An in Vitro Biocompatibility Study of Conventional and Resin-modified Glass Ionomer Cements
Purpose: To evaluate the biocompatibility of a glass-ionomer (GIC) and a resin-modified glass-ionomer cement (RM-GIC), cell viability was examined in a model of human gingival fibroblasts using morphological, biochemical, and ionic patterns by means of phase contrast microscopy, lactate dehydrogenas...
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Formato: | article |
Lenguaje: | Inglés |
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2019
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Acceso en línea: | http://dx.doi.org/10.3290/j.jad.a29588 http://hdl.handle.net/11086/13456 |
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I10-R141-11086-13456 |
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institution |
Universidad Nacional de Córdoba |
institution_str |
I-10 |
repository_str |
R-141 |
collection |
Repositorio Digital Universitario (UNC) |
language |
Inglés |
topic |
GLASS-IONOMER CEMENTS; BIOCOMPATIBILITY; INTRACELLULAR; ELECTRON PROBE MICROANALYSIS |
spellingShingle |
GLASS-IONOMER CEMENTS; BIOCOMPATIBILITY; INTRACELLULAR; ELECTRON PROBE MICROANALYSIS Rodríguez, IA; Rozas, CA.; Campos-Sánchez, F.; Alaminos Mignorance, M.; Uribe Echevarria, J.; Campos A. An in Vitro Biocompatibility Study of Conventional and Resin-modified Glass Ionomer Cements |
topic_facet |
GLASS-IONOMER CEMENTS; BIOCOMPATIBILITY; INTRACELLULAR; ELECTRON PROBE MICROANALYSIS |
description |
Purpose: To evaluate the biocompatibility of a glass-ionomer (GIC) and a resin-modified glass-ionomer cement (RM-GIC), cell viability was examined in a model of human gingival fibroblasts using morphological, biochemical, and ionic patterns by means of phase contrast microscopy, lactate dehydrogenase (LDH) release, and quantitative x-ray microanalysis (EPXMA). Materials and Methods: The GIC Ketac-Molar Easymix (3M ESPE) and the RM-GIC Vitrebond (3M ESPE) were compared in human gingival fibroblasts exposed to the cements for 72 h. As controls, fibroblasts cultured with DMEM culture medium (negative control) and with 1% triton × (positive control) were used. Results: Light microscopic findings showed greater morphological alterations in cells exposed to RM-GIC than to GIC. The relative percentage of LDH released from the cells to the supernatant was significantly higher in RMGIC cultures than in the control. Quantitative x-ray microanalysis showed that cultures exposed to RM-GIC were characterized by an increase in intracellular Na and a decrease in intracellular Cl and K. These changes in ion composition were significant compared to control and GIC cultures. Conclusion: The three indicators of cellular biocompatibility after 72 h of exposure showed that RM-GIC led to more marked alterations than GIC in human gingival fibroblasts. |
format |
article |
author |
Rodríguez, IA; Rozas, CA.; Campos-Sánchez, F.; Alaminos Mignorance, M.; Uribe Echevarria, J.; Campos A. |
author_facet |
Rodríguez, IA; Rozas, CA.; Campos-Sánchez, F.; Alaminos Mignorance, M.; Uribe Echevarria, J.; Campos A. |
author_sort |
Rodríguez, IA; Rozas, CA.; Campos-Sánchez, F.; Alaminos Mignorance, M.; Uribe Echevarria, J.; Campos A. |
title |
An in Vitro Biocompatibility Study of Conventional and Resin-modified Glass Ionomer Cements |
title_short |
An in Vitro Biocompatibility Study of Conventional and Resin-modified Glass Ionomer Cements |
title_full |
An in Vitro Biocompatibility Study of Conventional and Resin-modified Glass Ionomer Cements |
title_fullStr |
An in Vitro Biocompatibility Study of Conventional and Resin-modified Glass Ionomer Cements |
title_full_unstemmed |
An in Vitro Biocompatibility Study of Conventional and Resin-modified Glass Ionomer Cements |
title_sort |
in vitro biocompatibility study of conventional and resin-modified glass ionomer cements |
publishDate |
2019 |
url |
http://dx.doi.org/10.3290/j.jad.a29588 http://hdl.handle.net/11086/13456 |
work_keys_str_mv |
AT rodrigueziarozascacampossanchezfalaminosmignorancemuribeechevarriajcamposa aninvitrobiocompatibilitystudyofconventionalandresinmodifiedglassionomercements AT rodrigueziarozascacampossanchezfalaminosmignorancemuribeechevarriajcamposa invitrobiocompatibilitystudyofconventionalandresinmodifiedglassionomercements |
bdutipo_str |
Repositorios |
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1764820391113523206 |