Octopamine is required for successful reproduction in the classical insect model, Rhodnius prolixus
Impact Factor (IF) 2023 (2024 update): 2.9. Revisado por pares
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Wolfgang Blenau, University of Leipzig. Faculty of Life Sciences: Universitat Leipzig Fakultat fur Lebenswissenschaften, GERMANY
2024
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Acceso en línea: | http://hdl.handle.net/11086/552763 https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0306611 https://pubmed.ncbi.nlm.nih.gov/38995904/ http://doi.org/10.1371/journal.pone.0306611 |
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I10-R141-11086-5527632024-07-31T12:23:43Z Octopamine is required for successful reproduction in the classical insect model, Rhodnius prolixus Leyria, Jimena Orchard, Ian Lange, Angela B. https://orcid.org/0000-0002-9145-5523 https://orcid.org/0000-0002-4491-7924 https://orcid.org/0000-0003-2195-4316 Biogenic amines Reproduction Octopamine (OA) RNA interference (RNAi) Rhodnius prolixus Impact Factor (IF) 2023 (2024 update): 2.9. Revisado por pares info:eu-repo/semantics/publishedVersion Fil: Leyria, Jimena. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Bioquímica Clínica; Argentina. Fil: Leyria, Jimena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina. Fil: Orchard, Ian. University of Toronto Mississauga, Department of Biology; Canada. Fil: Lange, Angela B. University of Toronto Mississauga, Department of Biology; Canada. In insects, biogenic amines function as neurotransmitters, neuromodulators, and neurohormones, influencing various behaviors, including those related to reproduction such as response to sex pheromones, oogenesis, oviposition, courtship, and mating. Octopamine (OA), an analog of the vertebrate norepinephrine, is synthesized from the biogenic amine tyramine by the enzyme tyramine β-hydroxylase (TβH). Here, we investigate the mechanisms and target genes underlying the role of OA in successful reproduction in females of Rhodnius prolixus, a vector of Chagas disease, by downregulating TβH mRNA expression (thereby reducing OA content) using RNA interference (RNAi), and in vivo and ex vivo application of OA. Injection of females with dsTβH impairs successful reproduction at least in part, by decreasing the transcript expression of enzymes involved in juvenile hormone biosynthesis, the primary hormone for oogenesis in R. prolixus, thereby interfering with oogenesis, ovulation and oviposition. This study offers valuable insights into the involvement of OA for successful reproduction in R. prolixus females. Understanding the reproductive biology of R. prolixus is crucial in a medical context for controlling the spread of the disease. info:eu-repo/semantics/publishedVersion Fil: Leyria, Jimena. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Bioquímica Clínica; Argentina. Fil: Leyria, Jimena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina. Fil: Orchard, Ian. University of Toronto Mississauga, Department of Biology; Canada. Fil: Lange, Angela B. University of Toronto Mississauga, Department of Biology; Canada. 2024-07-17T23:03:07Z 2024-07-17T23:03:07Z 2024-07-12 article Leyria J, Orchard I, Lange AB (2024) Octopamine is required for successful reproduction in the classical insect model, Rhodnius prolixus. PLoS ONE 19(7): e0306611. https://doi.org/ 10.1371/journal.pone.0306611 http://hdl.handle.net/11086/552763 1932-6203 https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0306611 https://pubmed.ncbi.nlm.nih.gov/38995904/ http://doi.org/10.1371/journal.pone.0306611 eng Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/ Wolfgang Blenau, University of Leipzig. Faculty of Life Sciences: Universitat Leipzig Fakultat fur Lebenswissenschaften, GERMANY |
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Universidad Nacional de Córdoba |
institution_str |
I-10 |
repository_str |
R-141 |
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Repositorio Digital Universitario (UNC) |
language |
Inglés |
topic |
Biogenic amines Reproduction Octopamine (OA) RNA interference (RNAi) Rhodnius prolixus |
spellingShingle |
Biogenic amines Reproduction Octopamine (OA) RNA interference (RNAi) Rhodnius prolixus Leyria, Jimena Orchard, Ian Lange, Angela B. Octopamine is required for successful reproduction in the classical insect model, Rhodnius prolixus |
topic_facet |
Biogenic amines Reproduction Octopamine (OA) RNA interference (RNAi) Rhodnius prolixus |
description |
Impact Factor (IF) 2023 (2024 update): 2.9. Revisado por pares |
author2 |
https://orcid.org/0000-0002-9145-5523 |
author_facet |
https://orcid.org/0000-0002-9145-5523 Leyria, Jimena Orchard, Ian Lange, Angela B. |
format |
publishedVersion article |
author |
Leyria, Jimena Orchard, Ian Lange, Angela B. |
author_sort |
Leyria, Jimena |
title |
Octopamine is required for successful reproduction in the classical insect model, Rhodnius prolixus |
title_short |
Octopamine is required for successful reproduction in the classical insect model, Rhodnius prolixus |
title_full |
Octopamine is required for successful reproduction in the classical insect model, Rhodnius prolixus |
title_fullStr |
Octopamine is required for successful reproduction in the classical insect model, Rhodnius prolixus |
title_full_unstemmed |
Octopamine is required for successful reproduction in the classical insect model, Rhodnius prolixus |
title_sort |
octopamine is required for successful reproduction in the classical insect model, rhodnius prolixus |
publisher |
Wolfgang Blenau, University of Leipzig. Faculty of Life Sciences: Universitat Leipzig Fakultat fur Lebenswissenschaften, GERMANY |
publishDate |
2024 |
url |
http://hdl.handle.net/11086/552763 https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0306611 https://pubmed.ncbi.nlm.nih.gov/38995904/ http://doi.org/10.1371/journal.pone.0306611 |
work_keys_str_mv |
AT leyriajimena octopamineisrequiredforsuccessfulreproductionintheclassicalinsectmodelrhodniusprolixus AT orchardian octopamineisrequiredforsuccessfulreproductionintheclassicalinsectmodelrhodniusprolixus AT langeangelab octopamineisrequiredforsuccessfulreproductionintheclassicalinsectmodelrhodniusprolixus |
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