Detection of arbuscular mycorrhizal fungi in the litter of different tree species: review of three clarification techniques.

Background and aims: Arbuscular mycorrhizal fungi (AMF) are obligate symbionts with plant roots, and they colonize the surrounding soil and leaf litter. A unique clarification technique has been used for studying AMF in decomposing leaves, but it is not optimal for all types. This study aimed to eva...

Descripción completa

Detalles Bibliográficos
Autores principales: Crescio, Sofía, Godeas, Alicia Margarita, Silvani, Vanesa
Formato: Artículo revista
Lenguaje:Español
Publicado: Sociedad Argentina de Botánica 2023
Materias:
clarificación
estructuras fúngicas
histoquímica
hojarasca
hongos micorrícicos arbusculares
arbuscular mycorrhizal fungi
clarification techniques
fungal structures
histochemistry
leaf litter
Acceso en línea:https://revistas.unc.edu.ar/index.php/BSAB/article/view/37810
Aporte de:
Boletín de la Sociedad Argentina de Botánica de Universidad Nacional de Córdoba
id I10-R325-article-37810
record_format ojs
institution Universidad Nacional de Córdoba
institution_str I-10
repository_str R-325
container_title_str Boletín de la Sociedad Argentina de Botánica
language Español
format Artículo revista
topic clarificación
estructuras fúngicas
histoquímica
hojarasca
hongos micorrícicos arbusculares
arbuscular mycorrhizal fungi
clarification techniques
fungal structures
histochemistry
leaf litter
spellingShingle clarificación
estructuras fúngicas
histoquímica
hojarasca
hongos micorrícicos arbusculares
arbuscular mycorrhizal fungi
clarification techniques
fungal structures
histochemistry
leaf litter
Crescio, Sofía
Godeas, Alicia Margarita
Silvani, Vanesa
Detection of arbuscular mycorrhizal fungi in the litter of different tree species: review of three clarification techniques.
topic_facet clarificación
estructuras fúngicas
histoquímica
hojarasca
hongos micorrícicos arbusculares
arbuscular mycorrhizal fungi
clarification techniques
fungal structures
histochemistry
leaf litter
author Crescio, Sofía
Godeas, Alicia Margarita
Silvani, Vanesa
author_facet Crescio, Sofía
Godeas, Alicia Margarita
Silvani, Vanesa
author_sort Crescio, Sofía
title Detection of arbuscular mycorrhizal fungi in the litter of different tree species: review of three clarification techniques.
title_short Detection of arbuscular mycorrhizal fungi in the litter of different tree species: review of three clarification techniques.
title_full Detection of arbuscular mycorrhizal fungi in the litter of different tree species: review of three clarification techniques.
title_fullStr Detection of arbuscular mycorrhizal fungi in the litter of different tree species: review of three clarification techniques.
title_full_unstemmed Detection of arbuscular mycorrhizal fungi in the litter of different tree species: review of three clarification techniques.
title_sort detection of arbuscular mycorrhizal fungi in the litter of different tree species: review of three clarification techniques.
description Background and aims: Arbuscular mycorrhizal fungi (AMF) are obligate symbionts with plant roots, and they colonize the surrounding soil and leaf litter. A unique clarification technique has been used for studying AMF in decomposing leaves, but it is not optimal for all types. This study aimed to evaluate and adjust three clearing techniques on leaf litter of diverse plant species for the detection of AMF structures.   M&M: We collected leaf litter of Quercus robur, Tipuana tipu, Ulmus minor, Fraxinus pennsylvanica, Platanus acerifolia, Laurus nobilis, Populus alba and Melia azedarach from soil surface of two parks in Buenos Aires (Argentina). Also, we inoculated leaf litter of F. pennsylvanica with Rhizoglomus intraradices in a pot culture under semi- controlled conditions. Three clarification techniques were tested: the “5-5-5 staining technique” by Arambarri, the root clarification technique by Phillips & Hayman, and Peterson et al. The time and temperature to reagent exposure were adjusted according to each decomposing leaf. Results: The clarification technique of Peterson et al. was the most appropriate for all the tested leaf litters. An effective clarification, conservation of leaf structure and visualization of extraradical spores and mycelia of AMF was achieved.   Conclusions: The selection of the appropriate technique greatly depends on the quality and composition of leaf litter. By the optimization of Peterson et al. Technique, we were able to detect structures of AMF on decomposing leaves of F. pennsylvanica and T. tipu.
publisher Sociedad Argentina de Botánica
publishDate 2023
url https://revistas.unc.edu.ar/index.php/BSAB/article/view/37810
work_keys_str_mv AT cresciosofia detectionofarbuscularmycorrhizalfungiinthelitterofdifferenttreespeciesreviewofthreeclarificationtechniques
AT godeasaliciamargarita detectionofarbuscularmycorrhizalfungiinthelitterofdifferenttreespeciesreviewofthreeclarificationtechniques
AT silvanivanesa detectionofarbuscularmycorrhizalfungiinthelitterofdifferenttreespeciesreviewofthreeclarificationtechniques
AT cresciosofia detecciondehongosmicorricicosarbuscularesenlahojarascadedistintasespeciesarboreasevaluaciondetrestecnicasdeclarificacion
AT godeasaliciamargarita detecciondehongosmicorricicosarbuscularesenlahojarascadedistintasespeciesarboreasevaluaciondetrestecnicasdeclarificacion
AT silvanivanesa detecciondehongosmicorricicosarbuscularesenlahojarascadedistintasespeciesarboreasevaluaciondetrestecnicasdeclarificacion
first_indexed 2024-09-03T22:50:19Z
last_indexed 2024-09-03T22:50:19Z
_version_ 1809217035839733760
spelling I10-R325-article-378102023-07-03T18:09:55Z Detection of arbuscular mycorrhizal fungi in the litter of different tree species: review of three clarification techniques. Detección de hongos micorrícicos arbusculares en la hojarasca de distintas especies arbóreas: evaluación de tres técnicas de clarificación. Crescio, Sofía Godeas, Alicia Margarita Silvani, Vanesa clarificación estructuras fúngicas histoquímica hojarasca hongos micorrícicos arbusculares arbuscular mycorrhizal fungi clarification techniques fungal structures histochemistry leaf litter Background and aims: Arbuscular mycorrhizal fungi (AMF) are obligate symbionts with plant roots, and they colonize the surrounding soil and leaf litter. A unique clarification technique has been used for studying AMF in decomposing leaves, but it is not optimal for all types. This study aimed to evaluate and adjust three clearing techniques on leaf litter of diverse plant species for the detection of AMF structures.   M&M: We collected leaf litter of Quercus robur, Tipuana tipu, Ulmus minor, Fraxinus pennsylvanica, Platanus acerifolia, Laurus nobilis, Populus alba and Melia azedarach from soil surface of two parks in Buenos Aires (Argentina). Also, we inoculated leaf litter of F. pennsylvanica with Rhizoglomus intraradices in a pot culture under semi- controlled conditions. Three clarification techniques were tested: the “5-5-5 staining technique” by Arambarri, the root clarification technique by Phillips & Hayman, and Peterson et al. The time and temperature to reagent exposure were adjusted according to each decomposing leaf. Results: The clarification technique of Peterson et al. was the most appropriate for all the tested leaf litters. An effective clarification, conservation of leaf structure and visualization of extraradical spores and mycelia of AMF was achieved.   Conclusions: The selection of the appropriate technique greatly depends on the quality and composition of leaf litter. By the optimization of Peterson et al. Technique, we were able to detect structures of AMF on decomposing leaves of F. pennsylvanica and T. tipu. Introducción y objetivos: Los hongos micorrícicos arbusculares (HMA) forman asociaciones simbióticas mutualistas con las raíces de las plantas y se desarrollan en el suelo y hojarasca. Hasta el momento, se ha empleado una única técnica de clarificación en hojarasca para el estudio de los HMA, pero dada la gran variabilidad anatómica y bioquímica de las hojas, es necesario analizar otras metodologías. En este trabajo se evaluaron y ajustaron tres técnicas de clarificación en hojarasca de distintas especies arbóreas para la detección de HMA. M&M: Se recolectó hojarasca de Quercus robur, Tipuana tipu, Ulmus minor, Fraxinus pennsylvanica, Platanus acerifolia, Laurus nobilis, Populus alba and Melia azedarach de la superficie del suelo en dos parques de la ciudad de Buenos Aires. Además, se inoculó hojarasca de F. pennsylvanica con Rhizoglomus intraradices en un cultivo bajo condiciones semicontroladas. Fueron evaluadas tres técnicas de clarificación: “5 – 5 – 5” de Arambarri (2018), Phillips & Hayman (1970) y Peterson et al. (2008), y se ajustaron modificando la duración y condiciones de exposición a los reactivos. Resultados: La técnica de clarificación propuesta por Peterson et al. (2008) fue la más apropiada para todas las hojarascas evaluadas, dado que se obtuvo una eficaz clarificación, conservación de la estructura foliar y la visualización de esporas e hifas de HMA. Conclusión: La selección adecuada de la técnica depende de la calidad de la hojarasca. La optimización del método de Peterson et al. (2008) permitió detectar estructuras de HMA en la hojarasca delicada de F. pennsylvanica y T. tipu. Sociedad Argentina de Botánica 2023-03-31 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf text/html https://revistas.unc.edu.ar/index.php/BSAB/article/view/37810 10.31055/1851.2372.v58.n2.37810 Boletín de la Sociedad Argentina de Botánica (Journal of the Argentine Botanical Society; Vol. 58 No. 2 (2023): June Boletín de la Sociedad Argentina de Botánica; Vol. 58 Núm. 2 (2023): Junio Boletín de la Sociedad Argentina de Botánica; v. 58 n. 2 (2023): Junio 1851-2372 0373-580X 10.31055/1851.2372.v58.n2 spa https://revistas.unc.edu.ar/index.php/BSAB/article/view/37810/40947 https://revistas.unc.edu.ar/index.php/BSAB/article/view/37810/40948 Derechos de autor 2023 Sofía Crescio, Alicia Margarita Godeas, Vanesa Silvani https://creativecommons.org/licenses/by-nc-nd/4.0

Ejemplares similares