Articular cartilage in osteoarthritic patients: efectos of diclofenac, celecoxib and glucosamine sulfate on inflammatory markers
Osteoarthritis is a chronic and progressive joint disease. It is established by a complex process involving mechanical and biological alterations of the musculoskeletal system, which are generated by a great variety of interactions between enetic factors and extrinsic i...
| Autores principales: | , , , |
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| Formato: | Artículo revista |
| Lenguaje: | Español |
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Universidad Nacional Córdoba. Facultad de Ciencias Médicas. Secretaria de Ciencia y Tecnología
2019
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| Materias: | |
| Acceso en línea: | https://revistas.unc.edu.ar/index.php/med/article/view/25897 |
| Aporte de: |
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I10-R327-article-25897 |
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ojs |
| institution |
Universidad Nacional de Córdoba |
| institution_str |
I-10 |
| repository_str |
R-327 |
| container_title_str |
Revista de la Facultad de Ciencias Médicas de Córdoba |
| language |
Español |
| format |
Artículo revista |
| topic |
Osteoarthritis chondrocytes colagenasa óxido nítrico antiinflamatorios osteoteoartritismetaloproteasas- oxido nítrico anti-inflamatoriosglucosamina |
| spellingShingle |
Osteoarthritis chondrocytes colagenasa óxido nítrico antiinflamatorios osteoteoartritismetaloproteasas- oxido nítrico anti-inflamatoriosglucosamina Brizuela, Nilda Y Montrull, Hilda L Demurtas , Silvia L Meirovich, Carlos I Articular cartilage in osteoarthritic patients: efectos of diclofenac, celecoxib and glucosamine sulfate on inflammatory markers |
| topic_facet |
Osteoarthritis chondrocytes colagenasa óxido nítrico antiinflamatorios osteoteoartritismetaloproteasas- oxido nítrico anti-inflamatoriosglucosamina |
| author |
Brizuela, Nilda Y Montrull, Hilda L Demurtas , Silvia L Meirovich, Carlos I |
| author_facet |
Brizuela, Nilda Y Montrull, Hilda L Demurtas , Silvia L Meirovich, Carlos I |
| author_sort |
Brizuela, Nilda Y |
| title |
Articular cartilage in osteoarthritic patients: efectos of diclofenac, celecoxib and glucosamine sulfate on inflammatory markers |
| title_short |
Articular cartilage in osteoarthritic patients: efectos of diclofenac, celecoxib and glucosamine sulfate on inflammatory markers |
| title_full |
Articular cartilage in osteoarthritic patients: efectos of diclofenac, celecoxib and glucosamine sulfate on inflammatory markers |
| title_fullStr |
Articular cartilage in osteoarthritic patients: efectos of diclofenac, celecoxib and glucosamine sulfate on inflammatory markers |
| title_full_unstemmed |
Articular cartilage in osteoarthritic patients: efectos of diclofenac, celecoxib and glucosamine sulfate on inflammatory markers |
| title_sort |
articular cartilage in osteoarthritic patients: efectos of diclofenac, celecoxib and glucosamine sulfate on inflammatory markers |
| description |
Osteoarthritis is a chronic and progressive joint disease. It is established by a complex process involving mechanical and biological alterations of the musculoskeletal system, which are generated by a great variety of interactions between enetic factors and extrinsic injuries. The pathogenesis of this disease is related to an increased and clivergent production of infiammatory markers and proteolytic enzymes that promote the degradation and destruction of the extracellular matrix of articular and periarticular tissues. Cartilage samples were taken frorn 20 osteoarthritic patients during programmed surgical interventions. The cartilage samples were cultu red in Dulbecco-Eagle medium. With or without the addition of SAIDs or modulators of chondrocyte metabolism. The content of nitric oxide in the supernatant was quantified using the Griess reaction: the concentration of MMP- 1 was quantificd vía double-sandwich ELISA. Untreated chondrocyte cultures produced 1950 ± 665ng/ml MMP-1. Wíth the addition of Diclofenac this value decreased to 1140 ± 155 ng/mi, although his difference was not statistically significant (p<0 ,06). However, in the presence of Celecoxib the level significantly dropped to 760 ± 75 ng/mI (p<0,01). Although the addition of glucosarnine did not produce such a noticeable reduction in The level of MMP- 1 (950±89 ng/mi), it was statistically significant (p<0,05). On the contraly, none of the drugs (Diclofenac, Celecoxib, Glucosamine) modified the level of nitric oxide which had a mean value of 47,3 ± 4.911M in the Control samples. This investigation evidenced the inability of Diclofenac to significantly modifr the production of proteolytic enzymes in osteoarthritic chondrocyte cultures. However, both Celecoxib and Glucosamine significantly reduced the production of MMP- 1. On the contrary, none of the drugs used in this study managed to modify the concentration of nitric oxide. To the present day, no drugs have been found to be efficient in altering the natural course of the disease, requiring further investigation |
| publisher |
Universidad Nacional Córdoba. Facultad de Ciencias Médicas. Secretaria de Ciencia y Tecnología |
| publishDate |
2019 |
| url |
https://revistas.unc.edu.ar/index.php/med/article/view/25897 |
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2024-09-03T21:01:17Z |
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2024-09-03T21:01:17Z |
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I10-R327-article-258972024-08-27T18:26:36Z Articular cartilage in osteoarthritic patients: efectos of diclofenac, celecoxib and glucosamine sulfate on inflammatory markers Efectos de diclofenac, celecoxib y sulfato de glucosamina sobre marcadores inflamatorios en cultivo de condrocitos de pacientes con osteoartritis Brizuela, Nilda Y Montrull, Hilda L Demurtas , Silvia L Meirovich, Carlos I Osteoarthritis chondrocytes colagenasa óxido nítrico antiinflamatorios osteoteoartritismetaloproteasas- oxido nítrico anti-inflamatoriosglucosamina Osteoarthritis is a chronic and progressive joint disease. It is established by a complex process involving mechanical and biological alterations of the musculoskeletal system, which are generated by a great variety of interactions between enetic factors and extrinsic injuries. The pathogenesis of this disease is related to an increased and clivergent production of infiammatory markers and proteolytic enzymes that promote the degradation and destruction of the extracellular matrix of articular and periarticular tissues. Cartilage samples were taken frorn 20 osteoarthritic patients during programmed surgical interventions. The cartilage samples were cultu red in Dulbecco-Eagle medium. With or without the addition of SAIDs or modulators of chondrocyte metabolism. The content of nitric oxide in the supernatant was quantified using the Griess reaction: the concentration of MMP- 1 was quantificd vía double-sandwich ELISA. Untreated chondrocyte cultures produced 1950 ± 665ng/ml MMP-1. Wíth the addition of Diclofenac this value decreased to 1140 ± 155 ng/mi, although his difference was not statistically significant (p<0 ,06). However, in the presence of Celecoxib the level significantly dropped to 760 ± 75 ng/mI (p<0,01). Although the addition of glucosarnine did not produce such a noticeable reduction in The level of MMP- 1 (950±89 ng/mi), it was statistically significant (p<0,05). On the contraly, none of the drugs (Diclofenac, Celecoxib, Glucosamine) modified the level of nitric oxide which had a mean value of 47,3 ± 4.911M in the Control samples. This investigation evidenced the inability of Diclofenac to significantly modifr the production of proteolytic enzymes in osteoarthritic chondrocyte cultures. However, both Celecoxib and Glucosamine significantly reduced the production of MMP- 1. On the contrary, none of the drugs used in this study managed to modify the concentration of nitric oxide. To the present day, no drugs have been found to be efficient in altering the natural course of the disease, requiring further investigation La osteoartritis (OA) es una enfermedad articular crónica, progresiva que se instala como consecuencia de un proceso complejo que involucra alteraciones mecánicas y biológicas del sistema músculo-esquelético, siendo resultante de múltiples interacciones entre factores genéticos e injurias extrínsecas. La patogenia de esta enfermedad se relaciona con alta y desviada producción de citokinas flogógenas y de enzimas proteolíticas. Que degradan y destruyen la matriz extracelular en tejidos articulares y peri-articulares. Se estudiaron 20 casos con OA, de los cuales se obtuvo cartílago durante intervenciones quirúrgicas programadas. El cartílago se cultivó en medio Dulbecco- Eagle, con o sin agregado de AINEs o condromoduladores. En los sobrenadantes se determinaron óxido nítrico por reacción de Griess y medición espectrofotométrica; y colagenasa por ELISA doble sándwich en presencia de anticuerpos monoclonales. En ausencia de AINEs, los cultivos de condrocitos produjeron 1950 ± 665ng/m1 de MMP- 1. La adición de Diclofenac redujo esa cifra a 1140± 155 ng/mI, aunque esta diferencia no fue estadisticamente significativa, (p<0,06). Por el contrario, Celecoxib redujo el nivel de la enzima a 760 ± 75 ng/ml (p<0,01) y la Glucosamina también provocó un descenso (950 ± 89 ng/mI) significativo (p<0.05). Los niveles de ON en ausencia de AINEs llegaron a 47,3 ± 4,9 pM. Su producción no varió significativamente con la adición de Diclofrnac, Celecoxib o Glucosamina (p=ns). Los resultados indicarían la incapacidad de Diclofenac para modificar la generación de enzimas proteolíticas, mientras que Celecoxib y Gucosamina disminuyen su producciónsignificativamente. Ninguno de los fármacos utilizados en nuestro trabajo ha logrado alterar la concentración de ON. Muchos interrogantes quedan aún sin resolver y todavía se carece de fármacos de eficacia comprobada para alterar el curso natural de la enfermedad. Universidad Nacional Córdoba. Facultad de Ciencias Médicas. Secretaria de Ciencia y Tecnología 2019-10-22 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf https://revistas.unc.edu.ar/index.php/med/article/view/25897 Revista de la Facultad de Ciencias Médicas de Córdoba.; Vol. 64 No. 2 (2007); 9 - 15 Revista de la Facultad de Ciencias Médicas de Córdoba; Vol. 64 Núm. 2 (2007); 9 - 15 Revista da Faculdade de Ciências Médicas de Córdoba; v. 64 n. 2 (2007); 9 - 15 1853-0605 0014-6722 spa https://revistas.unc.edu.ar/index.php/med/article/view/25897/27715 Derechos de autor 2019 Universidad Nacional de Córdoba https://creativecommons.org/licenses/by-nc/4.0 |