Suceptibility to infection and transmission of West Nile Virus (Flavivirus) by local Culex quinquefasciatus (Diptera: Culicidae)
West Nile Virus (WNV; Flavivirus; Flaviviridae) is a widely distributed arbovirus. It is maintained in an enzootic network by Culex mosquitoes and mainly passeriform birds. Despite its was first isolated in country in 2006, retrospective serological studies confirmed its circulatio...
| Autores principales: | , , , |
|---|---|
| Formato: | Artículo revista |
| Publicado: |
Universidad Nacional Córdoba. Facultad de Ciencias Médicas. Secretaria de Ciencia y Tecnología
2021
|
| Materias: | |
| Acceso en línea: | https://revistas.unc.edu.ar/index.php/med/article/view/36072 |
| Aporte de: |
| id |
I10-R327-article-36072 |
|---|---|
| record_format |
ojs |
| institution |
Universidad Nacional de Córdoba |
| institution_str |
I-10 |
| repository_str |
R-327 |
| container_title_str |
Revista de la Facultad de Ciencias Médicas de Córdoba |
| format |
Artículo revista |
| topic |
Arbovirus West Nile virus Vector Competence Culex quinquefasciatus Arbovirus virus West Nile Competencia Vectorial Culex quinquefasciatus . |
| spellingShingle |
Arbovirus West Nile virus Vector Competence Culex quinquefasciatus Arbovirus virus West Nile Competencia Vectorial Culex quinquefasciatus . Giayetto , O Beranek, MD Nazar, FN Díaz, LA Suceptibility to infection and transmission of West Nile Virus (Flavivirus) by local Culex quinquefasciatus (Diptera: Culicidae) |
| topic_facet |
Arbovirus West Nile virus Vector Competence Culex quinquefasciatus Arbovirus virus West Nile Competencia Vectorial Culex quinquefasciatus . |
| author |
Giayetto , O Beranek, MD Nazar, FN Díaz, LA |
| author_facet |
Giayetto , O Beranek, MD Nazar, FN Díaz, LA |
| author_sort |
Giayetto , O |
| title |
Suceptibility to infection and transmission of West Nile Virus (Flavivirus) by local Culex quinquefasciatus (Diptera: Culicidae) |
| title_short |
Suceptibility to infection and transmission of West Nile Virus (Flavivirus) by local Culex quinquefasciatus (Diptera: Culicidae) |
| title_full |
Suceptibility to infection and transmission of West Nile Virus (Flavivirus) by local Culex quinquefasciatus (Diptera: Culicidae) |
| title_fullStr |
Suceptibility to infection and transmission of West Nile Virus (Flavivirus) by local Culex quinquefasciatus (Diptera: Culicidae) |
| title_full_unstemmed |
Suceptibility to infection and transmission of West Nile Virus (Flavivirus) by local Culex quinquefasciatus (Diptera: Culicidae) |
| title_sort |
suceptibility to infection and transmission of west nile virus (flavivirus) by local culex quinquefasciatus (diptera: culicidae) |
| description |
West Nile Virus (WNV; Flavivirus; Flaviviridae) is a widely distributed arbovirus. It is maintained in an enzootic network by Culex mosquitoes and mainly passeriform birds. Despite its was first isolated in country in 2006, retrospective serological studies confirmed its circulation in Argentina by 2004. Since then, little has been elucidated regarding how WNV is maintained in nature and what species are involved in its amplification network. The purpose of this study was to evaluate the susceptibility to infection and transmission of a Cx. quinquefasciatus Say (Diptera: Culicidae) population from Córdoba, by means of a dose-response vector competence assay. A total of 110 mosquitoes were orally infected using 5 increasing viral loads (3,3; 3,7; 4,0; 6,0 y 7,0 log10 PFU/ml). After extrinsic incubation period, samples of abdomen, legs and salivary secretions were taken to evaluate infection, dissemination, and transmission rates. The evaluated population was susceptible to infection, showing infection rates ranging from 36 to 95%. Disseminated infections were detected at all viral doses, ranging from 50 to 100%. Finally, 38% (5/13) and 77% (17/22) of the mosquitoes with disseminated infections, corresponding to treatments 6 and 7 log10 PFU / ml, were capable of transmitting the virus. A linear regression was established between doses and transmission rates, and a minimum transmission threshold of 3.72 log10 PFU/ml was obtained. Taking into consideration our results in vector competence, their high abundance and even the lack of diapause of females during winter in Córdoba, we could expect this species to play a significant role in the maintenance and viral transmission throughout the year. Finally, the minimum transmission threshold suggests that Cx. quinquefasciatus could acquire WNV infection from local urban birds. |
| publisher |
Universidad Nacional Córdoba. Facultad de Ciencias Médicas. Secretaria de Ciencia y Tecnología |
| publishDate |
2021 |
| url |
https://revistas.unc.edu.ar/index.php/med/article/view/36072 |
| work_keys_str_mv |
AT giayettoo suceptibilitytoinfectionandtransmissionofwestnilevirusflavivirusbylocalculexquinquefasciatusdipteraculicidae AT beranekmd suceptibilitytoinfectionandtransmissionofwestnilevirusflavivirusbylocalculexquinquefasciatusdipteraculicidae AT nazarfn suceptibilitytoinfectionandtransmissionofwestnilevirusflavivirusbylocalculexquinquefasciatusdipteraculicidae AT diazla suceptibilitytoinfectionandtransmissionofwestnilevirusflavivirusbylocalculexquinquefasciatusdipteraculicidae AT giayettoo susceptibilidadalainfeccionytransmisiondelviruswestnileflavivirusenunapoblacionlocaldeculexquinquefasciatusdipteraculicidae AT beranekmd susceptibilidadalainfeccionytransmisiondelviruswestnileflavivirusenunapoblacionlocaldeculexquinquefasciatusdipteraculicidae AT nazarfn susceptibilidadalainfeccionytransmisiondelviruswestnileflavivirusenunapoblacionlocaldeculexquinquefasciatusdipteraculicidae AT diazla susceptibilidadalainfeccionytransmisiondelviruswestnileflavivirusenunapoblacionlocaldeculexquinquefasciatusdipteraculicidae |
| first_indexed |
2024-09-03T21:03:19Z |
| last_indexed |
2024-09-03T21:03:19Z |
| _version_ |
1809210303677726720 |
| spelling |
I10-R327-article-360722024-04-15T16:19:09Z Suceptibility to infection and transmission of West Nile Virus (Flavivirus) by local Culex quinquefasciatus (Diptera: Culicidae) Susceptibilidad a la infección y transmisión del virus West Nile (Flavivirus) en una población local de Culex quinquefasciatus (Diptera: Culicidae) . Giayetto , O Beranek, MD Nazar, FN Díaz, LA Arbovirus West Nile virus Vector Competence Culex quinquefasciatus Arbovirus virus West Nile Competencia Vectorial Culex quinquefasciatus . West Nile Virus (WNV; Flavivirus; Flaviviridae) is a widely distributed arbovirus. It is maintained in an enzootic network by Culex mosquitoes and mainly passeriform birds. Despite its was first isolated in country in 2006, retrospective serological studies confirmed its circulation in Argentina by 2004. Since then, little has been elucidated regarding how WNV is maintained in nature and what species are involved in its amplification network. The purpose of this study was to evaluate the susceptibility to infection and transmission of a Cx. quinquefasciatus Say (Diptera: Culicidae) population from Córdoba, by means of a dose-response vector competence assay. A total of 110 mosquitoes were orally infected using 5 increasing viral loads (3,3; 3,7; 4,0; 6,0 y 7,0 log10 PFU/ml). After extrinsic incubation period, samples of abdomen, legs and salivary secretions were taken to evaluate infection, dissemination, and transmission rates. The evaluated population was susceptible to infection, showing infection rates ranging from 36 to 95%. Disseminated infections were detected at all viral doses, ranging from 50 to 100%. Finally, 38% (5/13) and 77% (17/22) of the mosquitoes with disseminated infections, corresponding to treatments 6 and 7 log10 PFU / ml, were capable of transmitting the virus. A linear regression was established between doses and transmission rates, and a minimum transmission threshold of 3.72 log10 PFU/ml was obtained. Taking into consideration our results in vector competence, their high abundance and even the lack of diapause of females during winter in Córdoba, we could expect this species to play a significant role in the maintenance and viral transmission throughout the year. Finally, the minimum transmission threshold suggests that Cx. quinquefasciatus could acquire WNV infection from local urban birds. El virus West Nile (VWN) (Flavivirus; Flaviviridae) es un arbovirus ampliamente distribuido en el mundo y mantenido de manera enzoótica por mosquitos del género Culex y aves paseriformes. A pesar de que fue aislado por primera vez en nuestro país en el 2006, estudios serológicos retrospectivos confirmaron que circulaba de manera autóctona desde fines del año 2004. Desde entonces, poco se ha podido dilucidar cómo este virus es mantenido en la naturaleza y que especies de vectores y hospedadores se encuentran involucrados en su red de mantenimiento y amplificación. Este trabajo evaluó la susceptibilidad a la infección y transmisión de una población de Cx. quinquefasciatus Say (Diptera: Culicidae) de la ciudad de Córdoba por medio de un ensayo de dosis-respuesta de competencia vectorial para el VWN. Un total de 110 mosquitos fueron infectados vía oral con 5 cargas virales crecientes de virus (3,3; 3,7; 4,0; 6,0 y 7,0 log10 UFP/mL). Tras el periodo de incubación extrínseco, se tomaron muestras de abdomen, patas y saliva para evaluar los índices de infección, diseminación y transmisión viral. La población evaluada fue susceptible a la infección oral por el VWN detectándose porcentajes de infección entre 36% y el 95%. Se detectaron infecciones diseminadas a todas las dosis virales, variando entre un 50 y un 100% entre la menor y mayor dosis. Un 38% (5/13) y un 77% (17/22) de los mosquitos con infecciones diseminadas, correspondientes a los tratamientos 6 y 7 log10 UFP/ml, fueron capaces de transmitir el virus. A partir de estos resultados se estableció una relación lineal entre la dosis y las tasas de transmisión y se calculó un umbral mínimo de transmisión de 3,72 UFP/ml. Considerando los resultados en cuanto a la competencia de Cx. quinquefasciatus como potencial vector del VWN, su elevada abundancia e incluso la falta de diapausa de las hembras durante el invierno en Córdoba, podríamos esperar que esta especie juegue un papel significativo en el mantenimiento y transmisión viral a lo largo de todo el año. Finalmente, el umbral mínimo de transmisión sugiere que Cx. quinquefasciatus podría adquirir la infección por VWN de aves urbanas locales. . Universidad Nacional Córdoba. Facultad de Ciencias Médicas. Secretaria de Ciencia y Tecnología 2021-10-07 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion texto texto texto https://revistas.unc.edu.ar/index.php/med/article/view/36072 Revista de la Facultad de Ciencias Médicas de Córdoba.; Vol. 78 No. Suplemento (2021): Suplemento JIC XXII Revista de la Facultad de Ciencias Médicas de Córdoba; Vol. 78 Núm. Suplemento (2021): Suplemento JIC XXII Revista da Faculdade de Ciências Médicas de Córdoba; v. 78 n. Suplemento (2021): Suplemento JIC XXII 1853-0605 0014-6722 http://creativecommons.org/licenses/by-nc/4.0 |