Interferon Gamma production by pas-positive t lymphocytes of chagasic patients infiltrated into human hearts in the sites of lesions
In the blood of chagasic patients, a high number of T-lymphocytes producers of a P.A.S.- positive substance was found, more numerous in chagasics with abnormal electrocardiogram. Further, we found such lymphocytes infiltrated in the chagasic heart. Here, we considered our hypothesis that those lymph...
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Universidad Nacional Cba. Facultad de Ciencias Médicas. Secretaria de Ciencia y Tecnología
2019
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| Acceso en línea: | https://revistas.unc.edu.ar/index.php/med/article/view/26472 |
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I10-R10-article-26472 |
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ojs |
| institution |
Universidad Nacional de Córdoba |
| institution_str |
I-10 |
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R-10 |
| container_title_str |
Revistas de la UNC |
| format |
Artículo revista |
| topic |
P.A.S.- positive T lymphocytes macrophages autoimmunity chagasis heart disease Interferon gamma Chagas pathogeny Linfocitos T P.A.S positivos macrófagos autoinmunidad enfermedad cardiaca chagásica Interferon gamma Chagas-patogenia |
| spellingShingle |
P.A.S.- positive T lymphocytes macrophages autoimmunity chagasis heart disease Interferon gamma Chagas pathogeny Linfocitos T P.A.S positivos macrófagos autoinmunidad enfermedad cardiaca chagásica Interferon gamma Chagas-patogenia Cabral, Humberto RA Novak, Ivon TC Glocker, T Mónica Castro Viera, Guido Interferon Gamma production by pas-positive t lymphocytes of chagasic patients infiltrated into human hearts in the sites of lesions |
| topic_facet |
P.A.S.- positive T lymphocytes macrophages autoimmunity chagasis heart disease Interferon gamma Chagas pathogeny Linfocitos T P.A.S positivos macrófagos autoinmunidad enfermedad cardiaca chagásica Interferon gamma Chagas-patogenia |
| author |
Cabral, Humberto RA Novak, Ivon TC Glocker, T Mónica Castro Viera, Guido |
| author_facet |
Cabral, Humberto RA Novak, Ivon TC Glocker, T Mónica Castro Viera, Guido |
| author_sort |
Cabral, Humberto RA |
| title |
Interferon Gamma production by pas-positive t lymphocytes of chagasic patients infiltrated into human hearts in the sites of lesions |
| title_short |
Interferon Gamma production by pas-positive t lymphocytes of chagasic patients infiltrated into human hearts in the sites of lesions |
| title_full |
Interferon Gamma production by pas-positive t lymphocytes of chagasic patients infiltrated into human hearts in the sites of lesions |
| title_fullStr |
Interferon Gamma production by pas-positive t lymphocytes of chagasic patients infiltrated into human hearts in the sites of lesions |
| title_full_unstemmed |
Interferon Gamma production by pas-positive t lymphocytes of chagasic patients infiltrated into human hearts in the sites of lesions |
| title_sort |
interferon gamma production by pas-positive t lymphocytes of chagasic patients infiltrated into human hearts in the sites of lesions |
| description |
In the blood of chagasic patients, a high number of T-lymphocytes producers of a P.A.S.- positive substance was found, more numerous in chagasics with abnormal electrocardiogram. Further, we found such lymphocytes infiltrated in the chagasic heart. Here, we considered our hypothesis that those lymphocytes would be Interferon-gamma producer cells.
Material and Methods: Heart samples of 8 patients deceased due to chagasic heart disease (ChHD). Cuts of 5 microns were submitted to monoclonal antibodies for human Interferon-gamma: to CD45RO for activated T lymphocytes; and to the classical Periodic acid Schiff reaction (P.A.S), respectively. In blood smears from chagasic patients with ChHD, the reactivity for anti Interferon gamma and for P.A.S. reaction was compared, regarding the respective positive cell number. The Myocardium status was compared with clinical date.
Results: In hearts, 65-75% of infiltrated lymphocytes were positive for IFN-gamma; similar values were found, in seriated cuts, of P.A.S.- positive lymphocytes, as well as of CD45RO+. In blood, there were 41%+- 9 of P.A.S.-positive lymphocytes, similar to positive cells for IFN- gamma.
Conclusions: The data indicates that the P.A.S.- positive lymphocytes from chagasic patients are producers of IFN-gamma in blood, as well as when infiltrated into the cardiac tissues. Such fact explains also the great affluence of macrophages in cardiac tissues in ChHD. The data indicate a strong response of T helper 1 type in this severe advance stage of chagas´ diseases. Neither Typanosome cruzi parasites or intracellular forms were seen in the hearts. This favors the data showing autoimmune mechanisms in this process. We open a question: to which antigen/s respond in the chagasic hearts the lymphocytes producers of IFN-gamma?. |
| publisher |
Universidad Nacional Cba. Facultad de Ciencias Médicas. Secretaria de Ciencia y Tecnología |
| publishDate |
2019 |
| url |
https://revistas.unc.edu.ar/index.php/med/article/view/26472 |
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2022-08-20T01:27:08Z |
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I10-R10-article-264722020-02-28T20:59:25Z Interferon Gamma production by pas-positive t lymphocytes of chagasic patients infiltrated into human hearts in the sites of lesions Producción de Interferon Gamma por linfocitos pas-positivos infiltrados en corazones de pacientes chagásicos en los sitios de lesiones Cabral, Humberto RA Novak, Ivon TC Glocker, T Mónica Castro Viera, Guido P.A.S.- positive T lymphocytes macrophages autoimmunity chagasis heart disease Interferon gamma Chagas pathogeny Linfocitos T P.A.S positivos macrófagos autoinmunidad enfermedad cardiaca chagásica Interferon gamma Chagas-patogenia In the blood of chagasic patients, a high number of T-lymphocytes producers of a P.A.S.- positive substance was found, more numerous in chagasics with abnormal electrocardiogram. Further, we found such lymphocytes infiltrated in the chagasic heart. Here, we considered our hypothesis that those lymphocytes would be Interferon-gamma producer cells. Material and Methods: Heart samples of 8 patients deceased due to chagasic heart disease (ChHD). Cuts of 5 microns were submitted to monoclonal antibodies for human Interferon-gamma: to CD45RO for activated T lymphocytes; and to the classical Periodic acid Schiff reaction (P.A.S), respectively. In blood smears from chagasic patients with ChHD, the reactivity for anti Interferon gamma and for P.A.S. reaction was compared, regarding the respective positive cell number. The Myocardium status was compared with clinical date. Results: In hearts, 65-75% of infiltrated lymphocytes were positive for IFN-gamma; similar values were found, in seriated cuts, of P.A.S.- positive lymphocytes, as well as of CD45RO+. In blood, there were 41%+- 9 of P.A.S.-positive lymphocytes, similar to positive cells for IFN- gamma. Conclusions: The data indicates that the P.A.S.- positive lymphocytes from chagasic patients are producers of IFN-gamma in blood, as well as when infiltrated into the cardiac tissues. Such fact explains also the great affluence of macrophages in cardiac tissues in ChHD. The data indicate a strong response of T helper 1 type in this severe advance stage of chagas´ diseases. Neither Typanosome cruzi parasites or intracellular forms were seen in the hearts. This favors the data showing autoimmune mechanisms in this process. We open a question: to which antigen/s respond in the chagasic hearts the lymphocytes producers of IFN-gamma?. En sangre de chagásicos en trabajos previos se halló un número alto de linfocitos T productores de sustancia P.A.S.- positiva, más numerosos en chagásicos con electrocar- diograma anormal. Luego los hallamos infiltrados en el corazón chagásico. En este trabajo consideramos nuestra hipótesis de que esa substancia linfocitaria sería Interferon-gamma. Material y métodos. Muestras de corazones de 8 chagásicos fallecidos por muerte cardiaca. Se utilizaron anticuerpos monoclonares anti-IFN gamma humano; para linfocitos T activados (CD45 RO), y la reacción del P.A.S, respectivamente. Las células positivas contaron en 50 campos a 400x y el estado de miocardio se comparó con datos clínicos. También, en frotis de sangre de pacientes chagásicos con ECG anorma, investigamos inmunomarcación con anti interferon gamma y las P.A.S- positividad, respectivamente. Resultados El mayor número de células infiltrantes intracardiacas (65-75%) resultaron positivos para IFN gamma, y similares valores para P.A.S - positividad y para CD45 RO. En sangre hallamos 41%+-7 de linfocitos P.A.S.- positivos: y similar de positivos para IFN- gamma. Conclusiones: Los datos muestran los linfocitos P.A.S .- positivos de los chagásicos producen IFN- gamma tanto en sangre, como infiltrados en tejidos cardíacos, e identifican una fuerte respuesta T-helper 1. También explican la gran afluencia de macrófagos en dichos tejidos cardíacos. No se vieron parásitos T. cruzi ni formas intracelulares. Esos hechos refuerzan los datos que indican existencia de autoinmunidad en Chagas. Abrimos una pregunta: ¿ A qué antígeno/s responden en el corazón chagásico los linfocitos T P.A.S.- positivos productores de IFN - gamma? Universidad Nacional Cba. Facultad de Ciencias Médicas. Secretaria de Ciencia y Tecnología 2019-11-21 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion https://revistas.unc.edu.ar/index.php/med/article/view/26472 Revista de la Facultad de Ciencias Médicas de Córdoba.; Vol. 63 No. 3 (2006); 47 - 52 Revista de la Facultad de Ciencias Médicas de Córdoba; Vol. 63 Núm. 3 (2006); 47 - 52 Revista da Faculdade de Ciências Médicas de Córdoba; v. 63 n. 3 (2006); 47 - 52 1853-0605 0014-6722 Derechos de autor 2019 Universidad Nacional de Córdoba |