Trophoblast turnover’s study in placentas of women with diabetes mellitus in vitro infected with T. cruzi
Abstract: The integrity of the placental trophoblast is maintained through the cytotrophoblast’s proliferation (CTB), fusion in the syncytiotrophoblast (STB), with controlled detachment of syncytial knots, a fundamental process for fetal development that is altered in diabetic placentas wi...
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| Formato: | Artículo revista |
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Universidad Nacional Córdoba. Facultad de Ciencias Médicas. Secretaria de Ciencia y Tecnología
2021
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| Acceso en línea: | https://revistas.unc.edu.ar/index.php/med/article/view/35015 |
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I10-R327-article-35015 |
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ojs |
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Universidad Nacional de Córdoba |
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I-10 |
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R-327 |
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Revista de la Facultad de Ciencias Médicas de Córdoba |
| format |
Artículo revista |
| topic |
Trypanosoma cruzi Diabetes human placenta trophoblast turnover Congenital Chagas Trypanosoma cruzi diabetes placenta humana renovación trofoblástica Chagas connatal |
| spellingShingle |
Trypanosoma cruzi Diabetes human placenta trophoblast turnover Congenital Chagas Trypanosoma cruzi diabetes placenta humana renovación trofoblástica Chagas connatal Salera , PB Moreira-Espinoza , MJ Piegari , M Díaz-Luján , CM Benizio, E Triquell , MF Fretes , RE Mezzano, L Trophoblast turnover’s study in placentas of women with diabetes mellitus in vitro infected with T. cruzi |
| topic_facet |
Trypanosoma cruzi Diabetes human placenta trophoblast turnover Congenital Chagas Trypanosoma cruzi diabetes placenta humana renovación trofoblástica Chagas connatal |
| author |
Salera , PB Moreira-Espinoza , MJ Piegari , M Díaz-Luján , CM Benizio, E Triquell , MF Fretes , RE Mezzano, L |
| author_facet |
Salera , PB Moreira-Espinoza , MJ Piegari , M Díaz-Luján , CM Benizio, E Triquell , MF Fretes , RE Mezzano, L |
| author_sort |
Salera , PB |
| title |
Trophoblast turnover’s study in placentas of women with diabetes mellitus in vitro infected with T. cruzi |
| title_short |
Trophoblast turnover’s study in placentas of women with diabetes mellitus in vitro infected with T. cruzi |
| title_full |
Trophoblast turnover’s study in placentas of women with diabetes mellitus in vitro infected with T. cruzi |
| title_fullStr |
Trophoblast turnover’s study in placentas of women with diabetes mellitus in vitro infected with T. cruzi |
| title_full_unstemmed |
Trophoblast turnover’s study in placentas of women with diabetes mellitus in vitro infected with T. cruzi |
| title_sort |
trophoblast turnover’s study in placentas of women with diabetes mellitus in vitro infected with t. cruzi |
| description |
Abstract:
The integrity of the placental trophoblast is maintained through the cytotrophoblast’s proliferation (CTB), fusion in the syncytiotrophoblast (STB), with controlled detachment of syncytial knots, a fundamental process for fetal development that is altered in diabetic placentas with greater susceptibility to infections. Trypanosoma cruzi (T. cruzi) can cause the congenital transmission of Chagas disease, altering the turnover of the human trophoblast in in vitro studies. Little is known about the trophoblast renewal process in diabetic and parasite-infected placentas. The aim of this work was to analyze in vitro the human trophoblast turnover process in placentas from diabetic women infected with T. cruzi. Human placentas at term from cesarean sections of clinically healthy women (control) and with diabetes (n = 6) were used, respecting ethical and legal aspects. The explants were co-cultured with 1x106 trypomastigotes of T. cruzi, Tulahuen strain, for 24 hours. In sections stained with H/E were quantified: number of syncytial knots, perivillous fibrin and STB detachment’s areas (%). Immunohistochemistry was performed for Ki67 (proliferation of CTB) and Cytokeratin-7 (trophoblastic integrity). Fiji ImageJ was used for quantification. Statistics: ANOVA- Bonferroni test (p<0.05). A significant increase in the number of syncytial knots (91%; p = 0.0017), in intervillous fibrin deposits (97%; p = 0.0002) and in STB detachment (86.6%; p = 0.0075) was observed in infected diabetic placentas, compared to controls. The proliferation index (Ki67) increased significantly (52%) in infected diabetic placentas, compared to controls (p = 0.0047). While the expression of CTK7 decreased by 47.5% in diabetic placentas without infection, with respect to the control (p<0.0001), this difference being less in diabetics co-cultured with T. cruzi (15%). These results suggest that the dynamics of trophoblastic turnover is modified in diabetic and T. cruzi-infected placentas, altering the defense mechanisms of the placental barrier, which could further increase the probability of the parasite’s congenital transmission |
| publisher |
Universidad Nacional Córdoba. Facultad de Ciencias Médicas. Secretaria de Ciencia y Tecnología |
| publishDate |
2021 |
| url |
https://revistas.unc.edu.ar/index.php/med/article/view/35015 |
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2024-09-03T21:02:57Z |
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I10-R327-article-350152024-04-15T16:19:09Z Trophoblast turnover’s study in placentas of women with diabetes mellitus in vitro infected with T. cruzi Estudio de la renovación del trofoblasto en placentas de mujeres con diabetes mellitus infectadas in vitro con T. cruzi Salera , PB Moreira-Espinoza , MJ Piegari , M Díaz-Luján , CM Benizio, E Triquell , MF Fretes , RE Mezzano, L Trypanosoma cruzi Diabetes human placenta trophoblast turnover Congenital Chagas Trypanosoma cruzi diabetes placenta humana renovación trofoblástica Chagas connatal Abstract: The integrity of the placental trophoblast is maintained through the cytotrophoblast’s proliferation (CTB), fusion in the syncytiotrophoblast (STB), with controlled detachment of syncytial knots, a fundamental process for fetal development that is altered in diabetic placentas with greater susceptibility to infections. Trypanosoma cruzi (T. cruzi) can cause the congenital transmission of Chagas disease, altering the turnover of the human trophoblast in in vitro studies. Little is known about the trophoblast renewal process in diabetic and parasite-infected placentas. The aim of this work was to analyze in vitro the human trophoblast turnover process in placentas from diabetic women infected with T. cruzi. Human placentas at term from cesarean sections of clinically healthy women (control) and with diabetes (n = 6) were used, respecting ethical and legal aspects. The explants were co-cultured with 1x106 trypomastigotes of T. cruzi, Tulahuen strain, for 24 hours. In sections stained with H/E were quantified: number of syncytial knots, perivillous fibrin and STB detachment’s areas (%). Immunohistochemistry was performed for Ki67 (proliferation of CTB) and Cytokeratin-7 (trophoblastic integrity). Fiji ImageJ was used for quantification. Statistics: ANOVA- Bonferroni test (p<0.05). A significant increase in the number of syncytial knots (91%; p = 0.0017), in intervillous fibrin deposits (97%; p = 0.0002) and in STB detachment (86.6%; p = 0.0075) was observed in infected diabetic placentas, compared to controls. The proliferation index (Ki67) increased significantly (52%) in infected diabetic placentas, compared to controls (p = 0.0047). While the expression of CTK7 decreased by 47.5% in diabetic placentas without infection, with respect to the control (p<0.0001), this difference being less in diabetics co-cultured with T. cruzi (15%). These results suggest that the dynamics of trophoblastic turnover is modified in diabetic and T. cruzi-infected placentas, altering the defense mechanisms of the placental barrier, which could further increase the probability of the parasite’s congenital transmission Resumen: La integridad del trofoblasto placentario es mantenida a través de la proliferación del citotrofoblasto (CTB), fusión en el sinciciotrofoblasto (STB) con desprendimiento controlado de nudos sinciciales, proceso fundamental para el desarrollo fetal y que se altera en placentas diabéticas, con mayor susceptibilidad a infecciones. El Trypanosoma cruzi (T. cruzi) puede producir la transmisión congénita de Chagas, alterando la renovación del trofoblasto humano en estudios in vitro. Poco se conoce sobre el proceso de renovación del trofoblasto en placentas diabéticas e infectadas con el parásito. El objetivo de este trabajo fue analizar in vitro el proceso de renovación del trofoblasto humano en placentas de mujeres diabéticas, infectadas con T. cruzi. Se utilizaron placentas humanas a término provenientes de cesáreas de mujeres clínicamente sanas (control) y con diabetes (n=6), respetando aspectos Ético-legales. Los explantos fueron co-cultivados con 1x106 tripomastigotes de T. cruzi, cepa Tulahuen, durante 24hs. En cortes teñidos con H/E se cuantificó: cantidad de nudos sinciciales, área de fibrina perivellosa y desprendimiento de STB (%). Se realizó inmunohistoquímica para Ki67 (proliferación del CTB) y Citokeratina-7 (integridad trofoblástica). Cuantificación con Fiji ImageJ. Estadística: ANOVA- test de Bonferroni (p<0,05). Se observó un aumento significativo en la cantidad de nudos sinciciales (91%; p=0,0017), en los depósitos de fibrina intervellosa (97%; p= 0,0002) y en el desprendimiento de STB (86.6%; p=0,0075) en placentas diabéticas infectadas, con respecto a los controles. El índice de proliferación (Ki67) aumentó significativamente (52%) en las placentas diabéticas infectadas, con respecto a los controles (p=0,0047). Mientras que la expresión de CTK7 disminuyó un 47.5% en placentas diabéticas sin infección, con respecto al control (p<0.0001), siendo menor esta diferencia en diabéticas co-cultivadas con T. cruzi (15%). Estos resultados sugieren que la dinámica de la renovación trofoblástica se modifica en placentas diabéticas e infectadas con T. cruzi, alterando los mecanismos de defensa de la barrera placentaria, lo que podría aumentar aún más la probabilidad de transmisión congénita del parásito. Universidad Nacional Córdoba. Facultad de Ciencias Médicas. Secretaria de Ciencia y Tecnología 2021-10-12 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion texto texto texto https://revistas.unc.edu.ar/index.php/med/article/view/35015 Revista de la Facultad de Ciencias Médicas de Córdoba.; Vol. 78 No. Suplemento (2021): Suplemento JIC XXII Revista de la Facultad de Ciencias Médicas de Córdoba; Vol. 78 Núm. Suplemento (2021): Suplemento JIC XXII Revista da Faculdade de Ciências Médicas de Córdoba; v. 78 n. Suplemento (2021): Suplemento JIC XXII 1853-0605 0014-6722 http://creativecommons.org/licenses/by-nc/4.0 |