An easy technique for silicophytolith visualization in plants through tissue clearing and immersion oil mounting

Background and aims: Different methodologies were proposed for the detection of silica deposits (silicophytoliths) in plant tissues. These methodologies include dry and wet ashing (which destroy the surrounding tissue), phenol staining (which is toxic), safranin–crystal violet lactone and fast green...

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Autores principales: Fernández Honaine, Mariana, Benvenuto, María Laura, Osterrieth, Margarita L.
Formato: Artículo revista
Lenguaje:Inglés
Español
Publicado: Sociedad Argentina de Botánica 2019
Materias:
anatomy
dicotyledons
Equisetum sp
immersion oil
monocotyledons
silicophytoliths
tissue clearing
aceite de inmersión
anatomía
clarificación
dicotiledóneas
monocotiledóneas
silicofitolitos
Acceso en línea:https://revistas.unc.edu.ar/index.php/BSAB/article/view/25359
Aporte de:
Boletín de la Sociedad Argentina de Botánica de Universidad Nacional de Córdoba
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institution Universidad Nacional de Córdoba
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container_title_str Boletín de la Sociedad Argentina de Botánica
language Inglés
Español
format Artículo revista
topic anatomy
dicotyledons
Equisetum sp
immersion oil
monocotyledons
silicophytoliths
tissue clearing
aceite de inmersión
anatomía
clarificación
dicotiledóneas
Equisetum sp
monocotiledóneas
silicofitolitos
spellingShingle anatomy
dicotyledons
Equisetum sp
immersion oil
monocotyledons
silicophytoliths
tissue clearing
aceite de inmersión
anatomía
clarificación
dicotiledóneas
Equisetum sp
monocotiledóneas
silicofitolitos
Fernández Honaine, Mariana
Benvenuto, María Laura
Osterrieth, Margarita L.
An easy technique for silicophytolith visualization in plants through tissue clearing and immersion oil mounting
topic_facet anatomy
dicotyledons
Equisetum sp
immersion oil
monocotyledons
silicophytoliths
tissue clearing
aceite de inmersión
anatomía
clarificación
dicotiledóneas
Equisetum sp
monocotiledóneas
silicofitolitos
author Fernández Honaine, Mariana
Benvenuto, María Laura
Osterrieth, Margarita L.
author_facet Fernández Honaine, Mariana
Benvenuto, María Laura
Osterrieth, Margarita L.
author_sort Fernández Honaine, Mariana
title An easy technique for silicophytolith visualization in plants through tissue clearing and immersion oil mounting
title_short An easy technique for silicophytolith visualization in plants through tissue clearing and immersion oil mounting
title_full An easy technique for silicophytolith visualization in plants through tissue clearing and immersion oil mounting
title_fullStr An easy technique for silicophytolith visualization in plants through tissue clearing and immersion oil mounting
title_full_unstemmed An easy technique for silicophytolith visualization in plants through tissue clearing and immersion oil mounting
title_sort easy technique for silicophytolith visualization in plants through tissue clearing and immersion oil mounting
description Background and aims: Different methodologies were proposed for the detection of silica deposits (silicophytoliths) in plant tissues. These methodologies include dry and wet ashing (which destroy the surrounding tissue), phenol staining (which is toxic), safranin–crystal violet lactone and fast green–methyl red staining (not specific for silica), fluorescence microscopy, electronic microscopy, EDAX and Raman analyses (which involve expensive equipment). Here we presented an easy and cheap method based on tissue clearing and immersion oil mounting. M&M: We tested the methodology in longitudinal and cross sections of culms, leaves and roots of ten species that effectively accumulate silica. We applied different clearing techniques according to the type of plant material, we mounted in immersion oil and observed under light microscope. We compared the results with the ones obtained by traditional silicophytolith techniques (dry ashing, phenol staining and SEM-EDAX analyses). Results: Silica deposits were observed in all species and organs analyzed, and the observations were coincident with the results obtained by other techniques. It was also possible to identify calcium crystals, allowing the description of the most common biomineralizations produced by plants. Conclusions: The technique here proposed can be used for exploratory as well as for specific studies about the content and distribution of silicophytoliths in almost any tissue, organ and plant species. It can be applied in any laboratory, because it does not require expensive or hardly available equipment.
publisher Sociedad Argentina de Botánica
publishDate 2019
url https://revistas.unc.edu.ar/index.php/BSAB/article/view/25359
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first_indexed 2024-09-03T22:49:24Z
last_indexed 2024-09-03T22:49:24Z
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spelling I10-R325-article-253592019-12-14T13:45:28Z An easy technique for silicophytolith visualization in plants through tissue clearing and immersion oil mounting Una técnica sencilla para la visualización de silicofitolitos en plantas mediante clarificación y montaje en aceite de inmersión Fernández Honaine, Mariana Benvenuto, María Laura Osterrieth, Margarita L. anatomy dicotyledons Equisetum sp immersion oil monocotyledons silicophytoliths tissue clearing aceite de inmersión anatomía clarificación dicotiledóneas Equisetum sp monocotiledóneas silicofitolitos Background and aims: Different methodologies were proposed for the detection of silica deposits (silicophytoliths) in plant tissues. These methodologies include dry and wet ashing (which destroy the surrounding tissue), phenol staining (which is toxic), safranin–crystal violet lactone and fast green–methyl red staining (not specific for silica), fluorescence microscopy, electronic microscopy, EDAX and Raman analyses (which involve expensive equipment). Here we presented an easy and cheap method based on tissue clearing and immersion oil mounting. M&M: We tested the methodology in longitudinal and cross sections of culms, leaves and roots of ten species that effectively accumulate silica. We applied different clearing techniques according to the type of plant material, we mounted in immersion oil and observed under light microscope. We compared the results with the ones obtained by traditional silicophytolith techniques (dry ashing, phenol staining and SEM-EDAX analyses). Results: Silica deposits were observed in all species and organs analyzed, and the observations were coincident with the results obtained by other techniques. It was also possible to identify calcium crystals, allowing the description of the most common biomineralizations produced by plants. Conclusions: The technique here proposed can be used for exploratory as well as for specific studies about the content and distribution of silicophytoliths in almost any tissue, organ and plant species. It can be applied in any laboratory, because it does not require expensive or hardly available equipment. Introducción y objetivos: Diversas metodologías han sido propuestas para la identificación de depósitos de sílice (silicofitolitos) en los tejidos vegetales. Estas metodologías incluyen calcinaciones y digestiones químicas (que destruyen el tejido que contiene a los silicofitolitos); tinción con fenol (el cual es tóxico); tinciones con safranina-cristal violeta y fast green-rojo metilo (las cuales no resultan específicas para la sílice); microscopía de fluorescencia, microscopía electrónica, EDAX y espectroscopía Raman (las cuales incluyen equipamiento costoso). En este trabajo se presenta un método económico y sencillo basado en la clarificación de los tejidos y su montaje en aceite de inmersión. M&M: Testeamos el método en cortes longitudinales y transversales de hojas, tallos y raíces de diez especies que acumulan sílice. Aplicamos diferentes técnicas de clarificación de acuerdo al tipo de material, montamos en aceite de inmersión y observamos al microscopio óptico. Los resultados se compararon con los obtenidos por las técnicas tradicionales de silicofitolitos (calcinaciones, tinción con fenol y análisis en MEB-EDAX). Resultados: Se observaron depósitos de sílice en todas las especies y órganos analizados, y éstos coinciden con los resultados obtenidos por las técnicas tradicionales. Asimismo, mediante esta técnica, fue posible identificar cristales de calcio, permitiendo la descripción de los dos tipos más comunes de biomineralizaciones en plantas. Conclusiones: La técnica propuesta puede ser usada para estudios exploratorios, como así específicos, sobre el contenido y distribución de silicofitolitos en casi cualquier tipo de tejido, órganos y especie. Puede ser aplicado en cualquier laboratorio, debido a que no requiere de equipamiento costoso. Sociedad Argentina de Botánica 2019-09-24 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Artículo original application/pdf text/html https://revistas.unc.edu.ar/index.php/BSAB/article/view/25359 10.31055/1851.2372.v54.n3.25359 Boletín de la Sociedad Argentina de Botánica (Journal of the Argentine Botanical Society; Vol. 54 No. 3 (2019): September; 353-365 Boletín de la Sociedad Argentina de Botánica; Vol. 54 Núm. 3 (2019): Septiembre; 353-365 Boletín de la Sociedad Argentina de Botánica; v. 54 n. 3 (2019): Septiembre; 353-365 1851-2372 0373-580X 10.31055/1851.2372.v54.n3 eng spa https://revistas.unc.edu.ar/index.php/BSAB/article/view/25359/24733 https://revistas.unc.edu.ar/index.php/BSAB/article/view/25359/27437 Derechos de autor 2019 Boletín de la Sociedad Argentina de Botánica

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