Molecular taxonomic analysis of individuals of the genus Lathyrus found in the northern extreme of Chile, using DNA barcoding
Background and aims: The present investigation corresponds to a molecular taxonomic analysis of floral samples of plants naturally established in a remote area of the Atacama Desert, Arica-Parinacota Region, Chile. In the first instance, the samples were morphologically attributed to the genus Lathy...
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Sociedad Argentina de Botánica
2020
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| Acceso en línea: | https://revistas.unc.edu.ar/index.php/BSAB/article/view/28030 |
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I10-R10-article-280302020-11-10T22:43:20Z Molecular taxonomic analysis of individuals of the genus Lathyrus found in the northern extreme of Chile, using DNA barcoding Análisis taxonómico molecular de individuos del genero Lathyrus hallados en el extremo norte de Chile, mediante DNA barcoding Arias Aburto, Mariana Ramírez Medina, Delia Contreras Diaz, Roberto César DNA barcoding ITS Lathyrus odoratus matK psbA-trnH rpoC1 DNA barcoding ITS Lathyrus odoratus matK psbA-trnH rpoC1 Background and aims: The present investigation corresponds to a molecular taxonomic analysis of floral samples of plants naturally established in a remote area of the Atacama Desert, Arica-Parinacota Region, Chile. In the first instance, the samples were morphologically attributed to the genus Lathyrus, however, according to current literature, no individuals of this genus have been registered in the region, given this, confusion has been generated in the identification of the collected samples. Therefore, the present research aims to genetically identify the presumably Lathyrus species, using DNA Barcoding. M&M: In the present study we evaluated these individuals by a phylogenetic analysis to determine their taxonomic identity from ITS (nuclear) sequence and psbA, matK and rpoC1 (plastidic) sequences. Results: The results demonstrated that the individuals are related to the species Lathyrus odoratus. Conclusions: The four DNA barcoding loci allowed the samples to be genetically identified as L. odoratus, which is found growing naturally in Chile. Introducción y objetivos: La presente investigación corresponde a un análisis taxonómico molecular de muestras florales obtenidas de plantas establecidas naturalmente en una zona recóndita del Desierto de Atacama, Región de Arica-Parinacota, Chile. En primera instancia, las muestras morfológicamente se atribuyeron al género Lathyrus, sin embargo, conforme a la literatura actual, no se han registrado individuos de este género en la región, dado esto, se ha generado confusión en la identificación de las muestras colectadas. Por lo cual, la presente investigación tiene por objetivo identificar genéticamente las especies presuntamente de Lathyrus, mediante DNA Barcoding. M&M: En el presente estudio se evaluaron estos individuos mediante un análisis filogenético para determinar su identidad taxonómica a partir de secuencias ITS (nuclear) y secuencias psbA, matK y rpoC1 (plastídicas). Resultados: Los resultados demostraron que los individuos están relacionados a la especie Lathyrus odoratus. Conclusiones: Los cuatro loci de DNA barcoding, permitieron identificar genéticamente las muestras como L. odoratus, el cual se encuentra creciendo de manera natural en Chile. Sociedad Argentina de Botánica 2020-09-24 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf text/html https://revistas.unc.edu.ar/index.php/BSAB/article/view/28030 Boletín de la Sociedad Argentina de Botánica (Journal of the Argentine Botanical Society; Vol. 55 No. 3 (2020): Setember; 479-492 Boletín de la Sociedad Argentina de Botánica; Vol. 55 Núm. 3 (2020): Septiembre; 479-492 Boletín de la Sociedad Argentina de Botánica; v. 55 n. 3 (2020): Septiembre; 479-492 1851-2372 0373-580X 10.31055/1851.2372.v55.n3 spa https://revistas.unc.edu.ar/index.php/BSAB/article/view/28030/31087 https://revistas.unc.edu.ar/index.php/BSAB/article/view/28030/31088 Derechos de autor 2020 Mariana Arias Aburto, Roberto César Contreras Diaz, Delia Ramírez Medina https://creativecommons.org/licenses/by-nc-nd/4.0 |
| institution |
Universidad Nacional de Córdoba |
| institution_str |
I-10 |
| repository_str |
R-10 |
| container_title_str |
Revistas de la UNC |
| language |
Español |
| format |
Artículo revista |
| topic |
DNA barcoding ITS Lathyrus odoratus matK psbA-trnH rpoC1 DNA barcoding ITS Lathyrus odoratus matK psbA-trnH rpoC1 |
| spellingShingle |
DNA barcoding ITS Lathyrus odoratus matK psbA-trnH rpoC1 DNA barcoding ITS Lathyrus odoratus matK psbA-trnH rpoC1 Arias Aburto, Mariana Ramírez Medina, Delia Contreras Diaz, Roberto César Molecular taxonomic analysis of individuals of the genus Lathyrus found in the northern extreme of Chile, using DNA barcoding |
| topic_facet |
DNA barcoding ITS Lathyrus odoratus matK psbA-trnH rpoC1 DNA barcoding ITS Lathyrus odoratus matK psbA-trnH rpoC1 |
| author |
Arias Aburto, Mariana Ramírez Medina, Delia Contreras Diaz, Roberto César |
| author_facet |
Arias Aburto, Mariana Ramírez Medina, Delia Contreras Diaz, Roberto César |
| author_sort |
Arias Aburto, Mariana |
| title |
Molecular taxonomic analysis of individuals of the genus Lathyrus found in the northern extreme of Chile, using DNA barcoding |
| title_short |
Molecular taxonomic analysis of individuals of the genus Lathyrus found in the northern extreme of Chile, using DNA barcoding |
| title_full |
Molecular taxonomic analysis of individuals of the genus Lathyrus found in the northern extreme of Chile, using DNA barcoding |
| title_fullStr |
Molecular taxonomic analysis of individuals of the genus Lathyrus found in the northern extreme of Chile, using DNA barcoding |
| title_full_unstemmed |
Molecular taxonomic analysis of individuals of the genus Lathyrus found in the northern extreme of Chile, using DNA barcoding |
| title_sort |
molecular taxonomic analysis of individuals of the genus lathyrus found in the northern extreme of chile, using dna barcoding |
| description |
Background and aims: The present investigation corresponds to a molecular taxonomic analysis of floral samples of plants naturally established in a remote area of the Atacama Desert, Arica-Parinacota Region, Chile. In the first instance, the samples were morphologically attributed to the genus Lathyrus, however, according to current literature, no individuals of this genus have been registered in the region, given this, confusion has been generated in the identification of the collected samples. Therefore, the present research aims to genetically identify the presumably Lathyrus species, using DNA Barcoding.
M&M: In the present study we evaluated these individuals by a phylogenetic analysis to determine their taxonomic identity from ITS (nuclear) sequence and psbA, matK and rpoC1 (plastidic) sequences.
Results: The results demonstrated that the individuals are related to the species Lathyrus odoratus.
Conclusions: The four DNA barcoding loci allowed the samples to be genetically identified as L. odoratus, which is found growing naturally in Chile. |
| publisher |
Sociedad Argentina de Botánica |
| publishDate |
2020 |
| url |
https://revistas.unc.edu.ar/index.php/BSAB/article/view/28030 |
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2022-08-20T01:21:21Z |
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