Retrospective detection of HHV-6 A and HHV-6B viruses in patients suspected of viral infection of the central nervous system in Córdoba, Argentina

In recent years, Herpesviruses HHV-6A and HHV-6B have been increasingly associated with infections of the central nervous system. Many investigations have reported these viruses as etiological agents of meningitis, encephalitis or meningoencephalitis in people of different ages and from different pa...

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Autores principales: Ferreyra, L, Biganzoli , P, Farias , A, Paván, J
Formato: Artículo revista
Publicado: Universidad Nacional Córdoba. Facultad de Ciencias Médicas. Secretaria de Ciencia y Tecnología 2022
Materias:
HHV-6A
HHV-6B
meningoencephalitis
Epidemiology
meningoencefalitis
epidemiologia
.
Acceso en línea:https://revistas.unc.edu.ar/index.php/med/article/view/38986
Aporte de:
Revista de la Facultad de Ciencias Médicas de Córdoba de Universidad Nacional de Córdoba
id I10-R327-article-38986
record_format ojs
institution Universidad Nacional de Córdoba
institution_str I-10
repository_str R-327
container_title_str Revista de la Facultad de Ciencias Médicas de Córdoba
format Artículo revista
topic HHV-6A
HHV-6B
meningoencephalitis
Epidemiology
HHV-6A
HHV-6B
meningoencefalitis
epidemiologia
.
spellingShingle HHV-6A
HHV-6B
meningoencephalitis
Epidemiology
HHV-6A
HHV-6B
meningoencefalitis
epidemiologia
.
Ferreyra, L
Biganzoli , P
Farias , A
Paván, J
Retrospective detection of HHV-6 A and HHV-6B viruses in patients suspected of viral infection of the central nervous system in Córdoba, Argentina
topic_facet HHV-6A
HHV-6B
meningoencephalitis
Epidemiology
HHV-6A
HHV-6B
meningoencefalitis
epidemiologia
.
author Ferreyra, L
Biganzoli , P
Farias , A
Paván, J
author_facet Ferreyra, L
Biganzoli , P
Farias , A
Paván, J
author_sort Ferreyra, L
title Retrospective detection of HHV-6 A and HHV-6B viruses in patients suspected of viral infection of the central nervous system in Córdoba, Argentina
title_short Retrospective detection of HHV-6 A and HHV-6B viruses in patients suspected of viral infection of the central nervous system in Córdoba, Argentina
title_full Retrospective detection of HHV-6 A and HHV-6B viruses in patients suspected of viral infection of the central nervous system in Córdoba, Argentina
title_fullStr Retrospective detection of HHV-6 A and HHV-6B viruses in patients suspected of viral infection of the central nervous system in Córdoba, Argentina
title_full_unstemmed Retrospective detection of HHV-6 A and HHV-6B viruses in patients suspected of viral infection of the central nervous system in Córdoba, Argentina
title_sort retrospective detection of hhv-6 a and hhv-6b viruses in patients suspected of viral infection of the central nervous system in córdoba, argentina
description In recent years, Herpesviruses HHV-6A and HHV-6B have been increasingly associated with infections of the central nervous system. Many investigations have reported these viruses as etiological agents of meningitis, encephalitis or meningoencephalitis in people of different ages and from different parts of the world. In our country, there is very little knowledge about the epidemiology of these viruses associated with infections of the central nervous system. Moreover, recent need to study the epidemiology of this viruses separately has arisen because until a few years ago they were considered variants of the same virus (HHV-6) and due to their biological, clinical and epidemiological differences they were reclassified as two different Herpesviruses. In order to make a contribution to the epidemiology of HHV-6A and B associated with central nervous system infections in our environment, we conducted a retrospective study in samples of cephalic spinal fluid (CSF) that were obtained in hospitalized patients suspected of having viral meningoencephalitis. For the study, 67 CSF samples obtained between January 2007 and March 2008 (age range 1-85 years) were selected. They were sent to the INVIV Institute of Virology for viral diagnosis and then preserved at -80°C. All selected samples were negative for other viruses such as Enterovirus, Herpes simplex, St. Louis encephalitis virus, West Nile virus and Dengue virus. For the detection of viral DNA, a generic nested PCR was used that detects both viruses (HHV-6). The samples that were positive were then typified as A or B through specific PCR techniques for each of the viruses. Of the 67 samples processed, 16 (23.9%) were positive for HHV-6, which, in turn, were all classified as HHV-6B. The present retrospective study allowed us to infer that HHV-6B is a frequent cause of meningoencephalitis in people of differen
publisher Universidad Nacional Córdoba. Facultad de Ciencias Médicas. Secretaria de Ciencia y Tecnología
publishDate 2022
url https://revistas.unc.edu.ar/index.php/med/article/view/38986
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spelling I10-R327-article-389862024-04-15T16:14:45Z Retrospective detection of HHV-6 A and HHV-6B viruses in patients suspected of viral infection of the central nervous system in Córdoba, Argentina Detección retrospectiva de los virus HHV-6 A y HHV-6B en pacientes sospechados de infección viral del sistema nervioso central en Córdoba, Argentina . Ferreyra, L Biganzoli , P Farias , A Paván, J HHV-6A HHV-6B meningoencephalitis Epidemiology HHV-6A HHV-6B meningoencefalitis epidemiologia . In recent years, Herpesviruses HHV-6A and HHV-6B have been increasingly associated with infections of the central nervous system. Many investigations have reported these viruses as etiological agents of meningitis, encephalitis or meningoencephalitis in people of different ages and from different parts of the world. In our country, there is very little knowledge about the epidemiology of these viruses associated with infections of the central nervous system. Moreover, recent need to study the epidemiology of this viruses separately has arisen because until a few years ago they were considered variants of the same virus (HHV-6) and due to their biological, clinical and epidemiological differences they were reclassified as two different Herpesviruses. In order to make a contribution to the epidemiology of HHV-6A and B associated with central nervous system infections in our environment, we conducted a retrospective study in samples of cephalic spinal fluid (CSF) that were obtained in hospitalized patients suspected of having viral meningoencephalitis. For the study, 67 CSF samples obtained between January 2007 and March 2008 (age range 1-85 years) were selected. They were sent to the INVIV Institute of Virology for viral diagnosis and then preserved at -80°C. All selected samples were negative for other viruses such as Enterovirus, Herpes simplex, St. Louis encephalitis virus, West Nile virus and Dengue virus. For the detection of viral DNA, a generic nested PCR was used that detects both viruses (HHV-6). The samples that were positive were then typified as A or B through specific PCR techniques for each of the viruses. Of the 67 samples processed, 16 (23.9%) were positive for HHV-6, which, in turn, were all classified as HHV-6B. The present retrospective study allowed us to infer that HHV-6B is a frequent cause of meningoencephalitis in people of differen En los años recientes los Herpesvirus HHV-6A y HHV-6B han sido asociados cada vez con mayor frecuencia a infecciones del sistema nervioso central. Muchas investigaciones han reportado casos de estos virus como agentes etiológicos de meningitis, encefalitis o meningoencefalitis en personas de diferentes edades y de muchas partes del mudo. En nuestro país, hay muy poco conocimiento sobre la epidemiología de estos virus asociados a infecciones del sistema nervioso central. Mas aún, recientemente ha surgido la necesidad de estudiar la epidemiología de estos virus por separado debido a que hasta hace pocos años eran considerados variantes de un mismo virus (HHV-6) y por sus diferencias biológicas, clínicas y epidemiológicas fueron reclasificados como dos  Herpesvirus distintos. Con el objetivo de realizar un aporte a la epidemiología del HHV-6A y B asociados a infecciones del sistema nervioso central en nuestro medio, realizamos un estudio retrospectivo en muestras de líquido céfalo raquídeo (LCR) que fueron obtenidos en pacientes internados sospechados de tener una meningoencefalitis viral. Para el estudio se seleccionaron 67 muestras de LCR obtenidas entre enero de 2007 y marzo de 2008 (rango de edad 1-85 años). Las mismas fueron enviadas al Instituto de Virología INVIV para diagnóstico viral y luego fueron conservadas a -80°C. Todas las muestras seleccionadas resultaron negativas para otros virus como Enterovirus, Herpes simplex, virus de la encefalitis de San Luis, virus del Nilo Occidental y virus Dengue. Para la detección del ADN viral se utilizó una nested PCR genérica que detecta ambos virus (HHV-6) y las muestras que resultaron positivas fueron luego tipificadas como A o B a través de técnicas de PCR específicas para cada uno de los virus. De las 67 muestras procesadas, 16 (23,9%) fueron positivas para HHV-6, las cuales, a su vez, resultaron todas tipificadas como HHV-6B. El presente estudio retrospectivo nos permitió inferir que el HHV-6B es una frecuente causa de meningoencefalitis en personas de diferentes edades de nuestro medio, recalcando a su vez la importancia de tener en cuenta a este virus en el diagnóstico de las infecciones del SNC. . Universidad Nacional Córdoba. Facultad de Ciencias Médicas. Secretaria de Ciencia y Tecnología 2022-10-26 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion texto texto . https://revistas.unc.edu.ar/index.php/med/article/view/38986 Revista de la Facultad de Ciencias Médicas de Córdoba.; Vol. 79 No. Suplemento JIC XXIII (2022): Suplemento JIC XXIII Revista de la Facultad de Ciencias Médicas de Córdoba; Vol. 79 Núm. Suplemento JIC XXIII (2022): Suplemento JIC XXIII Revista da Faculdade de Ciências Médicas de Córdoba; v. 79 n. Suplemento JIC XXIII (2022): Suplemento JIC XXIII 1853-0605 0014-6722 http://creativecommons.org/licenses/by-nc/4.0

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