Perinatal exposure to the phthalate "DEHP" alters the expression of estrogen receptors on somatotroph cells, increasing their proliferation

Abstract:  Phthalates are chemicals widely used to make soft and flexible plastics. Di-2-ethylhexyl phthalate (DEHP) is the most commonly used, with high human exposure occurring from fetal development, acting as an endocrine disruptor by interfering with hormonal signaling.&nb...

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Detalles Bibliográficos
Autores principales: Pérez , PA, Toledo , J, Picech , F, Petiti , JP, Mukdsi , JH, Torres , AL, De Paul , AL, Gutierrez , S
Formato: Artículo revista
Publicado: Universidad Nacional Córdoba. Facultad de Ciencias Médicas. Secretaria de Ciencia y Tecnología 2021
Materias:
DI- (2-ETHYLHEXYL PHTHALATE) (DEHP)
SOMATOTROPHS
ALPHA AND BETA ESTROGEN RECEPTORS
CELL PROLIFERATION
DI-(2-ETHYLHEXYL FTALATO) (DEHP)
SOMATOTROPAS
RECEPTORES ESTROGENICOS ALFA Y BETA
PROLIFERACION CELULAR
Acceso en línea:https://revistas.unc.edu.ar/index.php/med/article/view/35024
Aporte de:
Revista de la Facultad de Ciencias Médicas de Córdoba de Universidad Nacional de Córdoba
id I10-R327-article-35024
record_format ojs
institution Universidad Nacional de Córdoba
institution_str I-10
repository_str R-327
container_title_str Revista de la Facultad de Ciencias Médicas de Córdoba
format Artículo revista
topic DI- (2-ETHYLHEXYL PHTHALATE) (DEHP)
SOMATOTROPHS
ALPHA AND BETA ESTROGEN RECEPTORS
CELL PROLIFERATION
DI-(2-ETHYLHEXYL FTALATO) (DEHP)
SOMATOTROPAS
RECEPTORES ESTROGENICOS ALFA Y BETA
PROLIFERACION CELULAR
spellingShingle DI- (2-ETHYLHEXYL PHTHALATE) (DEHP)
SOMATOTROPHS
ALPHA AND BETA ESTROGEN RECEPTORS
CELL PROLIFERATION
DI-(2-ETHYLHEXYL FTALATO) (DEHP)
SOMATOTROPAS
RECEPTORES ESTROGENICOS ALFA Y BETA
PROLIFERACION CELULAR
Pérez , PA
Toledo , J
Picech , F
Petiti , JP
Mukdsi , JH
Torres , AL
De Paul , AL
Gutierrez , S
Perinatal exposure to the phthalate "DEHP" alters the expression of estrogen receptors on somatotroph cells, increasing their proliferation
topic_facet DI- (2-ETHYLHEXYL PHTHALATE) (DEHP)
SOMATOTROPHS
ALPHA AND BETA ESTROGEN RECEPTORS
CELL PROLIFERATION
DI-(2-ETHYLHEXYL FTALATO) (DEHP)
SOMATOTROPAS
RECEPTORES ESTROGENICOS ALFA Y BETA
PROLIFERACION CELULAR
author Pérez , PA
Toledo , J
Picech , F
Petiti , JP
Mukdsi , JH
Torres , AL
De Paul , AL
Gutierrez , S
author_facet Pérez , PA
Toledo , J
Picech , F
Petiti , JP
Mukdsi , JH
Torres , AL
De Paul , AL
Gutierrez , S
author_sort Pérez , PA
title Perinatal exposure to the phthalate "DEHP" alters the expression of estrogen receptors on somatotroph cells, increasing their proliferation
title_short Perinatal exposure to the phthalate "DEHP" alters the expression of estrogen receptors on somatotroph cells, increasing their proliferation
title_full Perinatal exposure to the phthalate "DEHP" alters the expression of estrogen receptors on somatotroph cells, increasing their proliferation
title_fullStr Perinatal exposure to the phthalate "DEHP" alters the expression of estrogen receptors on somatotroph cells, increasing their proliferation
title_full_unstemmed Perinatal exposure to the phthalate "DEHP" alters the expression of estrogen receptors on somatotroph cells, increasing their proliferation
title_sort perinatal exposure to the phthalate "dehp" alters the expression of estrogen receptors on somatotroph cells, increasing their proliferation
description Abstract:  Phthalates are chemicals widely used to make soft and flexible plastics. Di-2-ethylhexyl phthalate (DEHP) is the most commonly used, with high human exposure occurring from fetal development, acting as an endocrine disruptor by interfering with hormonal signaling. The objective of this study was to analyze the effect of perinatal exposure to DEHP on pituitary Estrogen receptor (ER)α and β expression and its impact on somatotroph cells.  For this, 10 pregnant Wistar rats were exposed by gavage to DEHP (200 µg / kg) or corn oil from day 0 of gestation, during pregnancy and lactation. For In vivo experiments, glands from 30 75-day-old male pups were used. For in vitro experiments, 3 pituitary cultures from 10 male rats were stimulated with 1, 10 or 100nM DEHP for 72 h. The percentages of somatotroph cells expressing ERα and β were quantified in pituitary cryosections by double immunostaining using confocal microscopy. Cell proliferation was quantified by double immunostaining (ki67+ GH+). The somatotroph cell population was quantified in vivo and in vitro by flow cytometry and immunocytochemistry, respectively. Statistical analysis: ANOVA Tukey (P <0.05). Our results showed that perinatal exposure to DEHP significantly increased the percentage of somatotrophs that expressed ERα (GH+ ERα+) with values ​​of 46.08 ± 1.36% vs. Controls (43.23 ± 1.13%). Furthermore, the percentage of somatotrophs expressing ERβ (GH+ ERβ+) also increased significantly after exposure to DEHP, with values ​​of 29.38 ± 0.65% in DEHP and 25.83 ± 0.81% in controls. DEHP induced a significant increase in the somatotroph population (60.8 ± 1.19%) compared to the control (47.33 ± 4.21%). In vitro, DEHP significantly increased the proliferation of somatotrophs (Ki67+ GH+), and the total somatotroph cell population, with values ​​of 16.08 ± 0.9% with DEHP 1nM, 20.81 ± 0.41% DEHP 10nM and 18.6 ± 0.39% DEHP 100nM, vs. control (11.98 ± 0.44%). These results showed that DEHP increased the expression of ERα and β in the somatotroph cell population of male rats in vivo and in vitro, increasing their proliferation, possibly as a consequence of the imbalance in the expression of ERs.
publisher Universidad Nacional Córdoba. Facultad de Ciencias Médicas. Secretaria de Ciencia y Tecnología
publishDate 2021
url https://revistas.unc.edu.ar/index.php/med/article/view/35024
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first_indexed 2024-09-03T21:02:58Z
last_indexed 2024-09-03T21:02:58Z
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spelling I10-R327-article-350242024-04-15T16:19:09Z Perinatal exposure to the phthalate "DEHP" alters the expression of estrogen receptors on somatotroph cells, increasing their proliferation La exposición perinatal al ftalato "DEHP" altera la expresión de los receptores estrogénicos de las células somatotropas, aumentando su proliferación Pérez , PA Toledo , J Picech , F Petiti , JP Mukdsi , JH Torres , AL De Paul , AL Gutierrez , S DI- (2-ETHYLHEXYL PHTHALATE) (DEHP) SOMATOTROPHS ALPHA AND BETA ESTROGEN RECEPTORS CELL PROLIFERATION DI-(2-ETHYLHEXYL FTALATO) (DEHP) SOMATOTROPAS RECEPTORES ESTROGENICOS ALFA Y BETA PROLIFERACION CELULAR Abstract:  Phthalates are chemicals widely used to make soft and flexible plastics. Di-2-ethylhexyl phthalate (DEHP) is the most commonly used, with high human exposure occurring from fetal development, acting as an endocrine disruptor by interfering with hormonal signaling. The objective of this study was to analyze the effect of perinatal exposure to DEHP on pituitary Estrogen receptor (ER)α and β expression and its impact on somatotroph cells.  For this, 10 pregnant Wistar rats were exposed by gavage to DEHP (200 µg / kg) or corn oil from day 0 of gestation, during pregnancy and lactation. For In vivo experiments, glands from 30 75-day-old male pups were used. For in vitro experiments, 3 pituitary cultures from 10 male rats were stimulated with 1, 10 or 100nM DEHP for 72 h. The percentages of somatotroph cells expressing ERα and β were quantified in pituitary cryosections by double immunostaining using confocal microscopy. Cell proliferation was quantified by double immunostaining (ki67+ GH+). The somatotroph cell population was quantified in vivo and in vitro by flow cytometry and immunocytochemistry, respectively. Statistical analysis: ANOVA Tukey (P <0.05). Our results showed that perinatal exposure to DEHP significantly increased the percentage of somatotrophs that expressed ERα (GH+ ERα+) with values ​​of 46.08 ± 1.36% vs. Controls (43.23 ± 1.13%). Furthermore, the percentage of somatotrophs expressing ERβ (GH+ ERβ+) also increased significantly after exposure to DEHP, with values ​​of 29.38 ± 0.65% in DEHP and 25.83 ± 0.81% in controls. DEHP induced a significant increase in the somatotroph population (60.8 ± 1.19%) compared to the control (47.33 ± 4.21%). In vitro, DEHP significantly increased the proliferation of somatotrophs (Ki67+ GH+), and the total somatotroph cell population, with values ​​of 16.08 ± 0.9% with DEHP 1nM, 20.81 ± 0.41% DEHP 10nM and 18.6 ± 0.39% DEHP 100nM, vs. control (11.98 ± 0.44%). These results showed that DEHP increased the expression of ERα and β in the somatotroph cell population of male rats in vivo and in vitro, increasing their proliferation, possibly as a consequence of the imbalance in the expression of ERs. Resumen:  Los ftalatos son productos químicos muy utilizados para fabricar plásticos blandos y flexibles. El Di-2-etilhexil ftalato (DEHP) es el más comúnmente utilizado, con alta exposición humana que ocurre desde el desarrollo fetal, actuando como disruptor endocrino al interferir con la señalización hormonal. El objetivo de este estudio fue analizar el efecto de la exposición perinatal a DEHP sobre expresión hipofisaria de receptores de estrógeno (RE) α y β y su impacto sobre las células somatotropas. Para ello, 10 ratas Wistar preñadas fueron expuestas por gavaje a DEHP (200 µg/kg) o aceite de maíz desde el día 0 de gestación, durante la preñez y lactancia. In vivo, se utilizaron glándulas de 30 crías macho de 75 días. Para experimentos in vitro,  3 cultivos hipofisarios de 10 ratas macho, fueron estimulados con 1, 10 o 100nM de DEHP durante 72 h. Los porcentajes de células somatotropas que expresan REα y β fueron cuantificadas en criosecciones hipofisarias mediante doble inmunomarcación utilizando microscopía confocal. Mediante doble inmunotinción (ki67+GH+) se cuantificó la proliferación celular. La población somatotropa fue cuantificada in vivo e in vitro por citometría de flujo e inmunocitoquímica, respectivamente. Análisis estadístico: ANOVA Tukey (P <0.05). Nuestros resultados mostraron que la exposición perinatal a DEHP aumentó significativamente el porcentaje de somatotropas que expresaron el REα (GH+ERα+) con valores de 46.08 ± 1.36% vs. Controles (43.23 ± 1.13%).  Ademas, el porcentaje de somatotropas que expresan REβ (GH+ERβ+) también incrementó significativamente luego de la exposición a DEHP, con valores de 29.38 ± 0,65% en DEHP y 25.83 ± 0.81% en controles. Además, DEHP indujo un aumento significativo de la población de somatotropas (60.8 ± 1.19%) frente al control (47.33 ± 4.21%). In vitro, DEHP aumentó significativamente la proliferación de somatotropas (Ki67+GH+), y la población de células somatotropas totales, con valores de 16.08 ± 0.9% con DEHP 1nM, 20.81 ± 0.41% DEHP 10nM y 18.6 ± 0.39% DEHP 100nM, vs. control (11.98 ± 0.44%). Estos resultados mostraron que DEHP aumentó la expresión de REα y β en la población de células somatotropas de ratas macho in vivo e in vitro, aumentando su proliferación, posiblemente como consecuencia del desequilibrio en la expresión de los RE. Universidad Nacional Córdoba. Facultad de Ciencias Médicas. Secretaria de Ciencia y Tecnología 2021-10-12 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion texto texto texto https://revistas.unc.edu.ar/index.php/med/article/view/35024 Revista de la Facultad de Ciencias Médicas de Córdoba.; Vol. 78 No. Suplemento (2021): Suplemento JIC XXII Revista de la Facultad de Ciencias Médicas de Córdoba; Vol. 78 Núm. Suplemento (2021): Suplemento JIC XXII Revista da Faculdade de Ciências Médicas de Córdoba; v. 78 n. Suplemento (2021): Suplemento JIC XXII 1853-0605 0014-6722 http://creativecommons.org/licenses/by-nc/4.0

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