Virulence evaluation of the Argentinean Eq7229 West Nile Virus strain in C57BL/6 mice
West Nile virus (WNV, Flaviviridae, Flavivirus) is a mosquito-borne virus amplified by a wide variety of bird species. In the last decades it has been recognized as an etiological agent of veterinary and public health concern, causing encephalitis outbreaks in birds, horses and humans. Therefore, st...
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| Formato: | Artículo revista |
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Universidad Nacional Córdoba. Facultad de Ciencias Médicas. Secretaria de Ciencia y Tecnología
2021
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| Acceso en línea: | https://revistas.unc.edu.ar/index.php/med/article/view/36065 |
| Aporte de: |
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I10-R327-article-36065 |
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ojs |
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Universidad Nacional de Córdoba |
| institution_str |
I-10 |
| repository_str |
R-327 |
| container_title_str |
Revista de la Facultad de Ciencias Médicas de Córdoba |
| format |
Artículo revista |
| topic |
West Nile virus Arbovirus Virulence animal model virus West Nile Arbovirus Virulencia modelo animal . |
| spellingShingle |
West Nile virus Arbovirus Virulence animal model virus West Nile Arbovirus Virulencia modelo animal . Giayetto , O Nazar, FN Díaz, LA Virulence evaluation of the Argentinean Eq7229 West Nile Virus strain in C57BL/6 mice |
| topic_facet |
West Nile virus Arbovirus Virulence animal model virus West Nile Arbovirus Virulencia modelo animal . |
| author |
Giayetto , O Nazar, FN Díaz, LA |
| author_facet |
Giayetto , O Nazar, FN Díaz, LA |
| author_sort |
Giayetto , O |
| title |
Virulence evaluation of the Argentinean Eq7229 West Nile Virus strain in C57BL/6 mice |
| title_short |
Virulence evaluation of the Argentinean Eq7229 West Nile Virus strain in C57BL/6 mice |
| title_full |
Virulence evaluation of the Argentinean Eq7229 West Nile Virus strain in C57BL/6 mice |
| title_fullStr |
Virulence evaluation of the Argentinean Eq7229 West Nile Virus strain in C57BL/6 mice |
| title_full_unstemmed |
Virulence evaluation of the Argentinean Eq7229 West Nile Virus strain in C57BL/6 mice |
| title_sort |
virulence evaluation of the argentinean eq7229 west nile virus strain in c57bl/6 mice |
| description |
West Nile virus (WNV, Flaviviridae, Flavivirus) is a mosquito-borne virus amplified by a wide variety of bird species. In the last decades it has been recognized as an etiological agent of veterinary and public health concern, causing encephalitis outbreaks in birds, horses and humans. Therefore, studies focused to characterize its biological features are important. In Argentina, WNV has been circulated since 2004, but aspects regarding its ecology and virulence of autochthonous strain are still unknown. The purpose of this study was to characterize the virulence of the WNV strain Eq7229, isolated in Argentina. Three, six and thirteen weeks old C57BL/6 mice were intraperitoneally inoculated with increasing doses ranging from 10 to 105 PFU suspended in 100µl of cell culture medium. For the thirteen-week-old adults, only the two higher doses were administrated. A total of 8 mice per treatment were used. Groups of six mice per age were inoculated with cell culture medium and maintained as control groups. Mortality rates were obtained and used to calculate LD50 using the Reed-Muench method. Survival curves for each age were calculated using the Kaplan-Mayer test. The experiment was approved by the ethical committee at Instituto de Investigaciones Biológicas y Tecnológicas (Acta nº 13, 20/08/2020). For the three-weeks-old mice, the calculated LD50 was <10 UFP, for the six-week-old mice 50,11 PFU, and for the thirteen-week-old mice was >105 PFU. No significative differences were observed regarding survival curves for the three and six-week-old mice (log rank, p= 0.46; p= 0.095 respectively). Thirteen-week-old mice showed no mortality nor morbidity to WNV exposure. These results show a high virulence of the strain under study. In addition, from these results we will be able to establish the initial parameters to deepen the study of this strain in the murine model. |
| publisher |
Universidad Nacional Córdoba. Facultad de Ciencias Médicas. Secretaria de Ciencia y Tecnología |
| publishDate |
2021 |
| url |
https://revistas.unc.edu.ar/index.php/med/article/view/36065 |
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2024-09-03T21:03:19Z |
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I10-R327-article-360652024-04-15T16:19:09Z Virulence evaluation of the Argentinean Eq7229 West Nile Virus strain in C57BL/6 mice Evaluación de la virulencia de la cepa Eq7229 del virus West Nile en ratones C57BL/6 . Giayetto , O Nazar, FN Díaz, LA West Nile virus Arbovirus Virulence animal model virus West Nile Arbovirus Virulencia modelo animal . West Nile virus (WNV, Flaviviridae, Flavivirus) is a mosquito-borne virus amplified by a wide variety of bird species. In the last decades it has been recognized as an etiological agent of veterinary and public health concern, causing encephalitis outbreaks in birds, horses and humans. Therefore, studies focused to characterize its biological features are important. In Argentina, WNV has been circulated since 2004, but aspects regarding its ecology and virulence of autochthonous strain are still unknown. The purpose of this study was to characterize the virulence of the WNV strain Eq7229, isolated in Argentina. Three, six and thirteen weeks old C57BL/6 mice were intraperitoneally inoculated with increasing doses ranging from 10 to 105 PFU suspended in 100µl of cell culture medium. For the thirteen-week-old adults, only the two higher doses were administrated. A total of 8 mice per treatment were used. Groups of six mice per age were inoculated with cell culture medium and maintained as control groups. Mortality rates were obtained and used to calculate LD50 using the Reed-Muench method. Survival curves for each age were calculated using the Kaplan-Mayer test. The experiment was approved by the ethical committee at Instituto de Investigaciones Biológicas y Tecnológicas (Acta nº 13, 20/08/2020). For the three-weeks-old mice, the calculated LD50 was <10 UFP, for the six-week-old mice 50,11 PFU, and for the thirteen-week-old mice was >105 PFU. No significative differences were observed regarding survival curves for the three and six-week-old mice (log rank, p= 0.46; p= 0.095 respectively). Thirteen-week-old mice showed no mortality nor morbidity to WNV exposure. These results show a high virulence of the strain under study. In addition, from these results we will be able to establish the initial parameters to deepen the study of this strain in the murine model. El virus West Nile (VWN) es un flavivirus transmitido por mosquitos y amplificado por aves. En los últimos años, se lo ha reconocido como un importante agente etiológico a nivel mundial de encefalitis en aves, equinos y humanos, por lo que reviste importancia las actividades relacionadas a su aislamiento y caracterización. En Argentina su circulación autóctona data desde el 2004. Su ecología y caracterización biológica de cepas está prácticamente desconocida. El propósito de este trabajo fue caracterizar la virulencia de la cepa Eq7229 de VWN, que se aisló en Argentina en 2006. Para ello se utilizaron tres modelos etarios (21, 42 y 91 días de edad, d.e) de ratones C57BL/6 a los que se les inocularon dosis crecientes de 10 a 105 UFP administradas en 100µl de MEM vía intraperitoneal. En el modelo de 91 d.e, solo se utilizaron las dos dosis más elevadas. Un total de 8 ratones por tratamiento fueron utilizados en el experimento, además de 6 ratones por modelo etario que se utilizaron como control. Se calcularon porcentajes de mortalidad y morbilidad, la dosis letal 50 (DL50) por el método de Reed-Muench y se analizaron las curvas de supervivencia por grupo etario utilizando el test de Kaplan-Meier. El experimento fue aprobado por la comisión del CICUAL del IIBYT - CONICET (Acta nº 13, 20/08/2020). Las DL50 calculadas para los distintos modelos etarios fueron <10 UFP para los ratones de 21 d.e, 50,11 UFP para los ratones de 42 d.e y >105 para los de 91 d.e. No se encontraron diferencias significativas en la supervivencia entre las dosis virales en ratones de 21 d.e (log rank, p= 0,46) ni en los de 42 d.e (p= 0,095). Los ratones de 91 d.e no mostraron mortalidad ante la exposición al VWN. Estos resultados muestran una alta virulencia de la cepa en estudio. Además, a partir de estos resultados podremos establecer los parámetros iniciales para profundizar el estudio de esta cepa en el modelo murino. . Universidad Nacional Córdoba. Facultad de Ciencias Médicas. Secretaria de Ciencia y Tecnología 2021-10-07 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion texto texto texto https://revistas.unc.edu.ar/index.php/med/article/view/36065 Revista de la Facultad de Ciencias Médicas de Córdoba.; Vol. 78 No. Suplemento (2021): Suplemento JIC XXII Revista de la Facultad de Ciencias Médicas de Córdoba; Vol. 78 Núm. Suplemento (2021): Suplemento JIC XXII Revista da Faculdade de Ciências Médicas de Córdoba; v. 78 n. Suplemento (2021): Suplemento JIC XXII 1853-0605 0014-6722 http://creativecommons.org/licenses/by-nc/4.0 |