Evaluation of an antimicrobial on the adhesion of Candida albicans to denture materials
Objective: to evaluate the effect of AlphaSan® on C. albicans adhesion to acrylic and polyamide resins. Methods: 2 x 2 mm squares were made with acrylic resin (AR) and polyamide resin (PR). Four study groups (n=12) were established: Group A: AR without AlphaSan®, Group B: AR with AlphaSan®, Group C:...
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| Autores principales: | , , |
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| Formato: | Artículo revista |
| Lenguaje: | Español |
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Facultad de Odontología
2023
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| Materias: | |
| Acceso en línea: | https://revistas.unc.edu.ar/index.php/RevFacOdonto/article/view/41988 |
| Aporte de: |
| id |
I10-R335-article-41988 |
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| record_format |
ojs |
| institution |
Universidad Nacional de Córdoba |
| institution_str |
I-10 |
| repository_str |
R-335 |
| container_title_str |
Revista de la Facultad de Odontología |
| language |
Español |
| format |
Artículo revista |
| topic |
dental materials AlphaSan Candida albicans materiales dentales AlphaSan Candida albicans |
| spellingShingle |
dental materials AlphaSan Candida albicans materiales dentales AlphaSan Candida albicans Vera, Mónica Mabel Pascualini, Carlos José Bojanich, María Alejandra Evaluation of an antimicrobial on the adhesion of Candida albicans to denture materials |
| topic_facet |
dental materials AlphaSan Candida albicans materiales dentales AlphaSan Candida albicans |
| author |
Vera, Mónica Mabel Pascualini, Carlos José Bojanich, María Alejandra |
| author_facet |
Vera, Mónica Mabel Pascualini, Carlos José Bojanich, María Alejandra |
| author_sort |
Vera, Mónica Mabel |
| title |
Evaluation of an antimicrobial on the adhesion of Candida albicans to denture materials |
| title_short |
Evaluation of an antimicrobial on the adhesion of Candida albicans to denture materials |
| title_full |
Evaluation of an antimicrobial on the adhesion of Candida albicans to denture materials |
| title_fullStr |
Evaluation of an antimicrobial on the adhesion of Candida albicans to denture materials |
| title_full_unstemmed |
Evaluation of an antimicrobial on the adhesion of Candida albicans to denture materials |
| title_sort |
evaluation of an antimicrobial on the adhesion of candida albicans to denture materials |
| description |
Objective: to evaluate the effect of AlphaSan® on C. albicans adhesion to acrylic and polyamide resins. Methods: 2 x 2 mm squares were made with acrylic resin (AR) and polyamide resin (PR). Four study groups (n=12) were established: Group A: AR without AlphaSan®, Group B: AR with AlphaSan®, Group C: RP without AlphaSan®, Group D: RP with AlphaSan®. All groups were incubated in BHI broth with C. albicans ATCC at 36°C for 48 hours. Quantification of fungal adherence was performed by confocal microscopy (CM) and by means of an aqueous solution of crystal violet (CV), measuring the absorbance at 570 nm. Results: In the quantification of C. albicans adherence with CM, a 50% decrease (p =0.03) was observed between the treated groups (B and D) in relation to those not treated with AlphaSan® (A and C). No significant difference in C. albicans adherence was found between groups B and D (p > 0.5). C. albicans adherence, by CV, decreased by 55% (p= 0.01) in AR with AlphaSan® compared to AR without AlphaSan®. On the other hand, it was observed that fungal adherence decreased by 61% (p=0.01) in RP with AlphaSan® compared to RP without AlphaSan®. No significant difference was observed between the samples treated with the antifungal studied (p >0.05). Conclusion: Fungal adherence to the materials treated with AlphaSan® was limited to 50%, this would be due to the fact that the fungus presents a rigid cell wall (glucans and chitin), which provides a physical barrier for the penetration of different compounds. Consequently, it could be assumed that AlphaSan® would act on the cell wall, which would result in 50% of the fungi being able to adhere to the dental materials. |
| publisher |
Facultad de Odontología |
| publishDate |
2023 |
| url |
https://revistas.unc.edu.ar/index.php/RevFacOdonto/article/view/41988 |
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AT veramonicamabel evaluationofanantimicrobialontheadhesionofcandidaalbicanstodenturematerials AT pascualinicarlosjose evaluationofanantimicrobialontheadhesionofcandidaalbicanstodenturematerials AT bojanichmariaalejandra evaluationofanantimicrobialontheadhesionofcandidaalbicanstodenturematerials AT veramonicamabel evaluaciondeunantimicrobianosobrelaadhesiondecandidaalbicansamaterialesdeprotesisdentales AT pascualinicarlosjose evaluaciondeunantimicrobianosobrelaadhesiondecandidaalbicansamaterialesdeprotesisdentales AT bojanichmariaalejandra evaluaciondeunantimicrobianosobrelaadhesiondecandidaalbicansamaterialesdeprotesisdentales |
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2024-09-03T21:16:31Z |
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2025-10-10T05:06:50Z |
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I10-R335-article-419882025-09-29T13:54:49Z Evaluation of an antimicrobial on the adhesion of Candida albicans to denture materials Evaluación de un antimicrobiano sobre la adhesión de Candida albicans a materiales de prótesis dentales Vera, Mónica Mabel Pascualini, Carlos José Bojanich, María Alejandra dental materials AlphaSan Candida albicans materiales dentales AlphaSan Candida albicans Objective: to evaluate the effect of AlphaSan® on C. albicans adhesion to acrylic and polyamide resins. Methods: 2 x 2 mm squares were made with acrylic resin (AR) and polyamide resin (PR). Four study groups (n=12) were established: Group A: AR without AlphaSan®, Group B: AR with AlphaSan®, Group C: RP without AlphaSan®, Group D: RP with AlphaSan®. All groups were incubated in BHI broth with C. albicans ATCC at 36°C for 48 hours. Quantification of fungal adherence was performed by confocal microscopy (CM) and by means of an aqueous solution of crystal violet (CV), measuring the absorbance at 570 nm. Results: In the quantification of C. albicans adherence with CM, a 50% decrease (p =0.03) was observed between the treated groups (B and D) in relation to those not treated with AlphaSan® (A and C). No significant difference in C. albicans adherence was found between groups B and D (p > 0.5). C. albicans adherence, by CV, decreased by 55% (p= 0.01) in AR with AlphaSan® compared to AR without AlphaSan®. On the other hand, it was observed that fungal adherence decreased by 61% (p=0.01) in RP with AlphaSan® compared to RP without AlphaSan®. No significant difference was observed between the samples treated with the antifungal studied (p >0.05). Conclusion: Fungal adherence to the materials treated with AlphaSan® was limited to 50%, this would be due to the fact that the fungus presents a rigid cell wall (glucans and chitin), which provides a physical barrier for the penetration of different compounds. Consequently, it could be assumed that AlphaSan® would act on the cell wall, which would result in 50% of the fungi being able to adhere to the dental materials. Objetivo: evaluar el efecto de AlphaSan® sobre la adhesión C. albicans a resinas acrílicas y poliamidas. Métodos: se realizaron cuadrados de 2 x 2 mm con resina acrílica (RA) y con resina de poliamida (RP). Se establecieron 4 grupos estudios (n=12): Grupo A: RA sin AlphaSan®, Grupo B: RA con AlphaSan®, Grupo C: RP sin AlphaSan®, Grupo D: RP con AlphaSan®. Todos los grupos se incubaron en caldo BHI con C. albicans ATCC a 36°C durante 48 horas. La cuantificación de la adherencia fúngica se realizó por microscopía confocal (MC) y mediante una solución acuosa de cristal violeta (CV), mediendo la absorbancia a 570 nm. Resultados: En la cuantificación de la adherencia de C. albicans con MC, se observó una disminución del 50% (p =0,03) entre los grupos tratados (B y D) en relación a los no tratados con AlphaSan® (A y C). No se encontró diferencia significativa de la adherencia de C. albicans entre los grupos B y D (p > 0,5). La adherencia de C. albicans, mediante CV, disminuyó en un 55% (p= 0,01) en la RA con AlphaSan® comparada con RA sin AlphaSan®. Por otro lado, se observó que en la RP con AlphaSan® disminuyó la adherencia fúngica en un 61% (p =0,01) en relación a la RP sin AlphaSan®. No se observó diferencia significativa entre las muestras tratadas con el antifúgico estudiado (p >0,05). Conclusión: La adherencia fúngica en los materiales tratados con AlphaSan®se limitó a un 50%, esto se debería a que el hongo presenta una pared celular rígida (glucanos y quitina), que proporciona una barrera física para la penetración de diferentes compuestos. En consecuencia, podría suponerse que el AlphaSan® actuaria sobre la pared celular, lo que se traduciría que un 50% de los hongos sean capaces de adherirse a los materiales dentales. Facultad de Odontología 2023-08-03 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf https://revistas.unc.edu.ar/index.php/RevFacOdonto/article/view/41988 Revista de la Facultad de Odontología; Vol. 33 Núm. 2 (2023): Rev Fac Odont 32(2)-2023-Agosto; 3-8 2545-7594 0325-1071 spa https://revistas.unc.edu.ar/index.php/RevFacOdonto/article/view/41988/42130 10.25014/ Derechos de autor 2023 Mónica Mabel Vera, Carlos José Pascualini, María Alejandra Bojanich https://creativecommons.org/licenses/by/4.0 |