Selective fluorescence study of salivary pleomorphic adenoma stroma with confocal laser microscope in stained slices with Hematoxylin / Eosine yellow
Introduction: The pleomorphic adenoma (PA) is a benign salivary gland tumor. It has ductal cells,myoepitheliocytes and a stroma with hyalinised, myxoid and chondroid metaplasia zones. On the other hand, the H/ E gives an integral view of the tissue structure. Hematoxylin acts as a basic dye that sta...
Guardado en:
| Autor principal: | |
|---|---|
| Formato: | Artículo revista |
| Lenguaje: | Español |
| Publicado: |
Facultad de Odontología
2019
|
| Materias: | |
| Acceso en línea: | https://revistas.unc.edu.ar/index.php/RevFacOdonto/article/view/26882 |
| Aporte de: |
| id |
I10-R335-article-26882 |
|---|---|
| record_format |
ojs |
| institution |
Universidad Nacional de Córdoba |
| institution_str |
I-10 |
| repository_str |
R-335 |
| container_title_str |
Revista de la Facultad de Odontología |
| language |
Español |
| format |
Artículo revista |
| topic |
photonic microscope,confocal laser scanning microscope, H/E, fluorescence, pleomorphic adenoma, stroma microscopia fotónica, microscopia confocal, H/E, fluorescencia, adenoma pleomórfico, estroma |
| spellingShingle |
photonic microscope,confocal laser scanning microscope, H/E, fluorescence, pleomorphic adenoma, stroma microscopia fotónica, microscopia confocal, H/E, fluorescencia, adenoma pleomórfico, estroma Gomez Rosso et al, María Araceli Selective fluorescence study of salivary pleomorphic adenoma stroma with confocal laser microscope in stained slices with Hematoxylin / Eosine yellow |
| topic_facet |
photonic microscope,confocal laser scanning microscope, H/E, fluorescence, pleomorphic adenoma, stroma microscopia fotónica, microscopia confocal, H/E, fluorescencia, adenoma pleomórfico, estroma |
| author |
Gomez Rosso et al, María Araceli |
| author_facet |
Gomez Rosso et al, María Araceli |
| author_sort |
Gomez Rosso et al, María Araceli |
| title |
Selective fluorescence study of salivary pleomorphic adenoma stroma with confocal laser microscope in stained slices with Hematoxylin / Eosine yellow |
| title_short |
Selective fluorescence study of salivary pleomorphic adenoma stroma with confocal laser microscope in stained slices with Hematoxylin / Eosine yellow |
| title_full |
Selective fluorescence study of salivary pleomorphic adenoma stroma with confocal laser microscope in stained slices with Hematoxylin / Eosine yellow |
| title_fullStr |
Selective fluorescence study of salivary pleomorphic adenoma stroma with confocal laser microscope in stained slices with Hematoxylin / Eosine yellow |
| title_full_unstemmed |
Selective fluorescence study of salivary pleomorphic adenoma stroma with confocal laser microscope in stained slices with Hematoxylin / Eosine yellow |
| title_sort |
selective fluorescence study of salivary pleomorphic adenoma stroma with confocal laser microscope in stained slices with hematoxylin / eosine yellow |
| description |
Introduction: The pleomorphic adenoma (PA) is a benign salivary gland tumor. It has ductal cells,myoepitheliocytes and a stroma with hyalinised, myxoid and chondroid metaplasia zones. On the other hand, the H/ E gives an integral view of the tissue structure. Hematoxylin acts as a basic dye that stains violet. The eosin is an artificial acid dye that stains pink eosinophilic structures. The Eosina Y is strongly fluorescent, which allows to identify with the confocal laser microscope eosinophilic structures that are sometimes little visible to the photonic microscope. Objective: The PA chondroid stroma was analyzed with photonic and confocal scanning laser microscopes to demonstrate the value of the fluorescence emitted by Eosin Y in its structural description. Material & Methods: The histological examination of 10 PA parotid stained with H/E Y was performed. The images of the histological preparations were obtained by means of a photonic optical microscope and a FV1000 Olympus spectral confocal laser microscope, configured for the acquisition of orange / red fluorescence of the Eosin obtained between 555 to 655 nm. The eosin was excited at 543 nm using the He-Ne gas laser. Results: Photonic microscopy: The chondroid tumor areas presented a variable extension predominantly in only 4 cases. Histologically, these areas looked like immature hyaline cartilage, a chondroid type with an amorphous-like basophilic extracellular matrix and lacunae with round or oval chondroidocytes. * Scanning confocal laser microscopy: structures with strong red fluorescence were observed swollen in the extracellular matrix, the pericellular capsule of the chondroidocytes and the collagen of the territorial and inter-territorial matrices. Conclusions: The confocal laser scanning microscope allows visualizing structures in PA slides stained with H/E Y not visible to the clear field. We highlight the usefulness of H/E Y in histopathology both in photonic microscopy and confocal laser scanning microscopy. |
| publisher |
Facultad de Odontología |
| publishDate |
2019 |
| url |
https://revistas.unc.edu.ar/index.php/RevFacOdonto/article/view/26882 |
| work_keys_str_mv |
AT gomezrossoetalmariaaraceli selectivefluorescencestudyofsalivarypleomorphicadenomastromawithconfocallasermicroscopeinstainedsliceswithhematoxylineosineyellow AT gomezrossoetalmariaaraceli estudiodelafluorescenciaselectivadelestromadeladenomapleomorficosalivalconmicroscopiolaserconfocalenpreparacionescoloreadasconhematoxilinaeosinay |
| first_indexed |
2024-09-03T21:15:44Z |
| last_indexed |
2024-09-03T21:15:44Z |
| _version_ |
1809211084949684224 |
| spelling |
I10-R335-article-268822019-12-20T08:52:50Z Selective fluorescence study of salivary pleomorphic adenoma stroma with confocal laser microscope in stained slices with Hematoxylin / Eosine yellow Estudio de la fluorescencia selectiva del estroma del adenoma pleomórfico salival con microscopio laser confocal en preparaciones coloreadas con Hematoxilina/ Eosina Y Gomez Rosso et al, María Araceli photonic microscope,confocal laser scanning microscope, H/E, fluorescence, pleomorphic adenoma, stroma microscopia fotónica, microscopia confocal, H/E, fluorescencia, adenoma pleomórfico, estroma Introduction: The pleomorphic adenoma (PA) is a benign salivary gland tumor. It has ductal cells,myoepitheliocytes and a stroma with hyalinised, myxoid and chondroid metaplasia zones. On the other hand, the H/ E gives an integral view of the tissue structure. Hematoxylin acts as a basic dye that stains violet. The eosin is an artificial acid dye that stains pink eosinophilic structures. The Eosina Y is strongly fluorescent, which allows to identify with the confocal laser microscope eosinophilic structures that are sometimes little visible to the photonic microscope. Objective: The PA chondroid stroma was analyzed with photonic and confocal scanning laser microscopes to demonstrate the value of the fluorescence emitted by Eosin Y in its structural description. Material & Methods: The histological examination of 10 PA parotid stained with H/E Y was performed. The images of the histological preparations were obtained by means of a photonic optical microscope and a FV1000 Olympus spectral confocal laser microscope, configured for the acquisition of orange / red fluorescence of the Eosin obtained between 555 to 655 nm. The eosin was excited at 543 nm using the He-Ne gas laser. Results: Photonic microscopy: The chondroid tumor areas presented a variable extension predominantly in only 4 cases. Histologically, these areas looked like immature hyaline cartilage, a chondroid type with an amorphous-like basophilic extracellular matrix and lacunae with round or oval chondroidocytes. * Scanning confocal laser microscopy: structures with strong red fluorescence were observed swollen in the extracellular matrix, the pericellular capsule of the chondroidocytes and the collagen of the territorial and inter-territorial matrices. Conclusions: The confocal laser scanning microscope allows visualizing structures in PA slides stained with H/E Y not visible to the clear field. We highlight the usefulness of H/E Y in histopathology both in photonic microscopy and confocal laser scanning microscopy. Introducción: El adenoma pleomórfico (AP) es un tumor benigno de glándulas salivales. Presenta células ductales y mioepiteliocitos y un estroma con zonas hialinizadas, mixoides y metaplasia condroide. Por otro lado, la H/E da una visión integral de la estructura tisular. La Hematoxilina actúa como colorante básica que tiñe de color violeta. La eosina es un colorante ácido artificial que tiñe de rosado estructuras eosinofílicas. La EosinaY es fuertemente fluorescente, lo que permite identificar con el microscopio laser confocal estructuras eosinofílicas que a veces son poco visibles al microscopio fotónico. Objetivo: Se analizó con microscopía fotónica y laser confocal de barrido el estroma condroide del AP para demostrar el valor de la fluorescencia emitida por la EosinaY en su descripción estructural. Material y Métodos: Se realizó el examen histológico de 10 AP de parótida coloreado con H/EY. Las imágenes de las preparaciones histológicas fueron obtenidas por medio de un microscopio óptico fotónico y un microscopio laser confocal espectral FV1000 Olympus, configurado para la adquisición de fluorescencia naranja/roja de la Eosina obtenida entre 555 a 655 nm. La eosina fue excitada a 543 nm empleando el láser gaseosode He-Ne. Resultados: Microscopía fotónica: Las áreas tumorales condroides presentaron una extensión variable predominando en sólo 4 casos. Histológicamente estas áreas tenían el aspecto de cartílago hialino inmaduro, tipo condroide, con una matriz extracelular basófila de apariencia amorfa y lagunas con condroidocitos redondos u ovales. Microscopía laser confocal de barrido: se observaron estructuras con fuerte fluorescencia roja sobresaliendo en la matriz extracelular, la cápsula pericelular de los condrocitos y el componente colágeno de las matrices territorial e interterritorial. Conclusiones: El microscopio confocal permite visualizar estructuras en preparaciones de AP coloreadas con H/EY no visibles con campo claro. Destacamos la utilidad de la H/EY en histopatología tanto en microscopía fotónica como confocal de barrido. Facultad de Odontología 2019-12-20 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf https://revistas.unc.edu.ar/index.php/RevFacOdonto/article/view/26882 Revista de la Facultad de Odontología; Vol. 29 Núm. 3 (2019): Volumen 29 número 3; 13-17 2545-7594 0325-1071 spa https://revistas.unc.edu.ar/index.php/RevFacOdonto/article/view/26882/28567 |