The extent of infectious SARS-CoV-2 shedding in an Argentinean cohort
Background: To analyze the infectious extent of severe acute respiratory syndrome coronavirus 2 (SARS-COV-2) in different settings where prevention strategies are critical to limit infection spread, we evaluated SARS-COV-2 viability to guide public health policies regarding isolation criteria and in...
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| Autores principales: | , , , , , , , , , |
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| Formato: | article |
| Lenguaje: | Inglés |
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2021
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| Acceso en línea: | http://hdl.handle.net/11086/20033 https://academic.oup.com/jpubhealth/advance-article/doi/10.1093/pubmed/fdab145/6272006?searchresult=1 |
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I10-R14111086-20033 |
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dspace |
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Universidad Nacional de Córdoba |
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I-10 |
| repository_str |
R-141 |
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Repositorio Digital Universitario (UNC) |
| language |
Inglés |
| topic |
Infectivity Isolation Covid 19 SARS-CoV-2 Public health Polymerase chain Reaction Reverse transcriptase polymerase chain reaction Infections Public health medicine Viruses Pathogenicity Viral isolation |
| spellingShingle |
Infectivity Isolation Covid 19 SARS-CoV-2 Public health Polymerase chain Reaction Reverse transcriptase polymerase chain reaction Infections Public health medicine Viruses Pathogenicity Viral isolation Blanco, Sebastián Aguilar, Juan Javier Konigheim, Brenda Salomé Diaz, Luis Adrián Spinsanti, Lorena Beranek, Mauricio Collino, César Diaz, Miguel Barbás, María Gabriela Gallego, Sandra Verónica The extent of infectious SARS-CoV-2 shedding in an Argentinean cohort |
| topic_facet |
Infectivity Isolation Covid 19 SARS-CoV-2 Public health Polymerase chain Reaction Reverse transcriptase polymerase chain reaction Infections Public health medicine Viruses Pathogenicity Viral isolation |
| description |
Background: To analyze the infectious extent of severe acute respiratory syndrome coronavirus 2 (SARS-COV-2) in different settings where prevention strategies are critical to limit infection spread, we evaluated SARS-COV-2 viability to guide public health policies regarding isolation criteria and infection control.
Methods: We attempted viral isolation in 82 nasopharyngeal swabs from 72 patients with con rmed SARS-COV-2 infection. Study population was divided into four groups: (i) Patients during the rst week of symptoms; (ii) Patients with prolonged positive PCR; (iii) Healthcare workers from a hospital participating of an outbreak investigation, with SARS-COV-2 infection con rmed by reverse transcriptase polymerase chain reaction (RT-PCR) and (iv) Recipients of convalescent immune plasma (CIP). Vero Cl76 cell-line (ATCC CRL-587) was used in assays for virus isolation. Plasma samples of CIP recipients were also tested with plaque-reduction neutralization test.
Results: We obtained infectious SARS-COV-2 isolates from 15/84 nasopharyngeal swabs. The virus could not be isolated from upper respiratory tract samples collected 10-day after onset of symptoms (AOS) in patients with mild–moderate disease.
Conclusion: The knowledge of the extent of SARS-CoV-2 infectivity AOS is relevant for effective prevention measures. This allows to discuss criteria for end isolation despite persistence of positive PCR and improve timing for hospital discharge with consequent availability of critical beds. |
| format |
article |
| author |
Blanco, Sebastián Aguilar, Juan Javier Konigheim, Brenda Salomé Diaz, Luis Adrián Spinsanti, Lorena Beranek, Mauricio Collino, César Diaz, Miguel Barbás, María Gabriela Gallego, Sandra Verónica |
| author_facet |
Blanco, Sebastián Aguilar, Juan Javier Konigheim, Brenda Salomé Diaz, Luis Adrián Spinsanti, Lorena Beranek, Mauricio Collino, César Diaz, Miguel Barbás, María Gabriela Gallego, Sandra Verónica |
| author_sort |
Blanco, Sebastián |
| title |
The extent of infectious SARS-CoV-2 shedding in an Argentinean cohort |
| title_short |
The extent of infectious SARS-CoV-2 shedding in an Argentinean cohort |
| title_full |
The extent of infectious SARS-CoV-2 shedding in an Argentinean cohort |
| title_fullStr |
The extent of infectious SARS-CoV-2 shedding in an Argentinean cohort |
| title_full_unstemmed |
The extent of infectious SARS-CoV-2 shedding in an Argentinean cohort |
| title_sort |
extent of infectious sars-cov-2 shedding in an argentinean cohort |
| publishDate |
2021 |
| url |
http://hdl.handle.net/11086/20033 https://academic.oup.com/jpubhealth/advance-article/doi/10.1093/pubmed/fdab145/6272006?searchresult=1 |
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