Determination of mercury in vegetal tissues by microPIXE: Application to the study of hyperaccumulation by Spirodela intermedia (Lemnaceae)
Background and aims: Aqueous mercury (II), Hg2+, is still nowadays a hazardous pollutant with a large dispersion. Phytoremediation strategies are an environmental friendly and low-cost alternative. In order to improve these processes, Spirodela intermedia, an autochthonous floating macrophyte, was u...
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| Formato: | Artículo revista |
| Lenguaje: | Inglés Español |
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Sociedad Argentina de Botánica
2019
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| Materias: | |
| Acceso en línea: | https://revistas.unc.edu.ar/index.php/BSAB/article/view/24373 |
| Aporte de: |
| id |
I10-R325-article-24373 |
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| record_format |
ojs |
| institution |
Universidad Nacional de Córdoba |
| institution_str |
I-10 |
| repository_str |
R-325 |
| container_title_str |
Boletín de la Sociedad Argentina de Botánica |
| language |
Inglés Español |
| format |
Artículo revista |
| topic |
Conservation area lichens Pyxine taxonomy. Spirodela intermedia mercurio hiperacumulación microPIXE. |
| spellingShingle |
Conservation area lichens Pyxine taxonomy. Spirodela intermedia mercurio hiperacumulación microPIXE. De La Fournière, Emmanuel M. Vega, Nahuel A. Müller, Nahuel A. Pizarro, Ramón A. Debray, Mario E. Determination of mercury in vegetal tissues by microPIXE: Application to the study of hyperaccumulation by Spirodela intermedia (Lemnaceae) |
| topic_facet |
Conservation area lichens Pyxine taxonomy. Spirodela intermedia mercurio hiperacumulación microPIXE. |
| author |
De La Fournière, Emmanuel M. Vega, Nahuel A. Müller, Nahuel A. Pizarro, Ramón A. Debray, Mario E. |
| author_facet |
De La Fournière, Emmanuel M. Vega, Nahuel A. Müller, Nahuel A. Pizarro, Ramón A. Debray, Mario E. |
| author_sort |
De La Fournière, Emmanuel M. |
| title |
Determination of mercury in vegetal tissues by microPIXE: Application to the study of hyperaccumulation by Spirodela intermedia (Lemnaceae) |
| title_short |
Determination of mercury in vegetal tissues by microPIXE: Application to the study of hyperaccumulation by Spirodela intermedia (Lemnaceae) |
| title_full |
Determination of mercury in vegetal tissues by microPIXE: Application to the study of hyperaccumulation by Spirodela intermedia (Lemnaceae) |
| title_fullStr |
Determination of mercury in vegetal tissues by microPIXE: Application to the study of hyperaccumulation by Spirodela intermedia (Lemnaceae) |
| title_full_unstemmed |
Determination of mercury in vegetal tissues by microPIXE: Application to the study of hyperaccumulation by Spirodela intermedia (Lemnaceae) |
| title_sort |
determination of mercury in vegetal tissues by micropixe: application to the study of hyperaccumulation by spirodela intermedia (lemnaceae) |
| description |
Background and aims: Aqueous mercury (II), Hg2+, is still nowadays a hazardous pollutant with a large dispersion. Phytoremediation strategies are an environmental friendly and low-cost alternative. In order to improve these processes, Spirodela intermedia, an autochthonous floating macrophyte, was used to remove Hg2+ from mineral water under laboratory conditions, studying the in vivo distribution of mercury and other elements by nuclear microprobe scanning mapping.
M&M: Exposures (1 and 10 mg.L-1 Hg2+ concentrations) were performed during at least 2 weeks. All the parameters from the bioremediation process as uptake rate, bioconcentration factors (BCFs) of mercury in roots and leaves and translocation factors (TFs), were achieved from microPIXE quantifications at Buenos Aires Tandar accelerator.
Results: For 1 and 10 mg.L-1 concentrations, S. intermedia can be considered as a hyperaccumulator. The highest BCFs (> 1000 in roots and >were measured, indicating that Hg2+ translocation is not taking place. High resolution spatial 2D maps of the in vivo distribution for different exposure conditions were established. It was observed that Hg2+ distribution in leaves is more heterogeneous than in roots. An important finding was the detection of Hg in chlorenchyma where its effects are more toxic. Correlation between mercury and calcium distribution and its relationship with physiological responses to intoxication have been examined.Conclusions: Phytoremediation of Hg2+ by S. intermedia is a convenient alternative. Since the protocol was performed using a real water, it becomes an advisable tool at higher scale. |
| publisher |
Sociedad Argentina de Botánica |
| publishDate |
2019 |
| url |
https://revistas.unc.edu.ar/index.php/BSAB/article/view/24373 |
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2024-09-03T22:49:21Z |
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2024-09-03T22:49:21Z |
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I10-R325-article-243732019-12-14T13:45:32Z Determination of mercury in vegetal tissues by microPIXE: Application to the study of hyperaccumulation by Spirodela intermedia (Lemnaceae) Determinación del mercurio en tejidos vegetales por microPIXE: Aplicación al estudio de la hiperacumulación por Spirodela intermedia (Lemnaceae). De La Fournière, Emmanuel M. Vega, Nahuel A. Müller, Nahuel A. Pizarro, Ramón A. Debray, Mario E. Conservation area lichens Pyxine taxonomy. Spirodela intermedia mercurio hiperacumulación microPIXE. Background and aims: Aqueous mercury (II), Hg2+, is still nowadays a hazardous pollutant with a large dispersion. Phytoremediation strategies are an environmental friendly and low-cost alternative. In order to improve these processes, Spirodela intermedia, an autochthonous floating macrophyte, was used to remove Hg2+ from mineral water under laboratory conditions, studying the in vivo distribution of mercury and other elements by nuclear microprobe scanning mapping. M&M: Exposures (1 and 10 mg.L-1 Hg2+ concentrations) were performed during at least 2 weeks. All the parameters from the bioremediation process as uptake rate, bioconcentration factors (BCFs) of mercury in roots and leaves and translocation factors (TFs), were achieved from microPIXE quantifications at Buenos Aires Tandar accelerator. Results: For 1 and 10 mg.L-1 concentrations, S. intermedia can be considered as a hyperaccumulator. The highest BCFs (> 1000 in roots and >were measured, indicating that Hg2+ translocation is not taking place. High resolution spatial 2D maps of the in vivo distribution for different exposure conditions were established. It was observed that Hg2+ distribution in leaves is more heterogeneous than in roots. An important finding was the detection of Hg in chlorenchyma where its effects are more toxic. Correlation between mercury and calcium distribution and its relationship with physiological responses to intoxication have been examined.Conclusions: Phytoremediation of Hg2+ by S. intermedia is a convenient alternative. Since the protocol was performed using a real water, it becomes an advisable tool at higher scale. Introducción y objetivos: El mercurio (II) acuoso, Hg2+, es todavía un contaminante peligroso ampliamente distribuido. Las estrategias de fitorremediación son ambientalmente amigables y de bajo costo. Con el fin de optimizar estos procesos, se utilizó Spirodela intermedia, una macrófita acuática autóctona, para remover Hg2+ en agua mineral, en condiciones de laboratorio, estudiando la distribución in vivo de mercurio y otros elementos por mapeo barriendo con una microsonda nuclear.M&M: Las exposiciones (concentraciones de Hg2+ de 1 y 10 mg.L-1) duraron al menos 2 semanas. Los parámetros: tasa de captación, factores de bioconcentración (BCFs) de mercurio en raíces y frondes y factores de translocación (TFs) fueron calculados a partir de cuantificaciones de microPIXE con el acelerador Tandar de Buenos Aires.Resultados: S. intermedia puede ser considerado un hiperacumulador. Los más altos BCFs (> 1000 raíces y > 200 frondes) correspondieron a 1 mg.L-1 a las 96 hs. En todos los casos, se constató que TFs < 1, indicando que no ocurre translocación de Hg2+. Se obtuvieron mapas 2D de alta resolución espacial de la distribución elemental in vivo para las diferentes condiciones. Se observó que la distribución de mercurio en frondes es más heterogénea que en raíces. Fue importante la detección de Hg en clorénquima donde sus efectos son más tóxicos. Se analizó una correlación entre la distribución de mercurio y calcio y la relación con respuestas fisiológicas.Conclusiones: La fitorremediación de Hg2+ con S. intermedia es una alternativa conveniente. Por haberse realizado en agua real, el protocolo es escalable. Sociedad Argentina de Botánica 2019-06-20 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Artículo original application/pdf text/html https://revistas.unc.edu.ar/index.php/BSAB/article/view/24373 10.31055/1851.2372.v54.n2.24373 Boletín de la Sociedad Argentina de Botánica (Journal of the Argentine Botanical Society; Vol. 54 No. 2 (2019): June; 263-275 Boletín de la Sociedad Argentina de Botánica; Vol. 54 Núm. 2 (2019): Junio; 263-275 Boletín de la Sociedad Argentina de Botánica; v. 54 n. 2 (2019): Junio; 263-275 1851-2372 0373-580X 10.31055/1851.2372.v54.n2 eng spa https://revistas.unc.edu.ar/index.php/BSAB/article/view/24373/23703 https://revistas.unc.edu.ar/index.php/BSAB/article/view/24373/27453 Derechos de autor 2019 Boletín de la Sociedad Argentina de Botánica |