Spore viability of fern species of the genera Amauropelta, Blechnum, and Physematium from central Argentina after low temperature storage
Background and aims: Ferns are sensitive to environmental changes, inhabiting ecosystems susceptible to degradation. Spore banks and in vitro spore culture are useful tools employed in their conservation. The objective was to test the ability of the spores to germinate after being stored in a freeze...
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| Formato: | Artículo revista |
| Lenguaje: | Inglés |
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Sociedad Argentina de Botánica
2024
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| Acceso en línea: | https://revistas.unc.edu.ar/index.php/BSAB/article/view/44658 |
| Aporte de: |
| id |
I10-R325-article-44658 |
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| record_format |
ojs |
| institution |
Universidad Nacional de Córdoba |
| institution_str |
I-10 |
| repository_str |
R-325 |
| container_title_str |
Boletín de la Sociedad Argentina de Botánica |
| language |
Inglés |
| format |
Artículo revista |
| topic |
Amauropelta Blechnum ex-situ conservation fern, freezing Physematium spore longevity Amauropelta Blechnum congelación conservación ex situ helecho longevidad de espora |
| spellingShingle |
Amauropelta Blechnum ex-situ conservation fern, freezing Physematium spore longevity Amauropelta Blechnum congelación conservación ex situ helecho longevidad de espora Martinenco, M. Luz Arana, Marcelo D. Luna, M. Lujan Luna, M. Lujan Spore viability of fern species of the genera Amauropelta, Blechnum, and Physematium from central Argentina after low temperature storage |
| topic_facet |
Amauropelta Blechnum ex-situ conservation fern, freezing Physematium spore longevity Amauropelta Blechnum congelación conservación ex situ helecho longevidad de espora |
| author |
Martinenco, M. Luz Arana, Marcelo D. Luna, M. Lujan Luna, M. Lujan |
| author_facet |
Martinenco, M. Luz Arana, Marcelo D. Luna, M. Lujan Luna, M. Lujan |
| author_sort |
Martinenco, M. Luz |
| title |
Spore viability of fern species of the genera Amauropelta, Blechnum, and Physematium from central Argentina after low temperature storage |
| title_short |
Spore viability of fern species of the genera Amauropelta, Blechnum, and Physematium from central Argentina after low temperature storage |
| title_full |
Spore viability of fern species of the genera Amauropelta, Blechnum, and Physematium from central Argentina after low temperature storage |
| title_fullStr |
Spore viability of fern species of the genera Amauropelta, Blechnum, and Physematium from central Argentina after low temperature storage |
| title_full_unstemmed |
Spore viability of fern species of the genera Amauropelta, Blechnum, and Physematium from central Argentina after low temperature storage |
| title_sort |
spore viability of fern species of the genera amauropelta, blechnum, and physematium from central argentina after low temperature storage |
| description |
Background and aims: Ferns are sensitive to environmental changes, inhabiting ecosystems susceptible to degradation. Spore banks and in vitro spore culture are useful tools employed in their conservation. The objective was to test the ability of the spores to germinate after being stored in a freezer and to develop gametophytes and sporophytes, in native fern species of genera Amauropelta, Blechnum and Physematium.
M&M: Spores of A. argentina, B. auriculatum and P. montevidensis were stored at -20 ºC in dry conditions for 6 and 12 months. They were sown in vitro in Dyer liquid medium and incubated in a growth chamber. The means of germination percentages were calculated and a one-way Student’s t-test was employed. Gametophyte and sporophyte development was registered under light and stereoscopic microscopes.
Results: Depending on the species, statistical differences were recorded in germination percentages (viability) between both storage periods. The spores of A. argentina kept in freezer for 12 months completely lost viability. In B. auriculatum, viability decreased over time, and in P. montevidensis it remained constant. Gametophytes and sporophytes developed in all cultures; except in A. argentina spores, with 12 months in freezer.
Conclusions: The viability of the spores under dry storage at low temperature could be conditioned by the ecological requirements of the species. Protocols used for spore storage and culture allowed obtaining gametophytes and sporophytes in trials. |
| publisher |
Sociedad Argentina de Botánica |
| publishDate |
2024 |
| url |
https://revistas.unc.edu.ar/index.php/BSAB/article/view/44658 |
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2025-02-05T22:20:42Z |
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2025-02-05T22:20:42Z |
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I10-R325-article-446582024-09-30T17:44:48Z Spore viability of fern species of the genera Amauropelta, Blechnum, and Physematium from central Argentina after low temperature storage Viabilidad de esporas de helechos de los géneros Amauropelta, Blechnum y Physematium del centro de Argentina luego del almacenamiento a baja temperatura Martinenco, M. Luz Arana, Marcelo D. Luna, M. Lujan Luna, M. Lujan Amauropelta Blechnum ex-situ conservation fern, freezing Physematium spore longevity Amauropelta Blechnum congelación conservación ex situ helecho longevidad de espora Background and aims: Ferns are sensitive to environmental changes, inhabiting ecosystems susceptible to degradation. Spore banks and in vitro spore culture are useful tools employed in their conservation. The objective was to test the ability of the spores to germinate after being stored in a freezer and to develop gametophytes and sporophytes, in native fern species of genera Amauropelta, Blechnum and Physematium. M&M: Spores of A. argentina, B. auriculatum and P. montevidensis were stored at -20 ºC in dry conditions for 6 and 12 months. They were sown in vitro in Dyer liquid medium and incubated in a growth chamber. The means of germination percentages were calculated and a one-way Student’s t-test was employed. Gametophyte and sporophyte development was registered under light and stereoscopic microscopes. Results: Depending on the species, statistical differences were recorded in germination percentages (viability) between both storage periods. The spores of A. argentina kept in freezer for 12 months completely lost viability. In B. auriculatum, viability decreased over time, and in P. montevidensis it remained constant. Gametophytes and sporophytes developed in all cultures; except in A. argentina spores, with 12 months in freezer. Conclusions: The viability of the spores under dry storage at low temperature could be conditioned by the ecological requirements of the species. Protocols used for spore storage and culture allowed obtaining gametophytes and sporophytes in trials. Introducción y objetivos: Los helechos son sensibles a cambios ambientales, habitando en ecosistemas susceptibles a degradación. Los bancos de esporas y el cultivo in vitro son herramientas útiles para su conservación. El objetivo fue probar la capacidad de las esporas para germinar luego del almacenamiento en freezer y de desarrollar gametofitos y esporofitos, en especies de helechos nativos de los géneros Amauropelta, Blechnum y Physematium. M&M: Esporas de A. argentina, B. auriculatum y P. montevidensis se almacenaron deshidratadas a -20 ºC durante 6 y 12 meses. Se sembraron in vitro en medio Dyer líquido y se incubaron en estufa. Se calcularon las medias de los porcentajes de germinación y se empleó una prueba t Student unidireccional. El desarrollo de gametofitos y esporofitos fue registrado con microscopio óptico y lupa. Resultados: Se registraron diferencias estadísticas en los porcentajes de germinación (viabilidad) entre ambos períodos de almacenamiento. Las esporas de A. argentina mantenidas durante 12 meses en freezer perdieron completamente la viabilidad. En B. auriculatum, la viabilidad disminuyó con el tiempo y en P. montevidensis que permaneció constante. Se desarrollaron gametofitos y esporofitos en todos los cultivos; excepto en A. argentina almacenadas 12 meses en freezer. Conclusiones: La viabilidad de esporas almacenadas deshidratadas a baja temperatura podría estar condicionada por los requerimientos ecológicos de las especies. Los protocolos utilizados para el almacenamiento y cultivo de esporas permitieron obtener gametofitos y esporofitos en todos los ensayos. Sociedad Argentina de Botánica 2024-09-30 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf text/html https://revistas.unc.edu.ar/index.php/BSAB/article/view/44658 10.31055/1851.2372.v59.n3.44658 Boletín de la Sociedad Argentina de Botánica (Journal of the Argentine Botanical Society; Vol. 59 No. 3 (2024): Numero Especial de Helechos Boletín de la Sociedad Argentina de Botánica; Vol. 59 Núm. 3 (2024): Numero Especial de Helechos Boletín de la Sociedad Argentina de Botánica; v. 59 n. 3 (2024): Numero Especial de Helechos 1851-2372 0373-580X 10.31055/1851.2372.v59.n3 eng https://revistas.unc.edu.ar/index.php/BSAB/article/view/44658/46646 https://revistas.unc.edu.ar/index.php/BSAB/article/view/44658/46647 Derechos de autor 2024 Maria L. Martinenco, Marcelo D. Arana, M. Lujan Luna https://creativecommons.org/licenses/by-nc-nd/4.0 |