Structural variations produced IN VITRO by gonadotrophins and steroid hormones on the cell surfaces of the ovarian epithelium of the chick embryo

The purpose of the present work was to analyze the structural variations producen in vitro" by hormones on thccli surfaces of the ovarian c'pitheliuni of the chick embryo, in relation to the growth of the left ovary and atrophy of the right one. Explants of both ovaries lirom embryos at 7...

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Autores principales: >, Ávila , Rodolfo, Samar, María, P de Fabro, Sofía
Formato: Artículo revista
Lenguaje:Español
Publicado: Universidad Nacional Córdoba. Facultad de Ciencias Médicas. Secretaria de Ciencia y Tecnología 1991
Materias:
ovaries
chick embryo
gonadotrophins
Steroids
cultures
Acceso en línea:https://revistas.unc.edu.ar/index.php/med/article/view/45078
Aporte de:
Revista de la Facultad de Ciencias Médicas de Córdoba de Universidad Nacional de Córdoba
id I10-R327-article-45078
record_format ojs
spelling I10-R327-article-450782024-11-27T20:39:36Z Structural variations produced IN VITRO by gonadotrophins and steroid hormones on the cell surfaces of the ovarian epithelium of the chick embryo > Ávila , Rodolfo Samar, María P de Fabro, Sofía ovaries chick embryo gonadotrophins Steroids cultures The purpose of the present work was to analyze the structural variations producen in vitro" by hormones on thccli surfaces of the ovarian c'pitheliuni of the chick embryo, in relation to the growth of the left ovary and atrophy of the right one. Explants of both ovaries lirom embryos at 7 to 19 days of development were separately cultured for 4 days in MEM with 10 % of fetal bovinc serum (control), and plus 17- /3-estradiol, testosterone propionate, progestcrone, FSH, LH or hCG added indiviclually to cultures (experimental). The cultures were processecl for thei st.ructural, ultrastructural and cytocheniical study. In control cultures, membrane differentiations and mucosubstan(eS \vere similar in both ovarics and at all ages to those in ovo. 17-/9-estradiol produced a greater development of microvilli, junci ional complexes and mucinc in the epitbelial eclis and interdigitations in the germ celis of the left ovary, while in the right gonad there was ccli regression. Testosterorie ami progesterone evoked in the left gonad a response similar to that obtainecl with Ihe eStrOgen, whereas in the right ovary no changes were observed with respe't t) controis. FSH lcd to cellular regression in both ovaries. Finally. with L or hCG the changes produced in thleft ovary were similar to those induccd by the estrogen, and in the right one an mercase of membrane differentiations and of mucosubstances ielatcd lo Iliem vas found in comparison to conirois. The rcsults obtainecl in expiants of ovarics at 7 days of development and cultured for 4 days, inclicatc that the modifications of the ccli suri aces of the, ovarian epitheliurn of the chick embryo would he related to the action of steroid and gonadotrophic hormones at the start of gonaclal differentiation. Universidad Nacional Córdoba. Facultad de Ciencias Médicas. Secretaria de Ciencia y Tecnología 1991-11-20 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf https://revistas.unc.edu.ar/index.php/med/article/view/45078 10.31053/1853.0605.v49.n2.45078 Revista de la Facultad de Ciencias Médicas de Córdoba.; Vol. 49 No. 2 (1991); 7-12 Revista de la Facultad de Ciencias Médicas de Córdoba; Vol. 49 Núm. 2 (1991); 7-12 Revista da Faculdade de Ciências Médicas de Córdoba; v. 49 n. 2 (1991); 7-12 1853-0605 0014-6722 10.31053/1853.0605.v49.n2 spa https://revistas.unc.edu.ar/index.php/med/article/view/45078/45096 Derechos de autor 2024 Universidad Nacional de Córdoba http://creativecommons.org/licenses/by-nc/4.0
institution Universidad Nacional de Córdoba
institution_str I-10
repository_str R-327
container_title_str Revista de la Facultad de Ciencias Médicas de Córdoba
language Español
format Artículo revista
topic ovaries
chick embryo
gonadotrophins
Steroids
cultures
spellingShingle ovaries
chick embryo
gonadotrophins
Steroids
cultures
>
Ávila , Rodolfo
Samar, María
P de Fabro, Sofía
Structural variations produced IN VITRO by gonadotrophins and steroid hormones on the cell surfaces of the ovarian epithelium of the chick embryo
topic_facet ovaries
chick embryo
gonadotrophins
Steroids
cultures
author >
Ávila , Rodolfo
Samar, María
P de Fabro, Sofía
author_facet >
Ávila , Rodolfo
Samar, María
P de Fabro, Sofía
author_sort >
title Structural variations produced IN VITRO by gonadotrophins and steroid hormones on the cell surfaces of the ovarian epithelium of the chick embryo
title_short Structural variations produced IN VITRO by gonadotrophins and steroid hormones on the cell surfaces of the ovarian epithelium of the chick embryo
title_full Structural variations produced IN VITRO by gonadotrophins and steroid hormones on the cell surfaces of the ovarian epithelium of the chick embryo
title_fullStr Structural variations produced IN VITRO by gonadotrophins and steroid hormones on the cell surfaces of the ovarian epithelium of the chick embryo
title_full_unstemmed Structural variations produced IN VITRO by gonadotrophins and steroid hormones on the cell surfaces of the ovarian epithelium of the chick embryo
title_sort structural variations produced in vitro by gonadotrophins and steroid hormones on the cell surfaces of the ovarian epithelium of the chick embryo
description The purpose of the present work was to analyze the structural variations producen in vitro" by hormones on thccli surfaces of the ovarian c'pitheliuni of the chick embryo, in relation to the growth of the left ovary and atrophy of the right one. Explants of both ovaries lirom embryos at 7 to 19 days of development were separately cultured for 4 days in MEM with 10 % of fetal bovinc serum (control), and plus 17- /3-estradiol, testosterone propionate, progestcrone, FSH, LH or hCG added indiviclually to cultures (experimental). The cultures were processecl for thei st.ructural, ultrastructural and cytocheniical study. In control cultures, membrane differentiations and mucosubstan(eS \vere similar in both ovarics and at all ages to those in ovo. 17-/9-estradiol produced a greater development of microvilli, junci ional complexes and mucinc in the epitbelial eclis and interdigitations in the germ celis of the left ovary, while in the right gonad there was ccli regression. Testosterorie ami progesterone evoked in the left gonad a response similar to that obtainecl with Ihe eStrOgen, whereas in the right ovary no changes were observed with respe't t) controis. FSH lcd to cellular regression in both ovaries. Finally. with L or hCG the changes produced in thleft ovary were similar to those induccd by the estrogen, and in the right one an mercase of membrane differentiations and of mucosubstances ielatcd lo Iliem vas found in comparison to conirois. The rcsults obtainecl in expiants of ovarics at 7 days of development and cultured for 4 days, inclicatc that the modifications of the ccli suri aces of the, ovarian epitheliurn of the chick embryo would he related to the action of steroid and gonadotrophic hormones at the start of gonaclal differentiation.
publisher Universidad Nacional Córdoba. Facultad de Ciencias Médicas. Secretaria de Ciencia y Tecnología
publishDate 1991
url https://revistas.unc.edu.ar/index.php/med/article/view/45078
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AT samarmaria structuralvariationsproducedinvitrobygonadotrophinsandsteroidhormonesonthecellsurfacesoftheovarianepitheliumofthechickembryo
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