Optimized chromatographic methods for the identification and quantification of terpenes in Cannabis sativa oil for medicinal use
Introduction: Cannabis sativa is a plant species with numerous active principles, so the list of its therapeutic uses is expanding. In this sense, there are numerous evidences of the possible medicinal use of terpenes, as well as their synergism with cannabinoids (entourage effect). Thus, as more co...
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| Formato: | Artículo revista |
| Lenguaje: | Español |
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Universidad Nacional Córdoba. Facultad de Ciencias Médicas. Secretaria de Ciencia y Tecnología
2023
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| Acceso en línea: | https://revistas.unc.edu.ar/index.php/med/article/view/39593 |
| Aporte de: |
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I10-R327-article-39593 |
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Universidad Nacional de Córdoba |
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Revista de la Facultad de Ciencias Médicas de Córdoba |
| language |
Español |
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Artículo revista |
| topic |
terpenes gas chromatography quality control Cannabis sativa terpenos cromatografía gaseosa control de calidad cannabis sativa terpenos cromatografia de gás calidad controle de qualidade |
| spellingShingle |
terpenes gas chromatography quality control Cannabis sativa terpenos cromatografía gaseosa control de calidad cannabis sativa terpenos cromatografia de gás calidad controle de qualidade Bajda, Leonardo Amaro, María Marcela Bongiovanni, Guillermina A. Optimized chromatographic methods for the identification and quantification of terpenes in Cannabis sativa oil for medicinal use |
| topic_facet |
terpenes gas chromatography quality control Cannabis sativa terpenos cromatografía gaseosa control de calidad cannabis sativa terpenos cromatografia de gás calidad controle de qualidade |
| author |
Bajda, Leonardo Amaro, María Marcela Bongiovanni, Guillermina A. |
| author_facet |
Bajda, Leonardo Amaro, María Marcela Bongiovanni, Guillermina A. |
| author_sort |
Bajda, Leonardo |
| title |
Optimized chromatographic methods for the identification and quantification of terpenes in Cannabis sativa oil for medicinal use |
| title_short |
Optimized chromatographic methods for the identification and quantification of terpenes in Cannabis sativa oil for medicinal use |
| title_full |
Optimized chromatographic methods for the identification and quantification of terpenes in Cannabis sativa oil for medicinal use |
| title_fullStr |
Optimized chromatographic methods for the identification and quantification of terpenes in Cannabis sativa oil for medicinal use |
| title_full_unstemmed |
Optimized chromatographic methods for the identification and quantification of terpenes in Cannabis sativa oil for medicinal use |
| title_sort |
optimized chromatographic methods for the identification and quantification of terpenes in cannabis sativa oil for medicinal use |
| description |
Introduction: Cannabis sativa is a plant species with numerous active principles, so the list of its therapeutic uses is expanding. In this sense, there are numerous evidences of the possible medicinal use of terpenes, as well as their synergism with cannabinoids (entourage effect). Thus, as more countries contemplate the legalization and authorization of medical cannabis, the number of cannabis extraction and analysis laboratories is increasing to meet the demand, requiring adequate analytical tools. Methodology: In response to numerous inquiries from physicians, analytical laboratories and users, the PROBIEN chromatography laboratory has selected two methods for the analysis of terpenes in Cannabis oil by gas chromatography technique (GC-FID). The methods are described using HP-5 and Innowax columns. The external standard method was used for the quantitative determination of β-Pinene, Myrcene, p-Cymene, Limonene, Linalool, α-Terpineol, Nerol and Geraniol. Results: good peak separation and reproducibility were observed, appropriate for the identification and quantification of the main terpenes in Cannabis extracts. The area/concentration ratio was linear in the range of 0.0005 to 2.0 mg/ml. Main conclusion: The described methods allow the identification and quantification of the major terpenes in Cannabis oil for an adequate quality control. |
| publisher |
Universidad Nacional Córdoba. Facultad de Ciencias Médicas. Secretaria de Ciencia y Tecnología |
| publishDate |
2023 |
| url |
https://revistas.unc.edu.ar/index.php/med/article/view/39593 |
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I10-R327-article-395932023-07-10T12:07:38Z Optimized chromatographic methods for the identification and quantification of terpenes in Cannabis sativa oil for medicinal use Métodos cromatográficos optimizados para la identificación y cuantificación de terpenos en aceite de Cannabis sativa de uso medicinal Métodos cromatográficos otimizado para a identificação e quantificação de terpenos em óleo de Cannabis sativa para uso médico Bajda, Leonardo Amaro, María Marcela Bongiovanni, Guillermina A. terpenes gas chromatography quality control Cannabis sativa terpenos cromatografía gaseosa control de calidad cannabis sativa terpenos cromatografia de gás calidad controle de qualidade Introduction: Cannabis sativa is a plant species with numerous active principles, so the list of its therapeutic uses is expanding. In this sense, there are numerous evidences of the possible medicinal use of terpenes, as well as their synergism with cannabinoids (entourage effect). Thus, as more countries contemplate the legalization and authorization of medical cannabis, the number of cannabis extraction and analysis laboratories is increasing to meet the demand, requiring adequate analytical tools. Methodology: In response to numerous inquiries from physicians, analytical laboratories and users, the PROBIEN chromatography laboratory has selected two methods for the analysis of terpenes in Cannabis oil by gas chromatography technique (GC-FID). The methods are described using HP-5 and Innowax columns. The external standard method was used for the quantitative determination of β-Pinene, Myrcene, p-Cymene, Limonene, Linalool, α-Terpineol, Nerol and Geraniol. Results: good peak separation and reproducibility were observed, appropriate for the identification and quantification of the main terpenes in Cannabis extracts. The area/concentration ratio was linear in the range of 0.0005 to 2.0 mg/ml. Main conclusion: The described methods allow the identification and quantification of the major terpenes in Cannabis oil for an adequate quality control. Introducción: Cannabis sativa es una especie vegetal con gran número de principios activos, por lo que la lista de sus usos terapéuticos se está ampliando. En este sentido, hay numerosas evidencias del posible uso medicinal de los terpenos, así como del sinergismo de ellos con los cannabinoides (efecto séquito). Así, a medida que más países contemplan la legalización y autorización del cannabis medicinal, el número de laboratorios de extracción y análisis de cannabis aumenta para satisfacer la demanda, requiriéndose herramientas analíticas adecuadas. Metodología: En respuesta a numerosas consultas de médicos, laboratorios de análisis y usuarios, el laboratorio de cromatografía del PROBIEN ha seleccionado dos métodos para el análisis de terpenos en aceite de Cannabis por la técnica de cromatografía gaseosa (GC-FID). Se describen los métodos usando las columnas HP-5 e Innowax. Se empleó el método del estándar externo para la determinación cuantitativa de β-Pineno, Myrceno, p-Cymeno, Limoneno, Linalool, α-Terpineol, Nerol y Geraniol. Resultados: se observó una buena separación de picos y reproducibilidad, apropiadas para la identificación y cuantificación de los principales terpenos en extractos de Cannabis. La relación área/concentración fue lineal en el rango de 0,0005 a 2,0 mg/ml. Conclusión principal: los métodos descritos permiten la identificación y cuantificación de los terpenos mayoritarios en aceite de Cannabis para un control de calidad adecuado. Introdução: A Cannabis sativa é uma espécie vegetal com um grande número de princípios ativos, por isso a lista de seus usos terapêuticos está se expandindo. A este respeito, há amplas evidências do uso medicinal potencial dos terpenos, bem como de sua sinergia com os canabinoides (efeito de comitiva). Assim, à medida que mais países contemplam a legalização e autorização da canábis medicinal, o número de laboratórios de extração e teste de canábis está aumentando para atender à demanda, exigindo ferramentas analíticas apropriadas. Metodologia: Em resposta a numerosas consultas de médicos, laboratórios de análises e usuários, o laboratório de cromatografia PROBIEN selecionou dois métodos para a análise de terpenos em óleo de canábis pela técnica de cromatografia de gás (GC-FID). Os métodos são descritos usando as colunas HP-5 e Innowax. O método padrão externo foi utilizado para a determinação quantitativa de β-Pinene, Myrcene, p-Cymene, Limonene, Linalool, α-Terpineol, Nerol e Geraniol. Resultados: Foram observados bons picos de separação e reprodutibilidade, adequados para a identificação e quantificação dos principais terpenos em extratos de Canábis. A relação área/concentração foi linear na faixa de 0,0005 a 2,0 mg/ml. Conclusão principal: Os métodos descritos permitem a identificação e quantificação dos principais terpenos no óleo de Canábis para um controle de qualidade certo. Universidad Nacional Córdoba. Facultad de Ciencias Médicas. Secretaria de Ciencia y Tecnología 2023-06-30 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion texto texto texto text/html application/pdf https://revistas.unc.edu.ar/index.php/med/article/view/39593 10.31053/1853.0605.v80.n2.39593 Revista de la Facultad de Ciencias Médicas de Córdoba.; Vol. 80 No. 2 (2023); 99-105 Revista de la Facultad de Ciencias Médicas de Córdoba; Vol. 80 Núm. 2 (2023); 99-105 Revista da Faculdade de Ciências Médicas de Córdoba; v. 80 n. 2 (2023); 99-105 1853-0605 0014-6722 10.31053/1853.0605.v80.n2 spa https://revistas.unc.edu.ar/index.php/med/article/view/39593/41199 https://revistas.unc.edu.ar/index.php/med/article/view/39593/41201 Derechos de autor 2023 Universidad Nacional de Córdoba http://creativecommons.org/licenses/by-nc/4.0 |