Aspectos subcelulares de la inervación de las células intersticiales del ovario de embrión de pollo cultivado con LH O hCG
Severa¡ authors have shown the effect of LH or hCG gonadotrophins on steroid secrelion in the chick cmhryo ovary. However,the effects of luteinising horrnonc or human chorionic gonadotropin on chick emhryo ovary innervation have no[ heen studied. 1hc ptlrposc of the present work was to study the sub...
| Autores principales: | , , , |
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| Formato: | Artículo revista |
| Lenguaje: | Español |
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Universidad Nacional Córdoba. Facultad de Ciencias Médicas. Secretaria de Ciencia y Tecnología
2002
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| Materias: | |
| Acceso en línea: | https://revistas.unc.edu.ar/index.php/med/article/view/36776 |
| Aporte de: |
| id |
I10-R327-article-36776 |
|---|---|
| record_format |
ojs |
| institution |
Universidad Nacional de Córdoba |
| institution_str |
I-10 |
| repository_str |
R-327 |
| container_title_str |
Revista de la Facultad de Ciencias Médicas de Córdoba |
| language |
Español |
| format |
Artículo revista |
| topic |
Ulltrastructure Intertitial cell Innervation Chick emhryo ovary In vitro LH hCG Ultraestructura Células Intersticiales Inervación Ovario Embrión Pollo In vitro LH hCG |
| spellingShingle |
Ulltrastructure Intertitial cell Innervation Chick emhryo ovary In vitro LH hCG Ultraestructura Células Intersticiales Inervación Ovario Embrión Pollo In vitro LH hCG Ávila, Rodolfo Samar, María Ferraris, Ricardo Centurión, Claudio Aspectos subcelulares de la inervación de las células intersticiales del ovario de embrión de pollo cultivado con LH O hCG |
| topic_facet |
Ulltrastructure Intertitial cell Innervation Chick emhryo ovary In vitro LH hCG Ultraestructura Células Intersticiales Inervación Ovario Embrión Pollo In vitro LH hCG |
| author |
Ávila, Rodolfo Samar, María Ferraris, Ricardo Centurión, Claudio |
| author_facet |
Ávila, Rodolfo Samar, María Ferraris, Ricardo Centurión, Claudio |
| author_sort |
Ávila, Rodolfo |
| title |
Aspectos subcelulares de la inervación de las células intersticiales del ovario de embrión de pollo cultivado con LH O hCG |
| title_short |
Aspectos subcelulares de la inervación de las células intersticiales del ovario de embrión de pollo cultivado con LH O hCG |
| title_full |
Aspectos subcelulares de la inervación de las células intersticiales del ovario de embrión de pollo cultivado con LH O hCG |
| title_fullStr |
Aspectos subcelulares de la inervación de las células intersticiales del ovario de embrión de pollo cultivado con LH O hCG |
| title_full_unstemmed |
Aspectos subcelulares de la inervación de las células intersticiales del ovario de embrión de pollo cultivado con LH O hCG |
| title_sort |
aspectos subcelulares de la inervación de las células intersticiales del ovario de embrión de pollo cultivado con lh o hcg |
| description |
Severa¡ authors have shown the effect of LH or hCG gonadotrophins on steroid secrelion in the chick cmhryo ovary. However,the effects of luteinising horrnonc or human chorionic gonadotropin on chick emhryo ovary innervation have no[ heen studied. 1hc ptlrposc of the present work was to study the subcellular aspects of interstitial cells innervaliOn in chick enibryo ovaries cultured with LI-I or hCG Coobs White Rock female chick embryos at 7, 11 , 15 and 19 days of i ncubation (i n ovo) were employed. Explants of the left and right ovaries were cultured separately for 4 days in: 1- Basic medium (control): minimum essential medium(MEM-GIBCO) with 107 hovine fetal serum. 2- Basic medium with added LII or liCO (problems). The cultures were processed to bestudied ultrastructurally ('l'EM). Left and right ovary explants wore fixed two hours al room temperature in Karnovskys fixative. Thetissues were then post-fixed in osmiurn tetroxide i n 0. 1 NT cacodylate buffer for one hour and after a rinse in buffer were dehydrated in acetone; aftcrwards they were embedded in Araldite resin. Thick sections stained with 1 C/. Toluidine blue were examined and photographed using a Zeiss Photo II microscope. Thin sections were stained with uranyl acetate and lead citrate andexamined in a Siemens ElmisKop 101 electron microscope. Controls: In both ovaries the isolated interstitial cells and the appe:rance of nerve fibers and nerve endings were o!served in (he explants of 11 day cultured during four days and in a similar way at the age of 5 days of /o o vo clevelopmcnt. Problems : With\LH or hCG nerve fibers and nerve endings wer observed hoth in the right gonad and in the ¡nedulla of the left ovary in close cOntact with the steroid- producing interstitial ccl Is. Ultrastructurally, the interstitial cells are recognized by their typical oiganoids SER, Golgi apparatus. mitochondria with tubular crests and abundant lipidic droplets. The innervation of these cells were seen in ovaries at 7 days development, cultured during 4 days. The present state corresponds tothat of 11 days development in ovo. The nerve endings had mitochondria, microtubules and srnall and mediurn-sized vesicles, sorne with an electrodense content. These results jfl litro suggest that innervation of the ovaries is controlled by an indirect mechanism via the hypothalarnic-pituitary system and anoiher lo- cal with production neurotrophic factors modulated by LH. |
| publisher |
Universidad Nacional Córdoba. Facultad de Ciencias Médicas. Secretaria de Ciencia y Tecnología |
| publishDate |
2002 |
| url |
https://revistas.unc.edu.ar/index.php/med/article/view/36776 |
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2024-09-03T21:03:23Z |
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2024-09-03T21:03:23Z |
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1809210307719987200 |
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I10-R327-article-367762022-03-15T21:10:09Z Aspectos subcelulares de la inervación de las células intersticiales del ovario de embrión de pollo cultivado con LH O hCG Ávila, Rodolfo Samar, María Ferraris, Ricardo Centurión, Claudio Ulltrastructure Intertitial cell Innervation Chick emhryo ovary In vitro LH hCG Ultraestructura Células Intersticiales Inervación Ovario Embrión Pollo In vitro LH hCG Severa¡ authors have shown the effect of LH or hCG gonadotrophins on steroid secrelion in the chick cmhryo ovary. However,the effects of luteinising horrnonc or human chorionic gonadotropin on chick emhryo ovary innervation have no[ heen studied. 1hc ptlrposc of the present work was to study the subcellular aspects of interstitial cells innervaliOn in chick enibryo ovaries cultured with LI-I or hCG Coobs White Rock female chick embryos at 7, 11 , 15 and 19 days of i ncubation (i n ovo) were employed. Explants of the left and right ovaries were cultured separately for 4 days in: 1- Basic medium (control): minimum essential medium(MEM-GIBCO) with 107 hovine fetal serum. 2- Basic medium with added LII or liCO (problems). The cultures were processed to bestudied ultrastructurally ('l'EM). Left and right ovary explants wore fixed two hours al room temperature in Karnovskys fixative. Thetissues were then post-fixed in osmiurn tetroxide i n 0. 1 NT cacodylate buffer for one hour and after a rinse in buffer were dehydrated in acetone; aftcrwards they were embedded in Araldite resin. Thick sections stained with 1 C/. Toluidine blue were examined and photographed using a Zeiss Photo II microscope. Thin sections were stained with uranyl acetate and lead citrate andexamined in a Siemens ElmisKop 101 electron microscope. Controls: In both ovaries the isolated interstitial cells and the appe:rance of nerve fibers and nerve endings were o!served in (he explants of 11 day cultured during four days and in a similar way at the age of 5 days of /o o vo clevelopmcnt. Problems : With\LH or hCG nerve fibers and nerve endings wer observed hoth in the right gonad and in the ¡nedulla of the left ovary in close cOntact with the steroid- producing interstitial ccl Is. Ultrastructurally, the interstitial cells are recognized by their typical oiganoids SER, Golgi apparatus. mitochondria with tubular crests and abundant lipidic droplets. The innervation of these cells were seen in ovaries at 7 days development, cultured during 4 days. The present state corresponds tothat of 11 days development in ovo. The nerve endings had mitochondria, microtubules and srnall and mediurn-sized vesicles, sorne with an electrodense content. These results jfl litro suggest that innervation of the ovaries is controlled by an indirect mechanism via the hypothalarnic-pituitary system and anoiher lo- cal with production neurotrophic factors modulated by LH. Diversos autores han demostrado el efecto de LH y hCG sobre la secreción esteroidea del ovario del embrión de pollo. Sinembargo, no se ha investigado el efecto de las hormonas luteinizante y gonadotrofina coriónica humana sobre su inervación. El propósito del presente trabajo fue estudiar los aspectos subcelulares de la inervación de las células intersticiales de ovarios de embrión de pollo cultivados con LH o hCG. Explantos de ovarios izquierdo (funcionante) y derecho (regresivo) de 14 embriones de pollo de 7, 11, 15 y 19 días de desarrollo in ovo de raza Cohb's White Rock fueron cultivados por separado durante 4 días en : 1 Medio básico (control): niinimun cssential medium (MEM-GIBCO) con 10% de suero fetal bovino, 2- Medio básico con el agregado de LH o hCG (problemas). Los ovarios fueron seccionados en trozos de 2 a 3 mm de diámetro, lo que permitió realizar aproximadamente 30 cultivos de cada ovario. Controles: las características de las células intersticiales y la aparición de las fibras y terminaciones nerviosas observadas en los explantos de ambos ovarios 11 días cultivados durante cuatro días eran similares a los 15 días "iii ovo". Problemas: con LI-1 o hCG las fibras y terminaciones nerviosas fueron observadas en el ovario derecho y la médula del ovario izquierdo de 7 días cultivados durante cuatro días, en íntimo contacto con las células intersticiales productoras de esteroides. El pre- sente estadio se correspondía con los 11 días de desarrollo "in OVO" Estos hallazgos sugieren que la inervación de los ovarios estaría controlada por un mecanismo indirecto vía hipotálamo hipofisario y por otro local con la producción de factores neurotróficos modulada por LH. Universidad Nacional Córdoba. Facultad de Ciencias Médicas. Secretaria de Ciencia y Tecnología 2002-03-30 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion texto texto texto application/pdf https://revistas.unc.edu.ar/index.php/med/article/view/36776 10.31053/1853.0605.v59.n1.36776 Revista de la Facultad de Ciencias Médicas de Córdoba.; Vol. 59 No. 1 (2002); 63-69 Revista de la Facultad de Ciencias Médicas de Córdoba; Vol. 59 Núm. 1 (2002); 63-69 Revista da Faculdade de Ciências Médicas de Córdoba; v. 59 n. 1 (2002); 63-69 1853-0605 0014-6722 10.31053/1853.0605.v59.n1 spa https://revistas.unc.edu.ar/index.php/med/article/view/36776/37127 Derechos de autor 2022 Universidad Nacional de Córdoba http://creativecommons.org/licenses/by-nc/4.0 |