Vector competence of Aedes aegypti populations from central Argentina for Mayaro virus (Alphavirus)
Abstract: In recent decades, diseases caused by arboviruses have increased their global distribution and incidence on the human population. The Mayaro virus (MAYV) endemic of South and Central America, is causal agent of polyarthritis diseases. In urban regions MAYV could be transmitt...
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| Formato: | Artículo revista |
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Universidad Nacional Córdoba. Facultad de Ciencias Médicas. Secretaria de Ciencia y Tecnología
2021
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| Acceso en línea: | https://revistas.unc.edu.ar/index.php/med/article/view/34954 |
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I10-R327-article-34954 |
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ojs |
| institution |
Universidad Nacional de Córdoba |
| institution_str |
I-10 |
| repository_str |
R-327 |
| container_title_str |
Revista de la Facultad de Ciencias Médicas de Córdoba |
| format |
Artículo revista |
| topic |
aedes arboviruses alphavirus infections Argentina aedes culicidae arboviruses alphavirus infections argentina . |
| spellingShingle |
aedes arboviruses alphavirus infections Argentina aedes culicidae arboviruses alphavirus infections argentina . Beranek , M Giayetto , O Rucci , K Peralta , G Fischer , S Diaz , A Vector competence of Aedes aegypti populations from central Argentina for Mayaro virus (Alphavirus) |
| topic_facet |
aedes arboviruses alphavirus infections Argentina aedes culicidae arboviruses alphavirus infections argentina . |
| author |
Beranek , M Giayetto , O Rucci , K Peralta , G Fischer , S Diaz , A |
| author_facet |
Beranek , M Giayetto , O Rucci , K Peralta , G Fischer , S Diaz , A |
| author_sort |
Beranek , M |
| title |
Vector competence of Aedes aegypti populations from central Argentina for Mayaro virus (Alphavirus) |
| title_short |
Vector competence of Aedes aegypti populations from central Argentina for Mayaro virus (Alphavirus) |
| title_full |
Vector competence of Aedes aegypti populations from central Argentina for Mayaro virus (Alphavirus) |
| title_fullStr |
Vector competence of Aedes aegypti populations from central Argentina for Mayaro virus (Alphavirus) |
| title_full_unstemmed |
Vector competence of Aedes aegypti populations from central Argentina for Mayaro virus (Alphavirus) |
| title_sort |
vector competence of aedes aegypti populations from central argentina for mayaro virus (alphavirus) |
| description |
Abstract:
In recent decades, diseases caused by arboviruses have increased their global distribution and incidence on the human population. The Mayaro virus (MAYV) endemic of South and Central America, is causal agent of polyarthritis diseases. In urban regions MAYV could be transmitted by Aedes aegypti. Vector competence is the ability of a mosquito population to become infected by feeding on a viremic host and transmitting it to a susceptible host. The aim of this study was to evaluate the susceptibility to infection and transmission by MAYV in populations of Ae. aegypti from Córdoba and Buenos Aires cities. Eggs were collected in the before mentioned cities and colonies were established in the Arbovirus Laboratory facilities (InViV). Five to 7 days old mosquito females were exposed orally with five viral load ranging 1.9 to 5.8 log10 PFU/ml. Fully engorged females were apart and kept at 27oC, 70% humidity and 12:12 photoperiod during 9 days. Abdomens, legs and salivary secretion were extracted for presence of infective viral particles by means of plaquing assay on Vero cell monolayers. The infection rate (IR = number of mosquitoes with presence of MAYV in abdomen/number of mosquitoes analyzed), dissemination rate (DR = number of mosquitoes with presence of MAYV on legs/number of mosquitoes analyzed) and transmission rate (TR = number of mosquitoes with presence of MAYV in saliva/number of mosquitoes analyzed) were estimated for each population. Córdoba population showed IR between 3.1% and 22.2% with viral load of 4.7 to 5.2 log10 PFU/ml, respectively. In Buenos Aires population the IR were 5.3% to 41.2% with viral loads of 3.8 to 5.8 log10 PFU/ml, respectively. TR was 4% (Córdoba) and 5.8% (Buenos Aires) only in individuals exposed with the highest viral load. Our results show that Ae. aegypti populations from the main urban cities of Argentina do not have a high susceptibility to infection by MAYV and only transmit the virus in a small proportion at high viral loads.
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| publisher |
Universidad Nacional Córdoba. Facultad de Ciencias Médicas. Secretaria de Ciencia y Tecnología |
| publishDate |
2021 |
| url |
https://revistas.unc.edu.ar/index.php/med/article/view/34954 |
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2024-09-03T21:02:52Z |
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2024-09-03T21:02:52Z |
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I10-R327-article-349542024-04-15T16:19:09Z Vector competence of Aedes aegypti populations from central Argentina for Mayaro virus (Alphavirus) Competencia vectorial de Aedes aegypti para el virus Mayaro (Alphavirus) en poblaciones del centro de Argentina A Beranek , M Giayetto , O Rucci , K Peralta , G Fischer , S Diaz , A aedes arboviruses alphavirus infections Argentina aedes culicidae arboviruses alphavirus infections argentina . Abstract: In recent decades, diseases caused by arboviruses have increased their global distribution and incidence on the human population. The Mayaro virus (MAYV) endemic of South and Central America, is causal agent of polyarthritis diseases. In urban regions MAYV could be transmitted by Aedes aegypti. Vector competence is the ability of a mosquito population to become infected by feeding on a viremic host and transmitting it to a susceptible host. The aim of this study was to evaluate the susceptibility to infection and transmission by MAYV in populations of Ae. aegypti from Córdoba and Buenos Aires cities. Eggs were collected in the before mentioned cities and colonies were established in the Arbovirus Laboratory facilities (InViV). Five to 7 days old mosquito females were exposed orally with five viral load ranging 1.9 to 5.8 log10 PFU/ml. Fully engorged females were apart and kept at 27oC, 70% humidity and 12:12 photoperiod during 9 days. Abdomens, legs and salivary secretion were extracted for presence of infective viral particles by means of plaquing assay on Vero cell monolayers. The infection rate (IR = number of mosquitoes with presence of MAYV in abdomen/number of mosquitoes analyzed), dissemination rate (DR = number of mosquitoes with presence of MAYV on legs/number of mosquitoes analyzed) and transmission rate (TR = number of mosquitoes with presence of MAYV in saliva/number of mosquitoes analyzed) were estimated for each population. Córdoba population showed IR between 3.1% and 22.2% with viral load of 4.7 to 5.2 log10 PFU/ml, respectively. In Buenos Aires population the IR were 5.3% to 41.2% with viral loads of 3.8 to 5.8 log10 PFU/ml, respectively. TR was 4% (Córdoba) and 5.8% (Buenos Aires) only in individuals exposed with the highest viral load. Our results show that Ae. aegypti populations from the main urban cities of Argentina do not have a high susceptibility to infection by MAYV and only transmit the virus in a small proportion at high viral loads. Resumen: En las últimas décadas, las enfermedades ocasionadas por arbovirus han aumentado su distribución global e incidencia sobre la población humana. El virus Mayaro (VMAY) endémico de América del Sur y Central, es el agente causal de poliartritis al igual que el virus Chikungunya. En regiones urbanas podría ser transmitido por Aedes aegypti. La competencia vectorial es la habilidad de una población de mosquitos de infectarse al alimentarse de una comida virémica y transmitirlo a un hospedador susceptible. El objetivo de este trabajo fue evaluar la susceptibilidad a la infección y transmisión por el VMAY en poblaciones de Ae. aegypti procedentes de las ciudades de Córdoba y Buenos Aires. Se colectaron huevos en las localidades citadas para el establecimiento de las colonias en el Laboratorio de Arbovrius (InViV). Las hembras se infectaron por vía oral con cinco cargas virales comprendidas entre 1,9 a 5,8 log10 UFP/ml. Las hembras completamente alimentadas fueron separadas y mantenidas a 27oC, 70% humedad y fotoperíodo 12:12 durante 9 días. Se les extrajo abdomen, patas y secreción salival. La presencia de partículas virales infectivas se detectó mediante plaqueo en monocapas de células Vero. Se determinó la tasa de infección (TI = número de mosquitos con presencia de VMAY en abdomen/número de mosquitos analizados), tasa de diseminación (TD = número de mosquitos con presencia de VMAY en patas/número de mosquitos analizados) y la tasa de transmisión (TT = número de mosquitos con presencia de VMAY en salivas/número de mosquitos analizados). La población de Córdoba presentó TI entre 3,1% y 22,2% con cargas virales 4,7 a 5,2 log10 UFP/ml, respectivamente. Las TI para la población de Buenos Aires fueron entre 5,3% a 41,2% con cargas virales de 3,8 a 5,8 log10 UFP/ml. Sólo se observó virus en saliva en individuos expuestos a la mayor carga viral en ambas poblaciones, siendo la TT 4% (Córdoba) y 5,8% (Buenos Aires). Nuestros resultados indican que las poblaciones de Ae. aegypti de los principales centros urbanos de Argentina no poseen una elevada susceptibilidad a la infección por VMAY y sólo son capaces de transmitirlo en baja proporción a elevadas cargas virales. . Universidad Nacional Córdoba. Facultad de Ciencias Médicas. Secretaria de Ciencia y Tecnología 2021-10-12 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion texto texto texto https://revistas.unc.edu.ar/index.php/med/article/view/34954 Revista de la Facultad de Ciencias Médicas de Córdoba.; Vol. 78 No. Suplemento (2021): Suplemento JIC XXII Revista de la Facultad de Ciencias Médicas de Córdoba; Vol. 78 Núm. Suplemento (2021): Suplemento JIC XXII Revista da Faculdade de Ciências Médicas de Córdoba; v. 78 n. Suplemento (2021): Suplemento JIC XXII 1853-0605 0014-6722 http://creativecommons.org/licenses/by-nc/4.0 |