Protective effect of nitro-oleic acids in neovascularization, gliosis and neurodegeneration in a mouse model of oxygen-induced retinopathy

2023 (2024 update): 5.0

Detalles Bibliográficos
Autores principales: Vaglienti, María Victoria, Subirada, Paula Virginia, Paz, María Constanza, Bonacci, Gustavo, Sanchez, Maria Cecilia
Otros Autores: https://orcid.org/0009-0007-0735-5356
Formato: publishedVersion article
Lenguaje:Inglés
Publicado: Association for Research in Vision and Ophthalmology. 2024
Acceso en línea:http://hdl.handle.net/11086/552548
https://iovs.arvojournals.org/article.aspx?articleid=2796328
Aporte de:
Repositorio Digital Universitario (UNC) de Universidad Nacional de Córdoba
id I10-R141-11086-552548
record_format dspace
institution Universidad Nacional de Córdoba
institution_str I-10
repository_str R-141
collection Repositorio Digital Universitario (UNC)
language Inglés
description 2023 (2024 update): 5.0
author2 https://orcid.org/0009-0007-0735-5356
author_facet https://orcid.org/0009-0007-0735-5356
Vaglienti, María Victoria
Subirada, Paula Virginia
Paz, María Constanza
Bonacci, Gustavo
Sanchez, Maria Cecilia
format publishedVersion
article
author Vaglienti, María Victoria
Subirada, Paula Virginia
Paz, María Constanza
Bonacci, Gustavo
Sanchez, Maria Cecilia
spellingShingle Vaglienti, María Victoria
Subirada, Paula Virginia
Paz, María Constanza
Bonacci, Gustavo
Sanchez, Maria Cecilia
Protective effect of nitro-oleic acids in neovascularization, gliosis and neurodegeneration in a mouse model of oxygen-induced retinopathy
author_sort Vaglienti, María Victoria
title Protective effect of nitro-oleic acids in neovascularization, gliosis and neurodegeneration in a mouse model of oxygen-induced retinopathy
title_short Protective effect of nitro-oleic acids in neovascularization, gliosis and neurodegeneration in a mouse model of oxygen-induced retinopathy
title_full Protective effect of nitro-oleic acids in neovascularization, gliosis and neurodegeneration in a mouse model of oxygen-induced retinopathy
title_fullStr Protective effect of nitro-oleic acids in neovascularization, gliosis and neurodegeneration in a mouse model of oxygen-induced retinopathy
title_full_unstemmed Protective effect of nitro-oleic acids in neovascularization, gliosis and neurodegeneration in a mouse model of oxygen-induced retinopathy
title_sort protective effect of nitro-oleic acids in neovascularization, gliosis and neurodegeneration in a mouse model of oxygen-induced retinopathy
publisher Association for Research in Vision and Ophthalmology.
publishDate 2024
url http://hdl.handle.net/11086/552548
https://iovs.arvojournals.org/article.aspx?articleid=2796328
work_keys_str_mv AT vaglientimariavictoria protectiveeffectofnitrooleicacidsinneovascularizationgliosisandneurodegenerationinamousemodelofoxygeninducedretinopathy
AT subiradapaulavirginia protectiveeffectofnitrooleicacidsinneovascularizationgliosisandneurodegenerationinamousemodelofoxygeninducedretinopathy
AT pazmariaconstanza protectiveeffectofnitrooleicacidsinneovascularizationgliosisandneurodegenerationinamousemodelofoxygeninducedretinopathy
AT bonaccigustavo protectiveeffectofnitrooleicacidsinneovascularizationgliosisandneurodegenerationinamousemodelofoxygeninducedretinopathy
AT sanchezmariacecilia protectiveeffectofnitrooleicacidsinneovascularizationgliosisandneurodegenerationinamousemodelofoxygeninducedretinopathy
_version_ 1806949164966215680
spelling I10-R141-11086-5525482024-07-08T06:22:18Z Protective effect of nitro-oleic acids in neovascularization, gliosis and neurodegeneration in a mouse model of oxygen-induced retinopathy Vaglienti, María Victoria Subirada, Paula Virginia Paz, María Constanza Bonacci, Gustavo Sanchez, Maria Cecilia https://orcid.org/0009-0007-0735-5356 https://orcid.org/0000-0003-3185-1951 https://orcid.org/0000-0002-1835-6713 https://orcid.org/0000-0002-9830-1850 https://orcid.org/0000-0003-4760-8518 2023 (2024 update): 5.0 info:eu-repo/semantics/publishedVersion Fil: Vaglienti, María Victoria. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas, Argentina. Fil: Vaglienti, María Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigaciones en Bioquímica Clínica e Inmunología, Argentina. Fil: Subirada, Paula Virginia. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas, Argentina. Fil: Subirada, Paula Virginia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigaciones en Bioquímica Clínica e Inmunología, Argentina. Fil: Paz, María Constanza. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas, Argentina. Fil: Paz, María Constanza. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigaciones en Bioquímica Clínica e Inmunología, Argentina. Fil: Bonacci, Gustavo. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas, Argentina. Fil: Bonacci, Gustavo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigaciones en Bioquímica Clínica e Inmunología, Argentina. Fil: Sanchez, Maria Cecilia. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas, Argentina. Fil: Sanchez, Maria Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigaciones en Bioquímica Clínica e Inmunología, Argentina. Purpose : Inflammation, oxidative and nitrosative stress are involved in neovascular retinopathies (NR). Nitro-fatty acids are important electrophilic signaling mediators with anti-inflammatory, antioxidant and cytoprotective properties. Therefore, our aim was to evaluate the effect of nitro-oleic acid (NO2-OA) in the OIR mouse model. Methods : Briefly, C57BL/6 mice (n=35) were exposed to 75% O2 from postnatal day (P)7 to P12, after which they were brought to room air (RA) for additional 5 (P17) or 14 days (P26). Age-match mice maintained in RA (n=25) were used as controls. OIR mice were intraocular injected with 5μM of NO2-OA (n=22) or vehicle (n=13) at P12 and intraperitoneal at P14, P17, P20, P23 with 15mg/Kg of NO2-OA or vehicle. At P17 or P26 mice were sacrificed. Some eyes were fixed for whole mount staining of retina and microscopic analysis and other retinas were used for WB or RT-PCR assays. Retinal functionality was assessed at P17 or P26 by scotopic ERG in dark-adapted mice. Some eyes were fixed to obtain cryosections for TUNEL assay. GraphPad Prism8 was used for statistical analysis. Results : NO2-OA induced vascular regrowth (p<0.05) and reduced neovascularization (p<0.001) at P17 OIR. RT-PCR revealed a significant increase in VEGF levels in OIR mice respect to RA mice (p<0.0001), but not difference was found between NO2-OA treatment and vehicle (p=0.9978). Regarding PEDF, neither significant changes were observed in OIR respect to RA mice (p=0.1394) nor between NO2-OA and vehicle (p=0.1849). In addition, WB of neural retinas showed that NO2-OA prevented glial stress (p<0.0001) induced in OIR at P17 (p=0.3160). NO2-OA did not modify the decrease (p=0.0177) in GS at P17 (p=0.0003). The ERG analysis showed a significant decrease in b-wave amplitude in OIR compared to RA mice. Also, a- and b- wave latencies were significantly increased in OIR respect to RA. Besides, WB analysis of retinas from RA and OIR mice revealed decreased levels of caspase-3 protein at P26 OIR. Finally, quantitative analysis showed that TUNEL-positive cells were significantly higher in P26 OIR compared to RA (p<0.05). All of these pathological events were prevented by NO2-OA (p=0.1362) (p=0.0004) (p<0.05). Conclusions : In conclusion, our study underscores the role of NO2-OA as potential treatment in the pathogenesis of NR in order to attenuate vascular and non-vascular alterations. This abstract was presented at the 2024 ARVO Annual Meeting, held in Seattle, WA, May 5-9, 2024. info:eu-repo/semantics/publishedVersion Fil: Vaglienti, María Victoria. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas, Argentina. Fil: Vaglienti, María Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigaciones en Bioquímica Clínica e Inmunología, Argentina. Fil: Subirada, Paula Virginia. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas, Argentina. Fil: Subirada, Paula Virginia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigaciones en Bioquímica Clínica e Inmunología, Argentina. Fil: Paz, María Constanza. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas, Argentina. Fil: Paz, María Constanza. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigaciones en Bioquímica Clínica e Inmunología, Argentina. Fil: Bonacci, Gustavo. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas, Argentina. Fil: Bonacci, Gustavo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigaciones en Bioquímica Clínica e Inmunología, Argentina. Fil: Sanchez, Maria Cecilia. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas, Argentina. Fil: Sanchez, Maria Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigaciones en Bioquímica Clínica e Inmunología, Argentina. 2024-07-04T12:18:56Z 2024-07-04T12:18:56Z 2024-06 article Vaglienti, M. V., Subirada, P. V., Paz, M. C., Bonacci, G., & Sanchez, M. C. (2024). Protective effect of nitro-oleic acids in neovascularization, gliosis and neurodegeneration in a mouse model of oxygen-induced retinopathy. Investigative Ophthalmology & Visual Science, 65(7), 270-270. http://hdl.handle.net/11086/552548 1552-5783 https://iovs.arvojournals.org/article.aspx?articleid=2796328 eng Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/ Association for Research in Vision and Ophthalmology.

Ejemplares similares