Effects of DEHP phthalate on the expression of proteins that are part of the blood-testis barrier.
Toxicological studies have revealed that exposure to an anthropogenic environmental contaminant, di-2-ethylhexyl phthalate (DEHP), during development induces both morphological and functional alterations of the reproductive system through mechanisms that are not y...
| Autores principales: | , , , , |
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| Formato: | Artículo revista |
| Lenguaje: | Español |
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Universidad Nacional Córdoba. Facultad de Ciencias Médicas. Secretaria de Ciencia y Tecnología
2023
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| Materias: | |
| Acceso en línea: | https://revistas.unc.edu.ar/index.php/med/article/view/42664 |
| Aporte de: |
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I10-R327-article-42664 |
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ojs |
| institution |
Universidad Nacional de Córdoba |
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I-10 |
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R-327 |
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Revista de la Facultad de Ciencias Médicas de Córdoba |
| language |
Español |
| format |
Artículo revista |
| topic |
Endocrine disruption phthalate testicle FILAMIN A claudin-1 disrupción endocrina FTALATO testículo FILAMINA A claudina-1 |
| spellingShingle |
Endocrine disruption phthalate testicle FILAMIN A claudin-1 disrupción endocrina FTALATO testículo FILAMINA A claudina-1 Vitellini, F Pérez, P Toledo, J Silva, T Gutiérrez, S Effects of DEHP phthalate on the expression of proteins that are part of the blood-testis barrier. |
| topic_facet |
Endocrine disruption phthalate testicle FILAMIN A claudin-1 disrupción endocrina FTALATO testículo FILAMINA A claudina-1 |
| author |
Vitellini, F Pérez, P Toledo, J Silva, T Gutiérrez, S |
| author_facet |
Vitellini, F Pérez, P Toledo, J Silva, T Gutiérrez, S |
| author_sort |
Vitellini, F |
| title |
Effects of DEHP phthalate on the expression of proteins that are part of the blood-testis barrier. |
| title_short |
Effects of DEHP phthalate on the expression of proteins that are part of the blood-testis barrier. |
| title_full |
Effects of DEHP phthalate on the expression of proteins that are part of the blood-testis barrier. |
| title_fullStr |
Effects of DEHP phthalate on the expression of proteins that are part of the blood-testis barrier. |
| title_full_unstemmed |
Effects of DEHP phthalate on the expression of proteins that are part of the blood-testis barrier. |
| title_sort |
effects of dehp phthalate on the expression of proteins that are part of the blood-testis barrier. |
| description |
Toxicological studies have revealed that exposure to an anthropogenic environmental contaminant, di-2-ethylhexyl phthalate (DEHP), during development induces both morphological and functional alterations of the reproductive system through mechanisms that are not yet fully understood. This has prompted an analysis of the effects of DEHP on testicular development, involving an evaluation of Filamin A (FLNA) expression, a protein integral to the assembly of the blood-testis barrier (BTB), and claudin-1, a protein pivotal for the structural integrity of this barrier.
Male Wistar rats were exposed to either 200 μg/ml of DEHP (the standard exposure dose) or corn oil (used as controls) (n=15/group) throughout gestation and lactation, following a maternal exposure model. The animals were sacrificed at 21 and 75 postnatal days (during the prepubertal and adulthood periods, respectively), and the testicles were processed for ultrastructural analysis of germ cells using transmission electron microscopy, detection of FLNA through immunohistochemistry, quantification of FLNA-expressing cell numbers using flow cytometry, and analysis of claudin-1 via western blot. Data analysis involved the application of Analysis of Variance (ANOVA), followed by Tukey's test to identify differences in means.
The results revealed that exposure to DEHP during development and lactation led to a reduction in the number of testicular cells expressing FLNA, accompanied by a decrease in the expression of FLNA and claudin-1 proteins within prepubertal animal testicles. In adulthood, DEHP inhibited the expression of FLNA and claudin-1 proteins, consequently inducing disorganization of the maturation layers of the germinal epithelium, along with degenerative alterations at the ultrastructural level of the germinal progeny.
These results indicate that exposure to DEHP during gestation and lactation affects testicular development, disrupting the assembly and structure of the blood-testis barrier (BTB), providing new insights into the impact of a pervasive endocrine disruptor and the triggering mechanism on the testis. |
| publisher |
Universidad Nacional Córdoba. Facultad de Ciencias Médicas. Secretaria de Ciencia y Tecnología |
| publishDate |
2023 |
| url |
https://revistas.unc.edu.ar/index.php/med/article/view/42664 |
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I10-R327-article-426642023-10-19T21:20:09Z Effects of DEHP phthalate on the expression of proteins that are part of the blood-testis barrier. Efectos del ftalato DEHP sobre la expresión de proteínas que forman parte de la barrera hemato-testicular Vitellini, F Pérez, P Toledo, J Silva, T Gutiérrez, S Endocrine disruption phthalate testicle FILAMIN A claudin-1 disrupción endocrina FTALATO testículo FILAMINA A claudina-1 Toxicological studies have revealed that exposure to an anthropogenic environmental contaminant, di-2-ethylhexyl phthalate (DEHP), during development induces both morphological and functional alterations of the reproductive system through mechanisms that are not yet fully understood. This has prompted an analysis of the effects of DEHP on testicular development, involving an evaluation of Filamin A (FLNA) expression, a protein integral to the assembly of the blood-testis barrier (BTB), and claudin-1, a protein pivotal for the structural integrity of this barrier. Male Wistar rats were exposed to either 200 μg/ml of DEHP (the standard exposure dose) or corn oil (used as controls) (n=15/group) throughout gestation and lactation, following a maternal exposure model. The animals were sacrificed at 21 and 75 postnatal days (during the prepubertal and adulthood periods, respectively), and the testicles were processed for ultrastructural analysis of germ cells using transmission electron microscopy, detection of FLNA through immunohistochemistry, quantification of FLNA-expressing cell numbers using flow cytometry, and analysis of claudin-1 via western blot. Data analysis involved the application of Analysis of Variance (ANOVA), followed by Tukey's test to identify differences in means. The results revealed that exposure to DEHP during development and lactation led to a reduction in the number of testicular cells expressing FLNA, accompanied by a decrease in the expression of FLNA and claudin-1 proteins within prepubertal animal testicles. In adulthood, DEHP inhibited the expression of FLNA and claudin-1 proteins, consequently inducing disorganization of the maturation layers of the germinal epithelium, along with degenerative alterations at the ultrastructural level of the germinal progeny. These results indicate that exposure to DEHP during gestation and lactation affects testicular development, disrupting the assembly and structure of the blood-testis barrier (BTB), providing new insights into the impact of a pervasive endocrine disruptor and the triggering mechanism on the testis. Estudios toxicológicos han mostrado que la exposición a un contaminante ambiental de origen antrópico, el ftalato di-2-etilhexil ftalato (DEHP) durante el desarrollo induce alteraciones morfológicas y funcionales del aparato reproductor por mecanismos no del todo conocidos. Esto motivó a analizar los efectos del DEHP durante el desarrollo sobre el testículo, evaluando la expresión de Filamina A (FLNA), una proteína que participa en el ensamblado de la barrera hemato-testicular (BHT) y de claudina-1, una proteína con una importante función estructural de esta barrera. Se utilizaron ratas macho Wistar expuestas a 200 ug/ml de DEHP (dosis de exposición habitual) o con aceite de maíz (vehículo, como controles) (n=15/grupo) durante toda la gestación y la lactancia (modelo de exposición materna). Los animales fueron sacrificados a los 21 y 75 días posnatal (DPN, período prepuberal y adultez respectivamente) y los testículos fueron procesados para el análisis ultraestructural de las células germinales por microscopía electrónica de transmisión, la detección de FLNA por inmunohistoquímica, la cuantificación del número de células que expresan FLNA por citometría de flujo y el análisis de claudina-1 por western blot. El análisis de los datos se llevó a cabo aplicando Análisis de Varianza (ANOVA) y posteriormente las diferencias entre medias se analizaron mediante el Test de Tukey. Los resultados mostraron que la exposición al DEHP durante el desarrollo y la lactancia redujo el número de células testiculares que expresaron FLNA así como disminuyó la expresión de las proteínas FLNA y claudina-1 en testículos de animales prepuberales. En la adultez el DEHP inhibió la expresión de las proteínas FLNA y claudina-1 e indujo desorganización de las capas madurativas del epitelio germinal, con cambios degenerativos a nivel ultraestructural de la progenie germinal. Estos resultados muestran que la exposición al DEHP durante la gestación y lactancia afecta el desarrollo testicular, alterando el ensamblado y la estructura de la BHT, proporcionando nueva información sobre los efectos de un disruptor endocrino ubicuo y el mecanismo desencadenado sobre el testículo. Universidad Nacional Córdoba. Facultad de Ciencias Médicas. Secretaria de Ciencia y Tecnología 2023-10-19 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf https://revistas.unc.edu.ar/index.php/med/article/view/42664 Revista de la Facultad de Ciencias Médicas de Córdoba.; Vol. 80 (2023): Suplemento JIC XXIV Revista de la Facultad de Ciencias Médicas de Córdoba; Vol. 80 (2023): Suplemento JIC XXIV Revista da Faculdade de Ciências Médicas de Córdoba; v. 80 (2023): Suplemento JIC XXIV 1853-0605 0014-6722 spa https://revistas.unc.edu.ar/index.php/med/article/view/42664/42861 Derechos de autor 2023 Universidad Nacional de Córdoba http://creativecommons.org/licenses/by-nc/4.0 |