Breast cancer: effects of protumoral dietary patterns on oncogenic and oncosuppressive microRNA expression
MicroRNAs or miRs, Oncogenic (OncomiR) and Oncosuppressive, participate in the regulation of gene expression and present a particular deregulation in different types of cancer. Given that dietary habits would have a role in the occurrence of cancer, the interest arises to study the effect of pr...
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| Autores principales: | , , , , , , |
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| Formato: | Artículo revista |
| Lenguaje: | Español |
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Universidad Nacional Córdoba. Facultad de Ciencias Médicas. Secretaria de Ciencia y Tecnología
2023
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| Acceso en línea: | https://revistas.unc.edu.ar/index.php/med/article/view/42791 |
| Aporte de: |
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I10-R327-article-42791 |
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ojs |
| institution |
Universidad Nacional de Córdoba |
| institution_str |
I-10 |
| repository_str |
R-327 |
| container_title_str |
Revista de la Facultad de Ciencias Médicas de Córdoba |
| language |
Español |
| format |
Artículo revista |
| topic |
breast cancer miRNAs and gene expression regulation dietary patterns cáncer de mama miARNs y regulación génica patrones dietarios |
| spellingShingle |
breast cancer miRNAs and gene expression regulation dietary patterns cáncer de mama miARNs y regulación génica patrones dietarios Pérez de Rosas, AR García, BA Gareis, T Sosa, LV Pasquialini, ME Quintar, A Stroppa, MM Breast cancer: effects of protumoral dietary patterns on oncogenic and oncosuppressive microRNA expression |
| topic_facet |
breast cancer miRNAs and gene expression regulation dietary patterns cáncer de mama miARNs y regulación génica patrones dietarios |
| author |
Pérez de Rosas, AR García, BA Gareis, T Sosa, LV Pasquialini, ME Quintar, A Stroppa, MM |
| author_facet |
Pérez de Rosas, AR García, BA Gareis, T Sosa, LV Pasquialini, ME Quintar, A Stroppa, MM |
| author_sort |
Pérez de Rosas, AR |
| title |
Breast cancer: effects of protumoral dietary patterns on oncogenic and oncosuppressive microRNA expression |
| title_short |
Breast cancer: effects of protumoral dietary patterns on oncogenic and oncosuppressive microRNA expression |
| title_full |
Breast cancer: effects of protumoral dietary patterns on oncogenic and oncosuppressive microRNA expression |
| title_fullStr |
Breast cancer: effects of protumoral dietary patterns on oncogenic and oncosuppressive microRNA expression |
| title_full_unstemmed |
Breast cancer: effects of protumoral dietary patterns on oncogenic and oncosuppressive microRNA expression |
| title_sort |
breast cancer: effects of protumoral dietary patterns on oncogenic and oncosuppressive microrna expression |
| description |
MicroRNAs or miRs, Oncogenic (OncomiR) and Oncosuppressive, participate in the regulation of gene expression and present a particular deregulation in different types of cancer. Given that dietary habits would have a role in the occurrence of cancer, the interest arises to study the effect of protumoral dietary patterns on the expression of miRs commonly deregulated in a characteristic way in breast cancer.
We investigated the influence of the dietary components fructose (F), palmitic acid (PA) and mixture (F+PA) on miRs expression in cultured MCF7 breast cancer cells and Carcinoma Associated Fibroblasts F88 (CAF-F88) and miRs expression was analyzed in a murine model of breast cancer in the context of high fructose (PBA), high fat (PCS) and a mixture of both (PBA+PCS) diets. Extraction of miRs from culture and serum samples was performed and expression levels were determined by retrotranscription and quantitative polymerase chain reaction (RTqPCR) using the Taqman primer and probe system specific for the OncomiRs miR 21 and miR 155 and the miR Oncosuppressor miRs miR let7a and miR 195.
In MCF7 cells, miRs expression was mainly affected by F+AP treatment. In CAF-F88 cells, overexpression of OncomiRs was observed with AP treatment and a decrease in OncoSuppressor miRs with F+AP treatment. In serum from the murine experimental model, a significant increase of the OncomiR miR21 and a decrease of the Oncosuppressor miR-Let7a were observed in the group treated with the PBA+PCS diet.
The observed modifications in the expression of miRs commonly deregulated in breast cancer demonstrate the involvement of miRs as one of the underlying regulatory molecular mechanisms that would favor the development of tumor processes and their proliferation in nutritional contexts and protumor diets.
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| publisher |
Universidad Nacional Córdoba. Facultad de Ciencias Médicas. Secretaria de Ciencia y Tecnología |
| publishDate |
2023 |
| url |
https://revistas.unc.edu.ar/index.php/med/article/view/42791 |
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2024-09-03T21:05:01Z |
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2024-09-03T21:05:01Z |
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I10-R327-article-427912023-10-19T21:19:08Z Breast cancer: effects of protumoral dietary patterns on oncogenic and oncosuppressive microRNA expression Cáncer de mama: efectos de patrones dietarios protumorales en la expresión de microARNs Oncogénicos y Oncosupresores Pérez de Rosas, AR García, BA Gareis, T Sosa, LV Pasquialini, ME Quintar, A Stroppa, MM breast cancer miRNAs and gene expression regulation dietary patterns cáncer de mama miARNs y regulación génica patrones dietarios MicroRNAs or miRs, Oncogenic (OncomiR) and Oncosuppressive, participate in the regulation of gene expression and present a particular deregulation in different types of cancer. Given that dietary habits would have a role in the occurrence of cancer, the interest arises to study the effect of protumoral dietary patterns on the expression of miRs commonly deregulated in a characteristic way in breast cancer. We investigated the influence of the dietary components fructose (F), palmitic acid (PA) and mixture (F+PA) on miRs expression in cultured MCF7 breast cancer cells and Carcinoma Associated Fibroblasts F88 (CAF-F88) and miRs expression was analyzed in a murine model of breast cancer in the context of high fructose (PBA), high fat (PCS) and a mixture of both (PBA+PCS) diets. Extraction of miRs from culture and serum samples was performed and expression levels were determined by retrotranscription and quantitative polymerase chain reaction (RTqPCR) using the Taqman primer and probe system specific for the OncomiRs miR 21 and miR 155 and the miR Oncosuppressor miRs miR let7a and miR 195. In MCF7 cells, miRs expression was mainly affected by F+AP treatment. In CAF-F88 cells, overexpression of OncomiRs was observed with AP treatment and a decrease in OncoSuppressor miRs with F+AP treatment. In serum from the murine experimental model, a significant increase of the OncomiR miR21 and a decrease of the Oncosuppressor miR-Let7a were observed in the group treated with the PBA+PCS diet. The observed modifications in the expression of miRs commonly deregulated in breast cancer demonstrate the involvement of miRs as one of the underlying regulatory molecular mechanisms that would favor the development of tumor processes and their proliferation in nutritional contexts and protumor diets. Los microARNs o miRs, Oncogénicos (OncomiR) y Oncosupresores, participan en la regulación de expresión génica y presentan una desregulación particular en los distintos tipos de cáncer. Dado que los hábitos dietarios tendrían un rol en la ocurrencia de cáncer, surge el interés de estudiar el efecto de patrones dietarios protumorales sobre la expresión de miRs comúnmente desregulados de forma característica en el cáncer de mama. Se investigó la influencia de los componentes dietarios fructosa (F), ácido palmítico (AP) y mezcla (F+AP) en la expresión de miRs en cultivos de células de cáncer de mama MCF7 y de Fibroblastos Asociados a Carcinoma F88 (CAF-F88) y se analizó la expresión de miRs en un modelo murino de cáncer de mama en el contexto de dietas rica en fructosa (PBA), rica en grasas (PCS) y con una mezcla de ambas (PBA+PCS). Se realizó extracción de miRs a partir de las muestras obtenidas de cultivo y suero y los niveles de expresión se determinaron mediante la técnica de retrotranscripción y reacción en cadena de la polimerasa cuantitativa (RTqPCR), utilizando el sistema de primers y sondas Taqman específicas para los OncomiRs miR 21 y miR 155 y los miR Oncosupresores miR let7a y miR 195. Se observó sobrexpresión de OncomiRs y disminución en la expresión de miR Oncosupresores. En células MCF7 la expresión de miRs fue afectada principalmente por el tratamiento con F+AP. En células CAF-F88 se observó sobrexpresión de OncomiRs con el tratamiento con AP y disminución de miR OncoSupresores con el tratamiento con F+AP. En suero del modelo experimental murino, se observó un incremento significativo del OncomiR miR21 y una disminución del miR Oncosupresor miR-Let7a en el grupo tratado con la dieta PBA+PCS. Las modificaciones observadas en la expresión de miRs comúnmente desregulados en el cáncer de mama demuestran la participación de los miRs como uno de los mecanismos moleculares regulatorios subyacentes que favorecerían el desarrollo de procesos tumorales y su proliferación en contextos nutricionales y dietas protumorales. Universidad Nacional Córdoba. Facultad de Ciencias Médicas. Secretaria de Ciencia y Tecnología 2023-10-19 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf https://revistas.unc.edu.ar/index.php/med/article/view/42791 Revista de la Facultad de Ciencias Médicas de Córdoba.; Vol. 80 (2023): Suplemento JIC XXIV Revista de la Facultad de Ciencias Médicas de Córdoba; Vol. 80 (2023): Suplemento JIC XXIV Revista da Faculdade de Ciências Médicas de Córdoba; v. 80 (2023): Suplemento JIC XXIV 1853-0605 0014-6722 spa https://revistas.unc.edu.ar/index.php/med/article/view/42791/42826 Derechos de autor 2023 Universidad Nacional de Córdoba http://creativecommons.org/licenses/by-nc/4.0 |