Sex differences in active DNA demethylation machinery during the critical period of brain masculinization

Fil: Bigarani, Rocío. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigación Médica Mercedes y Martín Ferreyra; Argentina.

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Detalles Bibliográficos
Autores principales: Bigarani, Rocío, Villarreal, Macarena, Cambiasso, María Julia, Cisternas, Carla Daniela
Formato: conferenceObject
Lenguaje:Inglés
Publicado: 2023
Materias:
DNA demethylation
sexual differentiation
epigenetics
Acceso en línea:http://hdl.handle.net/11086/549574
Aporte de:
Repositorio Digital Universitario (UNC) de Universidad Nacional de Córdoba
id I10-R141-11086-549574
record_format dspace
institution Universidad Nacional de Córdoba
institution_str I-10
repository_str R-141
collection Repositorio Digital Universitario (UNC)
language Inglés
topic DNA demethylation
sexual differentiation
epigenetics
spellingShingle DNA demethylation
sexual differentiation
epigenetics
Bigarani, Rocío
Villarreal, Macarena
Cambiasso, María Julia
Cisternas, Carla Daniela
Sex differences in active DNA demethylation machinery during the critical period of brain masculinization
topic_facet DNA demethylation
sexual differentiation
epigenetics
description Fil: Bigarani, Rocío. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigación Médica Mercedes y Martín Ferreyra; Argentina.
format conferenceObject
author Bigarani, Rocío
Villarreal, Macarena
Cambiasso, María Julia
Cisternas, Carla Daniela
author_facet Bigarani, Rocío
Villarreal, Macarena
Cambiasso, María Julia
Cisternas, Carla Daniela
author_sort Bigarani, Rocío
title Sex differences in active DNA demethylation machinery during the critical period of brain masculinization
title_short Sex differences in active DNA demethylation machinery during the critical period of brain masculinization
title_full Sex differences in active DNA demethylation machinery during the critical period of brain masculinization
title_fullStr Sex differences in active DNA demethylation machinery during the critical period of brain masculinization
title_full_unstemmed Sex differences in active DNA demethylation machinery during the critical period of brain masculinization
title_sort sex differences in active dna demethylation machinery during the critical period of brain masculinization
publishDate 2023
url http://hdl.handle.net/11086/549574
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AT villarrealmacarena sexdifferencesinactivednademethylationmachineryduringthecriticalperiodofbrainmasculinization
AT cambiassomariajulia sexdifferencesinactivednademethylationmachineryduringthecriticalperiodofbrainmasculinization
AT cisternascarladaniela sexdifferencesinactivednademethylationmachineryduringthecriticalperiodofbrainmasculinization
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spelling I10-R141-11086-5495742023-10-21T06:59:21Z Sex differences in active DNA demethylation machinery during the critical period of brain masculinization Bigarani, Rocío Villarreal, Macarena Cambiasso, María Julia Cisternas, Carla Daniela DNA demethylation sexual differentiation epigenetics Fil: Bigarani, Rocío. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigación Médica Mercedes y Martín Ferreyra; Argentina. Fil: Villarreal, Macarena. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigación Médica Mercedes y Martín Ferreyra; Argentina. Fil: Cambiasso, María Julia. Universidad Nacional de Córdoba. Facultad de Odontología. Cátedra de Biología Celular B; Argentina. Fil: Cambiasso, María Julia. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigación Médica Mercedes y Martín Ferreyra; Argentina Fil: Cisternas, Carla Daniela. Universidad Nacional de Córdoba. Facultad de Odontología. Cátedra de Biología Celular; Argentina. Fil: Cisternas, Carla Daniela. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Cátedra de Fisiología Animal; Argentina. Fil: Cisternas, Carla Daniela. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigación Médica Mercedes y Martín Ferreyra; Argentina. In mammals, perinatal peaks in gonadal testosterone organize a sex-typical neural circuitry during sensitive periods of development and a growing body of evidence suggest that epigenetic mechanisms are implicated. Some of the hormonal effects determine stable, sex-specific patterns of gene expression in neurons leading to the differentiation of neurochemical phenotypes relevant for the display of complex social behaviors in adulthood. We recently found that a neonatal inhibition of DNA methylation or demethylation reduces or eliminates sex differences in neurochemical phenotypes found in hypothalamic regions of the mouse brain. Here, we evaluated gene expression of TET 1-2-3, GAD45a-b and TDG (involved in the removal and replacement of 5-methylcytosine and 5-hydroxymethylcytosine) and the mRNA expression of the oxytocin receptor (OTR). mRNA expression was evaluated by qPCR in brain punches of prefrontal cortex (PFC) and preoptic area (POA) at postnatal day (P) 7 and P20. In PFC, we found sex differences (males > females) in TET3, TDG and Gad45b expression (p<0.05) and a trend for higher OTR-expression in males (p=0.06) at P7 suggesting higher DNA demethylation during the critical period of sexual differentiation. No sex differences were found at P20. Other brain regions and oxytocin expression are being evaluated. Overall, these results suggest that a sex-specific pattern of active DNA demethylation machinery could underline the organizational effects of hormones. https://san2022.saneurociencias.org.ar Fil: Bigarani, Rocío. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigación Médica Mercedes y Martín Ferreyra; Argentina. Fil: Villarreal, Macarena. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigación Médica Mercedes y Martín Ferreyra; Argentina. Fil: Cambiasso, María Julia. Universidad Nacional de Córdoba. Facultad de Odontología. Cátedra de Biología Celular B; Argentina. Fil: Cambiasso, María Julia. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigación Médica Mercedes y Martín Ferreyra; Argentina Fil: Cisternas, Carla Daniela. Universidad Nacional de Córdoba. Facultad de Odontología. Cátedra de Biología Celular; Argentina. Fil: Cisternas, Carla Daniela. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Cátedra de Fisiología Animal; Argentina. Fil: Cisternas, Carla Daniela. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigación Médica Mercedes y Martín Ferreyra; Argentina. Bioquímica y Biología Molecular (ídem 3.1.10) 2023-10-20T15:16:23Z 2023-10-20T15:16:23Z 2022 conferenceObject http://hdl.handle.net/11086/549574 eng Attribution-NonCommercial-ShareAlike 4.0 International http://creativecommons.org/licenses/by-nc-sa/4.0/ Electrónico y/o Digital

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