Development of a non-radioactive molecular hybridization probe for detecting Strawberry mottle virus in strawberry

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The vegetative propagation of strawberries favors transmission of systemic pathogens, such as viruses, which are one of the main yield-limiting factors for this crop. More than 20 viruses have been described as infecting this species; one of the most frequent is the Strawberry mottle virus (SMoV), w...

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Autores principales: Asinari, F., Cafrune, E.E., Guzman, F.A., Conci, L.R., Conci, V.C.
Formato: Artículo revista
Lenguaje:Inglés
Publicado: Facultad de Ciencias Agropecuarias. 2016
Materias:
Fragaria x ananassa
molecular techniques
screening viruses
virus diagnosis.
técnicas moleculares
muestreo de virus
diagnóstico de virus.
Acceso en línea:https://revistas.unc.edu.ar/index.php/agris/article/view/16570
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Revistas de la UNC de Universidad Nacional de Córdoba
id I10-R10-article-16570
record_format ojs
spelling I10-R10-article-165702019-03-11T19:30:48Z Development of a non-radioactive molecular hybridization probe for detecting Strawberry mottle virus in strawberry Desarrollo de una sonda de hibridación molecular no radioactiva para la detección de Strawberry mottle virus en frutilla Asinari, F. Cafrune, E.E. Guzman, F.A. Conci, L.R. Conci, V.C. Fragaria x ananassa molecular techniques screening viruses virus diagnosis. Fragaria x ananassa técnicas moleculares muestreo de virus diagnóstico de virus. The vegetative propagation of strawberries favors transmission of systemic pathogens, such as viruses, which are one of the main yield-limiting factors for this crop. More than 20 viruses have been described as infecting this species; one of the most frequent is the Strawberry mottle virus (SMoV), which is responsible for significant economic losses. SMoV is usually detected by reverse transcription polymerase chain reaction (RT-PCR), given that serum is not available for serological tests. In this study, a non-radioactive molecular probe was developed for SMoV detection. The cDNA was synthesized by RT-PCR using specific primers designed from the 3'UTR region of the viral genome. The cloned cDNA segment was labeled and used as a probe. Six RNA extraction protocols were evaluated, and the modified cetyltrimethylammonium bromide (CTAB) method showed the highest sensitivity level. Leaves at different phenological stages and petioles were evaluated; the highest reaction was observed in old leaves and in petioles. La propagación vegetativa del cultivo de frutilla favorece la transmisión de patógenos sistémicos, como es el caso de los virus, que constituyen uno de los principales factores limitantes. Se han descripto más de 20 virus que infectan esta especie; el Strawberry mottle virus (SMoV) es uno de los más frecuentes y responsable de importantes pérdidas económicas. Debido a la falta de antisuero disponible comercialmente para un diagnóstico serológico, el SMoV es detectado fundamentalmente mediante reacción en cadena de la polimerasa con transcripción reversa (RT-PCR). En este estudio se desarrolló una sonda de hibridación molecular no radioactiva para su detección. Se sintetizó cDNA con cebadores específicos diseñados a partir de la región 3' no codificante del genoma viral. El cDNA obtenido fue clonado, marcado y utilizado como sonda. Se evaluaron seis protocolos de extracción de ARN viral a partir de plantas infectadas, de los cuales el método de bromuro de cetiltrimetilamonio modificado (CTAB) fue el más eficiente. Se evaluaron hojas de diferentes estados fenológicos y pecíolos, y fueron las hojas viejas y los pecíolos los que mostraron mayor reacción. Facultad de Ciencias Agropecuarias. 2016-06-30 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf https://revistas.unc.edu.ar/index.php/agris/article/view/16570 AgriScientia; Vol. 33 No. 1 (2016); 39-45 AgriScientia; Vol. 33 Núm. 1 (2016); 39-45 1668-298X 10.31047/1668.298x.v33.n1 eng https://revistas.unc.edu.ar/index.php/agris/article/view/16570/16381
institution Universidad Nacional de Córdoba
institution_str I-10
repository_str R-10
container_title_str Revistas de la UNC
language Inglés
format Artículo revista
topic Fragaria x ananassa
molecular techniques
screening viruses
virus diagnosis.
Fragaria x ananassa
técnicas moleculares
muestreo de virus
diagnóstico de virus.
spellingShingle Fragaria x ananassa
molecular techniques
screening viruses
virus diagnosis.
Fragaria x ananassa
técnicas moleculares
muestreo de virus
diagnóstico de virus.
Asinari, F.
Cafrune, E.E.
Guzman, F.A.
Conci, L.R.
Conci, V.C.
Development of a non-radioactive molecular hybridization probe for detecting Strawberry mottle virus in strawberry
topic_facet Fragaria x ananassa
molecular techniques
screening viruses
virus diagnosis.
Fragaria x ananassa
técnicas moleculares
muestreo de virus
diagnóstico de virus.
author Asinari, F.
Cafrune, E.E.
Guzman, F.A.
Conci, L.R.
Conci, V.C.
author_facet Asinari, F.
Cafrune, E.E.
Guzman, F.A.
Conci, L.R.
Conci, V.C.
author_sort Asinari, F.
title Development of a non-radioactive molecular hybridization probe for detecting Strawberry mottle virus in strawberry
title_short Development of a non-radioactive molecular hybridization probe for detecting Strawberry mottle virus in strawberry
title_full Development of a non-radioactive molecular hybridization probe for detecting Strawberry mottle virus in strawberry
title_fullStr Development of a non-radioactive molecular hybridization probe for detecting Strawberry mottle virus in strawberry
title_full_unstemmed Development of a non-radioactive molecular hybridization probe for detecting Strawberry mottle virus in strawberry
title_sort development of a non-radioactive molecular hybridization probe for detecting strawberry mottle virus in strawberry
description The vegetative propagation of strawberries favors transmission of systemic pathogens, such as viruses, which are one of the main yield-limiting factors for this crop. More than 20 viruses have been described as infecting this species; one of the most frequent is the Strawberry mottle virus (SMoV), which is responsible for significant economic losses. SMoV is usually detected by reverse transcription polymerase chain reaction (RT-PCR), given that serum is not available for serological tests. In this study, a non-radioactive molecular probe was developed for SMoV detection. The cDNA was synthesized by RT-PCR using specific primers designed from the 3'UTR region of the viral genome. The cloned cDNA segment was labeled and used as a probe. Six RNA extraction protocols were evaluated, and the modified cetyltrimethylammonium bromide (CTAB) method showed the highest sensitivity level. Leaves at different phenological stages and petioles were evaluated; the highest reaction was observed in old leaves and in petioles.
publisher Facultad de Ciencias Agropecuarias.
publishDate 2016
url https://revistas.unc.edu.ar/index.php/agris/article/view/16570
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first_indexed 2022-08-20T01:01:32Z
last_indexed 2022-08-20T01:01:32Z
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