Distribution of pectins in the pollen apertures of Oenothera hookeri.velans ster/+ster.

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Cell wall pectins are some of the most complex biopolymers known, and yet their functions remain largely mysterious. The aim of this paper was to deepen the study of the spatial pattern of pectin distribution in the aperture of Oenothera hookeri.velans ster/+ster fertile pollen. We used “in situ” im...

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Detalles Bibliográficos
Autores principales: Rodriguez García, M.I., Famá, G., Cismondi, I.A., Noher de Halac, I.
Formato: article
Lenguaje:Inglés
Publicado: Biocell : official journal of the Sociedades Latinoamericanas de Microscopía Electronica ... et. al 2017
Materias:
Pectins
Antibodies Monoclonal
Immunohistochemistry
Pollen
Oenothera
Histocytochemistry
Acceso en línea:http://hdl.handle.net/11086/4911
Aporte de:
Repositorio Digital Universitario (UNC) de Universidad Nacional de Córdoba
id I10-R14111086-4911
record_format dspace
institution Universidad Nacional de Córdoba
institution_str I-10
repository_str R-141
collection Repositorio Digital Universitario (UNC)
language Inglés
topic Pectins
Antibodies Monoclonal
Immunohistochemistry
Pollen
Oenothera
Histocytochemistry
spellingShingle Pectins
Antibodies Monoclonal
Immunohistochemistry
Pollen
Oenothera
Histocytochemistry
Rodriguez García, M.I.
Famá, G.
Cismondi, I.A.
Noher de Halac, I.
Distribution of pectins in the pollen apertures of Oenothera hookeri.velans ster/+ster.
topic_facet Pectins
Antibodies Monoclonal
Immunohistochemistry
Pollen
Oenothera
Histocytochemistry
description Cell wall pectins are some of the most complex biopolymers known, and yet their functions remain largely mysterious. The aim of this paper was to deepen the study of the spatial pattern of pectin distribution in the aperture of Oenothera hookeri.velans ster/+ster fertile pollen. We used “in situ” immunocytochemical techniques at electron microscopy, involving monoclonal antibodies JIM5 and JIM7 directed against pectin epitopes in fertile pollen grains of Oenothera hookeri.velans ster/+ster. The same region was also analyzed by classical cytochemistry for polysaccharide detection. Immunogold labelling at the JIM7 epitope showed only in mature pollen labelling mainly located at the intine endo-aperture region. Cytoplasmic structures near the plasma membrane of the vegetative cell showed no labelling gold grains. In the same pollen stage the labelling at the JIM5 epitope was mostly confined to a layer located in the limit between the endexine and the ektexine at the level of the border of the oncus. Some tubuli at the base of the ektexine showed also an accumulation of gold particles. No JIM5 label was demonstrated in the aperture chamber and either in any cytoplasmic structure of the pollen grains. The immunocytochemical technique, when compared with the traditional methods for non- cellulose polysaccharide cytochemistry is fare more sensitive and allows the univocal determination of temporal and spatial location of pectins recognized by the JIM7 and JIM5 MAbs.
format article
author Rodriguez García, M.I.
Famá, G.
Cismondi, I.A.
Noher de Halac, I.
author_facet Rodriguez García, M.I.
Famá, G.
Cismondi, I.A.
Noher de Halac, I.
author_sort Rodriguez García, M.I.
title Distribution of pectins in the pollen apertures of Oenothera hookeri.velans ster/+ster.
title_short Distribution of pectins in the pollen apertures of Oenothera hookeri.velans ster/+ster.
title_full Distribution of pectins in the pollen apertures of Oenothera hookeri.velans ster/+ster.
title_fullStr Distribution of pectins in the pollen apertures of Oenothera hookeri.velans ster/+ster.
title_full_unstemmed Distribution of pectins in the pollen apertures of Oenothera hookeri.velans ster/+ster.
title_sort distribution of pectins in the pollen apertures of oenothera hookeri.velans ster/+ster.
publisher Biocell : official journal of the Sociedades Latinoamericanas de Microscopía Electronica ... et. al
publishDate 2017
url http://hdl.handle.net/11086/4911
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