Ghrelin and reproductive immunology: its association with the success of the assisted reproductive treatment
Assisted reproductive treatments (ART) usually include a controlled ovarian hyperstimulation (COH) protocol. This COH, increases supraphysiologically the levels of estradiol (E2) and progesterone (P4), and it might also alter the plasma concentrations of the immune-modulatory hormone...
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| Formato: | Artículo revista |
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Universidad Nacional Córdoba. Facultad de Ciencias Médicas. Secretaria de Ciencia y Tecnología
2022
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| Acceso en línea: | https://revistas.unc.edu.ar/index.php/med/article/view/39073 |
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I10-R327-article-39073 |
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| institution |
Universidad Nacional de Córdoba |
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I-10 |
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R-327 |
| container_title_str |
Revista de la Facultad de Ciencias Médicas de Córdoba |
| format |
Artículo revista |
| topic |
ghrelin controlled ovarian hyperstimulation reproductive immunology spontaneous abortions sexual steroids ghrelina hiperestimulación ovárica controlada inmunología reproductiva abortos espontáneos esteroides sexuales |
| spellingShingle |
ghrelin controlled ovarian hyperstimulation reproductive immunology spontaneous abortions sexual steroids ghrelina hiperestimulación ovárica controlada inmunología reproductiva abortos espontáneos esteroides sexuales Beltramone, F Estofán, P Piccinali, N Torres, PJ Ramírez, N Cantarelli, V Ponzio, MF Motrich, R Severgnini, M Martini, AC Ghrelin and reproductive immunology: its association with the success of the assisted reproductive treatment |
| topic_facet |
ghrelin controlled ovarian hyperstimulation reproductive immunology spontaneous abortions sexual steroids ghrelina hiperestimulación ovárica controlada inmunología reproductiva abortos espontáneos esteroides sexuales |
| author |
Beltramone, F Estofán, P Piccinali, N Torres, PJ Ramírez, N Cantarelli, V Ponzio, MF Motrich, R Severgnini, M Martini, AC |
| author_facet |
Beltramone, F Estofán, P Piccinali, N Torres, PJ Ramírez, N Cantarelli, V Ponzio, MF Motrich, R Severgnini, M Martini, AC |
| author_sort |
Beltramone, F |
| title |
Ghrelin and reproductive immunology: its association with the success of the assisted reproductive treatment |
| title_short |
Ghrelin and reproductive immunology: its association with the success of the assisted reproductive treatment |
| title_full |
Ghrelin and reproductive immunology: its association with the success of the assisted reproductive treatment |
| title_fullStr |
Ghrelin and reproductive immunology: its association with the success of the assisted reproductive treatment |
| title_full_unstemmed |
Ghrelin and reproductive immunology: its association with the success of the assisted reproductive treatment |
| title_sort |
ghrelin and reproductive immunology: its association with the success of the assisted reproductive treatment |
| description |
Assisted reproductive treatments (ART) usually include a controlled ovarian hyperstimulation (COH) protocol. This COH, increases supraphysiologically the levels of estradiol (E2) and progesterone (P4), and it might also alter the plasma concentrations of the immune-modulatory hormone ghrelin (Ghrl). These might exert deleterious effects on women´s uterine immune phenotype. The objective of this study was to evaluate if COH modifies ghrelinemia, and if this change impacts on ART success in general, and immune phenotype in particular.
After applying the inclusion and exclusion criteria, 97 female patients were recruited at the Centro Integral de Ginecología, Obstetricia y Reproducción (Córdoba). After signing the written informed consent, patients were divided according clinical criteria in one of three groups: 1) COH (n=35): women with COH and embryo transfer in the same cycle; 2) CET (n=25): women with criopreserved embryo transfer (i.e. in a cycle distinct to that of COH, with physiological levels of E2 and P4); and 3) OD (n=37): women with physiological levels of E2 and P4, but with embryos from donated oocytes. Data were analyzed by ANOVA or Chi-square test, with a significance level of 5%.
COH significantly increased Ghrl concentrations at embryo transfer (HOC:3684,2±1544,1pg/ml vs. TEC: 325,3±106,29pg/ml and OD:330,9±70,2pg/ml); also those of E2 and P4. COH showed also, significantly reduced indexes of spontaneous abortions (HOC:7,7% vs OD:28,6% and TEC:63,3%), and higher indexes of borne babies (HOC:92,3% vs.OD:71,4% and TEC:36,4%). Plasma levels of E2 and P4 from patients without spontaneous abortions were higher than those from women with spontaneous abortions (p<0.05). A similar profile was seen with Ghrl but without reaching significant differences; no women with a ghrelinemia higher than the Media suffered an abortion. The analysis of the subpopulations of peripheral blood lymphocytes at embryo transfer showed an association of COH with a reduced frequency of total T cells (HOC:29,4±2,5% vs. OD:38,6±1,2% and TEC:39,4±1,8%; p<0.05) and CD8 activated T cells (HOC:2,2±0,3% vs. OD:3,3±0,4 and TEC:2,7±0,4%; p<0.05).
These results indicate that COH might have a beneficial effect on ART, increasing Ghrl levels and the immune system modulation, and favoring embryo development/implantation and pregnancy success. |
| publisher |
Universidad Nacional Córdoba. Facultad de Ciencias Médicas. Secretaria de Ciencia y Tecnología |
| publishDate |
2022 |
| url |
https://revistas.unc.edu.ar/index.php/med/article/view/39073 |
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I10-R327-article-390732024-04-15T16:14:45Z Ghrelin and reproductive immunology: its association with the success of the assisted reproductive treatment Ghrelina, inmunología reproductiva y su asociación con el éxito del tratamiento de reproducción asistida Beltramone, F Estofán, P Piccinali, N Torres, PJ Ramírez, N Cantarelli, V Ponzio, MF Motrich, R Severgnini, M Martini, AC ghrelin controlled ovarian hyperstimulation reproductive immunology spontaneous abortions sexual steroids ghrelina hiperestimulación ovárica controlada inmunología reproductiva abortos espontáneos esteroides sexuales Assisted reproductive treatments (ART) usually include a controlled ovarian hyperstimulation (COH) protocol. This COH, increases supraphysiologically the levels of estradiol (E2) and progesterone (P4), and it might also alter the plasma concentrations of the immune-modulatory hormone ghrelin (Ghrl). These might exert deleterious effects on women´s uterine immune phenotype. The objective of this study was to evaluate if COH modifies ghrelinemia, and if this change impacts on ART success in general, and immune phenotype in particular. After applying the inclusion and exclusion criteria, 97 female patients were recruited at the Centro Integral de Ginecología, Obstetricia y Reproducción (Córdoba). After signing the written informed consent, patients were divided according clinical criteria in one of three groups: 1) COH (n=35): women with COH and embryo transfer in the same cycle; 2) CET (n=25): women with criopreserved embryo transfer (i.e. in a cycle distinct to that of COH, with physiological levels of E2 and P4); and 3) OD (n=37): women with physiological levels of E2 and P4, but with embryos from donated oocytes. Data were analyzed by ANOVA or Chi-square test, with a significance level of 5%. COH significantly increased Ghrl concentrations at embryo transfer (HOC:3684,2±1544,1pg/ml vs. TEC: 325,3±106,29pg/ml and OD:330,9±70,2pg/ml); also those of E2 and P4. COH showed also, significantly reduced indexes of spontaneous abortions (HOC:7,7% vs OD:28,6% and TEC:63,3%), and higher indexes of borne babies (HOC:92,3% vs.OD:71,4% and TEC:36,4%). Plasma levels of E2 and P4 from patients without spontaneous abortions were higher than those from women with spontaneous abortions (p<0.05). A similar profile was seen with Ghrl but without reaching significant differences; no women with a ghrelinemia higher than the Media suffered an abortion. The analysis of the subpopulations of peripheral blood lymphocytes at embryo transfer showed an association of COH with a reduced frequency of total T cells (HOC:29,4±2,5% vs. OD:38,6±1,2% and TEC:39,4±1,8%; p<0.05) and CD8 activated T cells (HOC:2,2±0,3% vs. OD:3,3±0,4 and TEC:2,7±0,4%; p<0.05). These results indicate that COH might have a beneficial effect on ART, increasing Ghrl levels and the immune system modulation, and favoring embryo development/implantation and pregnancy success. Los tratamientos de reproducción asistida (TRA) de alta complejidad, incluyen la hiperestimulación ovárica controlada (HOC). Ésta provoca concentraciones suprafisiológicas de estradiol (E2) y progesterona (P4), pudiendo además alterar los niveles de la hormona inmunomoduladora ghrelina (Ghrl), teniendo así posibles consecuencias sobre el fenotipo inmunológico uterino. El objetivo del estudio fue evaluar si la HOC modifica la ghrelinemia y si esto impacta sobre el éxito del TRA en general y del perfil inmunológico materno en particular. Tras aplicar los criterios de inclusión/exclusión, se reclutaron 97 pacientes del Centro Integral de Ginecología, Obstetricia y Reproducción de Córdoba que, luego de firmar consentimiento y de acuerdo a su clínica, fueron asignadas a uno de tres grupos: mujeres con hiperestimulación y transferencia embrionaria en el mismo ciclo, HOC (n=35); mujeres con transferencia embrionaria en un ciclo posterior con niveles fisiológicos de E2 y P4, TEC (n=25); y mujeres con niveles fisiológicos de E2 y P4 y embriones producto de ovodonación, OD (n=37). Los datos se analizaron mediante ANOVA o Chi-cuadrado y con un nivel de significancia del 5%. La HOC aumentó significativamente las concentraciones de Ghrl el día de la transferencia (HOC:3684,2±1544,1pg/ml vs. TEC: 325,3±106,29pg/ml y OD:330,9±70,2pg/ml); también los de E2 y P4. El grupo HOC mostró además tasas significativamente menores de abortos espontáneos (HOC:7,7% vs OD:28,6% y TEC:63,3%) y mayores de nacidos vivos (HOC:92,3% vs.OD:71,4% y TEC:36,4%). Los niveles de E2 y P4 en mujeres sin abortos espontáneos fueron significativamente mayores que los de las que sí los sufrieron. Una tendencia similar se observó con Ghrl: ninguna paciente con una ghrelinemia por encima de la media muestral sufrió abortos. El análisis de subpoblaciones linfocitarias en sangre periférica el día de la transferencia embrionaria reveló que la HOC se asoció a una disminución significativa en la frecuencia de linfocitos T totales (HOC:29,4±2,5% vs. OD:38,6±1,2% y TEC:39,4±1,8%) y T CD8 activados (HOC:2,2±0,3% vs. OD:3,3±0,4 y TEC:2,7±0,4%). Estos resultados indican que la HOC podría tener un efecto positivo en los TRA al elevar los niveles de Ghrl y así modular el sistema inmune materno, favoreciendo la implantación, el desarrollo embrionario y el éxito del embarazo. Universidad Nacional Córdoba. Facultad de Ciencias Médicas. Secretaria de Ciencia y Tecnología 2022-10-26 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion texto https://revistas.unc.edu.ar/index.php/med/article/view/39073 Revista de la Facultad de Ciencias Médicas de Córdoba.; Vol. 79 No. Suplemento JIC XXIII (2022): Suplemento JIC XXIII Revista de la Facultad de Ciencias Médicas de Córdoba; Vol. 79 Núm. Suplemento JIC XXIII (2022): Suplemento JIC XXIII Revista da Faculdade de Ciências Médicas de Córdoba; v. 79 n. Suplemento JIC XXIII (2022): Suplemento JIC XXIII 1853-0605 0014-6722 Derechos de autor 2022 Universidad Nacional de Córdoba http://creativecommons.org/licenses/by-nc/4.0 |