In vitro Modulating Activity of aqueous extracts from American Plants on Chlorpyrifos-induced toxicity on Murine Splenocytes

Background: Chlorpyrifos is an highly toxic pesticide, which can induce immunotoxicity with deleterious effects on health worldwide. On the other hand, American plants can provide derivatives with protective and immunostimulating activity. Thus, plant potential against chlorpyrifos should be assayed...

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Detalles Bibliográficos
Autores principales: Scotta, Ana Verónica, Bongiovanni, Guillermina Azucena, Soria, Elio Andrés
Formato: Artículo revista
Lenguaje:Español
Publicado: Universidad Nacional Córdoba. Facultad de Ciencias Médicas. Secretaria de Ciencia y Tecnología 2017
Materias:
A
phytotherapy
Ilex paraguariensis
lymphocytes
oxidation-reduction
quebracho
tea
Concanavalina A
Fitoterapia
Linfocitos
Oxidación-Reducción
Quebracho
Té
Acceso en línea:https://revistas.unc.edu.ar/index.php/med/article/view/15361
Aporte de:
Revista de la Facultad de Ciencias Médicas de Córdoba de Universidad Nacional de Córdoba
id I10-R327-article-15361
record_format ojs
institution Universidad Nacional de Córdoba
institution_str I-10
repository_str R-327
container_title_str Revista de la Facultad de Ciencias Médicas de Córdoba
language Español
format Artículo revista
topic A
phytotherapy
Ilex paraguariensis
lymphocytes
oxidation-reduction
quebracho
tea
Concanavalina A
Fitoterapia
Ilex paraguariensis
Linfocitos
Oxidación-Reducción
Quebracho
Té
spellingShingle A
phytotherapy
Ilex paraguariensis
lymphocytes
oxidation-reduction
quebracho
tea
Concanavalina A
Fitoterapia
Ilex paraguariensis
Linfocitos
Oxidación-Reducción
Quebracho
Té
Scotta, Ana Verónica
Bongiovanni, Guillermina Azucena
Soria, Elio Andrés
In vitro Modulating Activity of aqueous extracts from American Plants on Chlorpyrifos-induced toxicity on Murine Splenocytes
topic_facet A
phytotherapy
Ilex paraguariensis
lymphocytes
oxidation-reduction
quebracho
tea
Concanavalina A
Fitoterapia
Ilex paraguariensis
Linfocitos
Oxidación-Reducción
Quebracho
Té
author Scotta, Ana Verónica
Bongiovanni, Guillermina Azucena
Soria, Elio Andrés
author_facet Scotta, Ana Verónica
Bongiovanni, Guillermina Azucena
Soria, Elio Andrés
author_sort Scotta, Ana Verónica
title In vitro Modulating Activity of aqueous extracts from American Plants on Chlorpyrifos-induced toxicity on Murine Splenocytes
title_short In vitro Modulating Activity of aqueous extracts from American Plants on Chlorpyrifos-induced toxicity on Murine Splenocytes
title_full In vitro Modulating Activity of aqueous extracts from American Plants on Chlorpyrifos-induced toxicity on Murine Splenocytes
title_fullStr In vitro Modulating Activity of aqueous extracts from American Plants on Chlorpyrifos-induced toxicity on Murine Splenocytes
title_full_unstemmed In vitro Modulating Activity of aqueous extracts from American Plants on Chlorpyrifos-induced toxicity on Murine Splenocytes
title_sort in vitro modulating activity of aqueous extracts from american plants on chlorpyrifos-induced toxicity on murine splenocytes
description Background: Chlorpyrifos is an highly toxic pesticide, which can induce immunotoxicity with deleterious effects on health worldwide. On the other hand, American plants can provide derivatives with protective and immunostimulating activity. Thus, plant potential against chlorpyrifos should be assayed. Objective: To identify bioactive aqueous extracts from Lantana grisebachii (LG), Aspidosperma quebracho-blanco (AQ), Peumus boldus (PB), and Ilex paraguariensis (IP), against chlorpyrifos-induced toxicity on female Balb/c splenocytes. Material and method: Splenocytes were treated in vitro for 72 hours with 0-35 µg/mL of chlorpyrifos, 0-100 µg/mL of each extract (LG, AQ, PB, IP), and 0-5 µg/mL of concanavalin A. Then, cellular viability and death (resazurin-based and propidium iodide stainings), hydroperoxides, lipoperoxides (xylenol orange-based assay), ?-glutamyl transpeptidase activity (Szasz method) were measured and analyzed statistically. Results: Chlorpyrifos reduced splenocyte viability in a dose-dependent manner, which was counteracted by AQ and IP, which was less active in concanavalin A-responsive cells (p<0.05). Chlorpyrifos toxicity involved ?-glutamyltranspeptidase induction with a consequent peroxide reduction, whereas AQ and mainly IP antagonized these responses (p<0.05). Conclusions: The extracts of Ilex paraguariensis and Aspidosperma quebracho-blanco protected splenocytes in vitro against chlorpyrifos. This effect depended on cellular type, given that concanavalin A-responsive cells were more susceptible to this toxic.
publisher Universidad Nacional Córdoba. Facultad de Ciencias Médicas. Secretaria de Ciencia y Tecnología
publishDate 2017
url https://revistas.unc.edu.ar/index.php/med/article/view/15361
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AT bongiovanniguillerminaazucena invitromodulatingactivityofaqueousextractsfromamericanplantsonchlorpyrifosinducedtoxicityonmurinesplenocytes
AT soriaelioandres invitromodulatingactivityofaqueousextractsfromamericanplantsonchlorpyrifosinducedtoxicityonmurinesplenocytes
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spelling I10-R327-article-153612024-08-27T18:20:20Z In vitro Modulating Activity of aqueous extracts from American Plants on Chlorpyrifos-induced toxicity on Murine Splenocytes Actividad moduladora in vitro de extractos acuosos de plantas americanas sobre la toxicidad inducida por clorpirifos en esplenocitos murinos Scotta, Ana Verónica Bongiovanni, Guillermina Azucena Soria, Elio Andrés A phytotherapy Ilex paraguariensis lymphocytes oxidation-reduction quebracho tea Concanavalina A Fitoterapia Ilex paraguariensis Linfocitos Oxidación-Reducción Quebracho Té Background: Chlorpyrifos is an highly toxic pesticide, which can induce immunotoxicity with deleterious effects on health worldwide. On the other hand, American plants can provide derivatives with protective and immunostimulating activity. Thus, plant potential against chlorpyrifos should be assayed. Objective: To identify bioactive aqueous extracts from Lantana grisebachii (LG), Aspidosperma quebracho-blanco (AQ), Peumus boldus (PB), and Ilex paraguariensis (IP), against chlorpyrifos-induced toxicity on female Balb/c splenocytes. Material and method: Splenocytes were treated in vitro for 72 hours with 0-35 µg/mL of chlorpyrifos, 0-100 µg/mL of each extract (LG, AQ, PB, IP), and 0-5 µg/mL of concanavalin A. Then, cellular viability and death (resazurin-based and propidium iodide stainings), hydroperoxides, lipoperoxides (xylenol orange-based assay), ?-glutamyl transpeptidase activity (Szasz method) were measured and analyzed statistically. Results: Chlorpyrifos reduced splenocyte viability in a dose-dependent manner, which was counteracted by AQ and IP, which was less active in concanavalin A-responsive cells (p<0.05). Chlorpyrifos toxicity involved ?-glutamyltranspeptidase induction with a consequent peroxide reduction, whereas AQ and mainly IP antagonized these responses (p<0.05). Conclusions: The extracts of Ilex paraguariensis and Aspidosperma quebracho-blanco protected splenocytes in vitro against chlorpyrifos. This effect depended on cellular type, given that concanavalin A-responsive cells were more susceptible to this toxic. Antecedentes: Clorpirifos es un pesticida altamente tóxico, que puede producir inmunotoxicidad con efectos deletéreos sobre la salud a nivel mundial. Por otro lado, las plantas americanas pueden tener derivados con actividad protectora e inmunoestimulante. Por lo tanto, debe evaluarse el potencial de estas plantas frente a clorpirifos. Objetivo: Identificar extractos acuosos bioactivos de Lantana grisebachii (LG), Aspidosperma quebracho-blanco (AQ), Peumus boldus (PB), e Ilex paraguariensis (IP), contra la toxicidad de clorpirifos sobre esplenocitos de hembras Balb/c. Materiales y Métodos: Esplenocitos fueron tratados in vitro por 72 horas con 0-35 µg/mL de clorpirifos, 0-100 µg/mL de cada extracto (LG, AQ, PB, IP) y 0-5 µg/mL de concanavalina A. Luego, se midió y analizó estadísticamente viabilidad y muerte celular (tinciones de resazurina y yoduro de propidio), hidroperóxidos, lipoperóxidos (ensayos basados en naranja de xilenol), actividad de la ?-glutamiltranspeptidasa (método de Szasz). Resultados: Clorpirifos redujo la viabilidad celular de forma dosis dependiente, lo que fue contrarrestado por AQ e IP, los que fueron menos activos en las células inducibles por concanavalina A (p<0,05). La toxicidad por clorpirifos implicó la inducción de la ?-glutamiltranspeptidasa con la consecuente reducción de los peróxidos, mientras que AQ y principalmente IP antagonizaron dichas respuestas (p<0,05). Conclusiones: Los extractos de Ilex paraguariensis y Aspidosperma quebracho-blanco protegieron in vitro a los esplenocitos frente a clorpirifos. Este efecto dependió del tipo celular, dado que las células inducibles por concanavalina A fueron más susceptibles a este tóxico. Universidad Nacional Córdoba. Facultad de Ciencias Médicas. Secretaria de Ciencia y Tecnología 2017-12-08 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf image/jpeg image/jpeg image/jpeg image/jpeg application/vnd.openxmlformats-officedocument.wordprocessingml.document https://revistas.unc.edu.ar/index.php/med/article/view/15361 10.31053/1853.0605.v74.n4.15361 Revista de la Facultad de Ciencias Médicas de Córdoba.; Vol. 74 No. 4 (2017); 325-330 Revista de la Facultad de Ciencias Médicas de Córdoba; Vol. 74 Núm. 4 (2017); 325-330 Revista da Faculdade de Ciências Médicas de Córdoba; v. 74 n. 4 (2017); 325-330 1853-0605 0014-6722 10.31053/1853.0605.v74.n4 spa https://revistas.unc.edu.ar/index.php/med/article/view/15361/19469 https://revistas.unc.edu.ar/index.php/med/article/view/15361/26218 https://revistas.unc.edu.ar/index.php/med/article/view/15361/26219 https://revistas.unc.edu.ar/index.php/med/article/view/15361/26220 https://revistas.unc.edu.ar/index.php/med/article/view/15361/26221 https://revistas.unc.edu.ar/index.php/med/article/view/15361/26222 Derechos de autor 2017 Universidad Nacional de Córdoba https://creativecommons.org/licenses/by-nc/4.0

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