Molecular detection of cytomegalovirus in serum of pregnant women and newborns with pathology compatible with infection during pregnancy in Córdoba, Argentina
Abstract: Cytomegalovirus (CMV) is a cause of congenital infection (0,2%-6,1% of newborns worldwide) and is the main infectious etiology of congenital anomalies, with significant socioeconomic impact due to the hearing loss and the neurodevelopmental impairment. However, epidemiological da...
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| Formato: | Artículo revista |
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Universidad Nacional Córdoba. Facultad de Ciencias Médicas. Secretaria de Ciencia y Tecnología
2021
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| Acceso en línea: | https://revistas.unc.edu.ar/index.php/med/article/view/35028 |
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I10-R327-article-35028 |
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ojs |
| institution |
Universidad Nacional de Córdoba |
| institution_str |
I-10 |
| repository_str |
R-327 |
| container_title_str |
Revista de la Facultad de Ciencias Médicas de Córdoba |
| format |
Artículo revista |
| topic |
cytomegalovirus congenital PREGNANCY newborn citomegalovirus congénito EMBARAZO recién nacido |
| spellingShingle |
cytomegalovirus congenital PREGNANCY newborn citomegalovirus congénito EMBARAZO recién nacido Olivera , NL Colazo Salbetti , MB Tenaglia , M Hernández , G Alfaro , J Riberi , I Isa , MB Bertoldi , A Pedranti , M Adamo , MP Molecular detection of cytomegalovirus in serum of pregnant women and newborns with pathology compatible with infection during pregnancy in Córdoba, Argentina |
| topic_facet |
cytomegalovirus congenital PREGNANCY newborn citomegalovirus congénito EMBARAZO recién nacido |
| author |
Olivera , NL Colazo Salbetti , MB Tenaglia , M Hernández , G Alfaro , J Riberi , I Isa , MB Bertoldi , A Pedranti , M Adamo , MP |
| author_facet |
Olivera , NL Colazo Salbetti , MB Tenaglia , M Hernández , G Alfaro , J Riberi , I Isa , MB Bertoldi , A Pedranti , M Adamo , MP |
| author_sort |
Olivera , NL |
| title |
Molecular detection of cytomegalovirus in serum of pregnant women and newborns with pathology compatible with infection during pregnancy in Córdoba, Argentina |
| title_short |
Molecular detection of cytomegalovirus in serum of pregnant women and newborns with pathology compatible with infection during pregnancy in Córdoba, Argentina |
| title_full |
Molecular detection of cytomegalovirus in serum of pregnant women and newborns with pathology compatible with infection during pregnancy in Córdoba, Argentina |
| title_fullStr |
Molecular detection of cytomegalovirus in serum of pregnant women and newborns with pathology compatible with infection during pregnancy in Córdoba, Argentina |
| title_full_unstemmed |
Molecular detection of cytomegalovirus in serum of pregnant women and newborns with pathology compatible with infection during pregnancy in Córdoba, Argentina |
| title_sort |
molecular detection of cytomegalovirus in serum of pregnant women and newborns with pathology compatible with infection during pregnancy in córdoba, argentina |
| description |
Abstract:
Cytomegalovirus (CMV) is a cause of congenital infection (0,2%-6,1% of newborns worldwide) and is the main infectious etiology of congenital anomalies, with significant socioeconomic impact due to the hearing loss and the neurodevelopmental impairment. However, epidemiological data in our city are scarce. In order to contribute to characterize the local epidemiological scenario of the CMV congenital infection, the objective of this work was to determine the frequency of detection of viral DNA in serum of patients with pathologies that can be associated with infection during pregnancy.
The protocol was approved by the Ethics Committee (CIEIS) of Clínica Universitaria Reina Fabiola and there was no conflict of interest. A PCR technique for detection of CMV was implemented and DNA extracts were retrospectively analyzed. One hundred and seven serum samples stored in the biobank, obtained between 2014 and 2021 by referrals for diagnosis and in the context of previous studies, were included. The study sample was composed of two subgroups of patients: one included 76 pregnant patients aged 16 to 45 years old, who presented one or more clinical signs that suggested the possibility of CMV infection; the other subgroup enclosed 31 newborns and children up to 10 months of age with TORCH syndrome.
Out of the total of 107 samples, CMV DNA was detected in 4 (3,73%) patients. Among pregnant patients, CMV was detected in 2/76 (2,56%) patients. The clinical manifestations in one of them were anemia and leukorrhea followed by fetal death. In the second patient, there were no symptoms described on the records, apart from the presumption of TORCH by the treating physician. However, in this patient, a subsequent diagnosis of Trisomy 21 was also reported. Among the newborns and children, CMV viral genome was detected in 2/31 (6.45%) patients, both with initial presumption of congenital rubella (ruled out by the laboratory of the surveillance network). One of them presented corneal leukoma, while in the other there was no specific clinical signs recorded.
The results obtained indicate the need of deeper studies about gestational and congenital CMV infection in our setting, with complete medical records. |
| publisher |
Universidad Nacional Córdoba. Facultad de Ciencias Médicas. Secretaria de Ciencia y Tecnología |
| publishDate |
2021 |
| url |
https://revistas.unc.edu.ar/index.php/med/article/view/35028 |
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I10-R327-article-350282024-04-15T16:19:09Z Molecular detection of cytomegalovirus in serum of pregnant women and newborns with pathology compatible with infection during pregnancy in Córdoba, Argentina Detección molecular de citomegalovirus en suero de embarazadas y recién nacidos con patología compatible con infección durante la gestación en Córdoba, Argentina Olivera , NL Colazo Salbetti , MB Tenaglia , M Hernández , G Alfaro , J Riberi , I Isa , MB Bertoldi , A Pedranti , M Adamo , MP cytomegalovirus congenital PREGNANCY newborn citomegalovirus congénito EMBARAZO recién nacido Abstract: Cytomegalovirus (CMV) is a cause of congenital infection (0,2%-6,1% of newborns worldwide) and is the main infectious etiology of congenital anomalies, with significant socioeconomic impact due to the hearing loss and the neurodevelopmental impairment. However, epidemiological data in our city are scarce. In order to contribute to characterize the local epidemiological scenario of the CMV congenital infection, the objective of this work was to determine the frequency of detection of viral DNA in serum of patients with pathologies that can be associated with infection during pregnancy. The protocol was approved by the Ethics Committee (CIEIS) of Clínica Universitaria Reina Fabiola and there was no conflict of interest. A PCR technique for detection of CMV was implemented and DNA extracts were retrospectively analyzed. One hundred and seven serum samples stored in the biobank, obtained between 2014 and 2021 by referrals for diagnosis and in the context of previous studies, were included. The study sample was composed of two subgroups of patients: one included 76 pregnant patients aged 16 to 45 years old, who presented one or more clinical signs that suggested the possibility of CMV infection; the other subgroup enclosed 31 newborns and children up to 10 months of age with TORCH syndrome. Out of the total of 107 samples, CMV DNA was detected in 4 (3,73%) patients. Among pregnant patients, CMV was detected in 2/76 (2,56%) patients. The clinical manifestations in one of them were anemia and leukorrhea followed by fetal death. In the second patient, there were no symptoms described on the records, apart from the presumption of TORCH by the treating physician. However, in this patient, a subsequent diagnosis of Trisomy 21 was also reported. Among the newborns and children, CMV viral genome was detected in 2/31 (6.45%) patients, both with initial presumption of congenital rubella (ruled out by the laboratory of the surveillance network). One of them presented corneal leukoma, while in the other there was no specific clinical signs recorded. The results obtained indicate the need of deeper studies about gestational and congenital CMV infection in our setting, with complete medical records. Resumen: El citomegalovirus (CMV) causa infección congénita (0,2%-6,1% de los nacidos vivos en todo el mundo) y es la principal etiología infecciosa de anomalías congénitas, con importante impacto socioeconómico por la pérdida de audición en la niñez y el retraso del neurodesarrollo. Sin embargo, en nuestro medio los datos son escasos. A fin de contribuir a caracterizar el escenario epidemiológico local de la infección congénita por CMV, el objetivo de este trabajo fue determinar la frecuencia de detección de ADN viral en suero de pacientes con patología compatible con la infección durante la gestación. El estudio fue aprobado por el CIEIS Reina Fabiola y no hubo conflicto de intereses. Se implementó una técnica de PCR para detección de CMV y se analizaron retrospectivamente extractos de ADN de 107 muestras de suero almacenados en biobanco, obtenidas por derivaciones para diagnóstico y en el contexto de estudios previos entre 2014 y 2021. La muestra estudiada incluyó dos subgrupos: uno integrado por 76 pacientes embarazadas de 16 a 45 años de edad con uno o más signos clínicos que sugerían la posibilidad de infección por CMV; y el otro, constituido por 31 neonatos y niños de hasta 10 meses de edad con síndrome TORCH. En el total de 107 muestras se detectó ADN de CMV en 4 (3,73%). Entre las pacientes embarazadas, se detectó en 2/76 (2,56%). Las manifestaciones clínicas en una paciente fueron anemia y leucorrea seguidas de muerte fetal. En la segunda paciente no hay clínica descrita en los registros, aparte de la sospecha de TORCH del médico tratante. En esta última paciente se informó además un diagnóstico posterior de trisomía 21. Entre los neonatos y niños se detectó genoma viral en 2/31 (6,45%), ambos con sospecha inicial de rubéola congénita (descartada por el laboratorio de la red de vigilancia). Uno de ellos presentó leucoma corneal, mientras que del otro no hay registro de signos clínicos específicos. Los resultados obtenidos indican la necesidad de profundizar en el estudio de la infección gestacional y congénita por CMV en nuestro medio, con historias clínicas completas. Universidad Nacional Córdoba. Facultad de Ciencias Médicas. Secretaria de Ciencia y Tecnología 2021-10-12 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion texto texto texto https://revistas.unc.edu.ar/index.php/med/article/view/35028 Revista de la Facultad de Ciencias Médicas de Córdoba.; Vol. 78 No. Suplemento (2021): Suplemento JIC XXII Revista de la Facultad de Ciencias Médicas de Córdoba; Vol. 78 Núm. Suplemento (2021): Suplemento JIC XXII Revista da Faculdade de Ciências Médicas de Córdoba; v. 78 n. Suplemento (2021): Suplemento JIC XXII 1853-0605 0014-6722 http://creativecommons.org/licenses/by-nc/4.0 |