AV group with respect to group C (p<0.01). When measuring trabecular thickness, significant differences were observed at 30 days between the AV group and the C group (p<0.01). Conclusions: The parameters studied demonstrated that Aloe vera administered locally favored bone repair in the post-exodontia alveoli.
publisher Facultad de Odontología
publishDate 2023
url https://revistas.unc.edu.ar/index.php/RevFacOdonto/article/view/43420
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first_indexed 2024-09-03T21:16:33Z
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spelling I10-R335-article-434202025-09-26T13:24:36Z Histological and histomorphometric analysis of bone repair of rat jaws using alendronate and aloe vera gels Análisis histológico e histomorfométrico de la reparación ósea de mandíbulas de ratas usando geles de alendronato y aloe vera De Leonardi, Gabriela Marina Aguzzi , Alejandra Cecilia Virga, María Carolina De Leonardi, Adriana Ruth María Moro, Cecilia alendronato aloe vera reparación ósea alendronate aloe vera bone repair The maintenance of bone mass in the jaws is a concern for dental professionals. Objective: To study the effect of local administration of Alendronate (AL) and Aloe vera (AV) on the tissue regeneration of the post-exodontia alveolus in rats. Materials and methods: Absorbable gelatin hemostatic sponges were used; they were embedded with a solution of LA (0.5 mg/Kg) and 70% AV gel; control group (C) used saline. The effect was evaluated in male Wistar rats (n=64), divided into 4 groups: C, AL, AV and AL+AV. The first lower molars were extracted, the embedded hemostatic sponges were placed in the alveoli. The handling of the animals was carried out following the norms established by SECyT according to CICUAL. Determinations were made at 0, 7, 15, 30 days. The lower jaw was extracted, placed in a sterile jar and fixed in 10% formalin; the samples were decalcified and then embedded in paraffin. Histological sections were made and stained with Hematoxylin/Eosin. Histological and histomorphometric parameters were analyzed. An analysis of Variance (ANOVA) was performed for two classification criteria. Results: In the histomorphological analysis, at 15 days the AV group presented greater new formation of bone tissue. At 30 days, the presence of mature bone with the presence of Haversian systems was observed in the AV and AL+AV group. The histomorphometric study at 15 and 30 days showed significant differences in the number of osteoblasts per linear mm, between the AV and AL+AV group with respect to group C (p<0.01). When measuring trabecular thickness, significant differences were observed at 30 days between the AV group and the C group (p<0.01). Conclusions: The parameters studied demonstrated that Aloe vera administered locally favored bone repair in the post-exodontia alveoli. El mantenimiento de la masa ósea de los maxilares constituye una preocupación para los profesionales odontólogos. Objetivo: Estudiar el efecto de la administración local de Alendronato (AL) y Aloe vera (AV) en la regeneración tisular del alvéolo post exodoncia en ratas. Materiales y métodos: Se utilizaron esponjas hemostáticas absorbibles de gelatina; se embebieron con una solución de AL (0.5 mg/Kg) y gel de AV al 70%; el grupo control (C) usó solución salina. El efecto se evaluó en ratas machos Wistar (n=64), divididos en 4 grupos: C, AL, AV y AL+AV. Se realizó la exodoncia de primeros molares inferiores, en los alvéolos se colocaron las esponjas hemostáticas embebidas. El manejo de los animales se realizó siguiendo las normas establecidas por SECyT según CICUAL. Se efectuaron determinaciones a los 0, 7, 15, 30 días. Se extrajo el maxilar inferior, fue colocado en frasco estéril y fijado en formol al 10%; las muestras fueron descalcificadas y luego incluidas en parafina. Se hicieron cortes histológicos y se colorearon con Hematoxilina/Eosina. Se analizaron parámetros histológicos e histomorfométricos. Se realizó un análisis de Varianza (ANOVA) a dos criterios de clasificación. Resultados: En el análisis histomorfológico, a los 15 días el grupo AV presentó mayor neoformación de tejido óseo. A los 30 días se observó la presencia de hueso maduro con presencia de sistemas de Havers en el grupo AV y AL+AV. En el estudio histomorfométrico a los 15 y 30 días se evidencian diferencias significativas en el número de osteoblastos por mm lineal, entre el grupo AV y AL+AV con respecto al grupo C (p<0,01). Al medir espesor trabecular se observó a los 30 días diferencias significativas entre el grupo AV y el grupo C (p<0,01). Conclusiones: Los parámetros estudiados demostraron que Aloe vera administrado localmente favoreció la reparación ósea en los alvéolos post exodoncia. Facultad de Odontología 2023-12-05 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf https://revistas.unc.edu.ar/index.php/RevFacOdonto/article/view/43420 Revista de la Facultad de Odontología; Vol. 33 Núm. 3 (2023): Rev Fac Odont 33(3)-2023-Diciembre; 13-20 2545-7594 0325-1071 spa https://revistas.unc.edu.ar/index.php/RevFacOdonto/article/view/43420/43392 10.25014/ Derechos de autor 2023 Gabriela Marina De Leonardi, Alejandra Cecilia Aguzzi , María Carolina Virga, Adriana Ruth María De Leonardi, Cecilia Moro https://creativecommons.org/licenses/by/4.0

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