Baseline values of Micronuclei and Comet Assay in the lizard Tupinambis merianae (Teiidae, Squamata)
The Micronucleus test (MN) and Comet assay (CA) are currently the most widely used methods that allow the characterization of DNA damage induced by physical and chemical agents in wild species. The continuous expansion of the cultivated areas in Argentina, since the introduction of transgenic crops,...
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2012
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| LEADER | 15773caa a22015497a 4500 | ||
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| 005 | 20230518203912.0 | ||
| 008 | 190411s2012 xx ||||fo|||| 00| 0 eng|d | ||
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| 024 | 7 | |2 cas |a hydrogen peroxide, 7722-84-1; Hydrogen Peroxide, 7722-84-1; Oxidants | |
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| 100 | 1 | |a Schaumburg, L.G. | |
| 245 | 1 | 0 | |a Baseline values of Micronuclei and Comet Assay in the lizard Tupinambis merianae (Teiidae, Squamata) |
| 260 | |c 2012 | ||
| 270 | 1 | 0 | |m Schaumburg, L.G.; Laboratorio de Zoología Aplicada: Anexo Vertebrados, Facultad de Humanidades y Ciencias, UNL/Ministerio de Aguas, Servicios Públicos y Medio Ambiente, Aristóbulo del Valle 8700, CP: 3000, Santa Fe, Argentina; email: giseschaumburg@yahoo.com.ar |
| 506 | |2 openaire |e Política editorial | ||
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| 504 | |a Poletta, G.L., Larriera, A., Kleinsorge, E., Mudry, M.D., Genotoxicity of the herbicide formulation Roundup® (glyphosate) in broad-snouted caiman (Caiman latirostris) evidenced by the Comet assay and the Micronucleus test (2009) Mutat. Res., 672, pp. 95-102 | ||
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| 504 | |a Schaumburg, L.G., Poletta, G.L., Imhof, A., Siroski, P.A., Ultraviolet radiation-induced genotoxic effects in the broad-snouted caiman, Caiman latirostris (2010) Mutat. Res., 700, pp. 67-70 | ||
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| 520 | 3 | |a The Micronucleus test (MN) and Comet assay (CA) are currently the most widely used methods that allow the characterization of DNA damage induced by physical and chemical agents in wild species. The continuous expansion of the cultivated areas in Argentina, since the introduction of transgenic crops, mainly soy, in association with the increased use of pesticides, transformed deeply the natural environments where the lizard Tupinambis merianae (tegu lizard) occurs. Despite the fact that reptiles have shown to be excellent bioindicators of environmental contaminants, there is no record of genotoxicity studies in T. merianae. The aim of the present study was to adjust the MN test and CA protocols to be applied in erythrocytes of T. merianae, and determine the baseline values of DNA damage in this species. We used 20 adult lizards (10 males: 10 females) from Estación Zoológica Experimental "Granja La Esmeralda" (Santa Fe, Argentina). Peripheral blood samples were collected from all animals and the MN test and CA applied according to the protocols established for other reptilian species. We test critical parameters of CA protocol (cell density, unwinding and electrophoresis times) using increasing concentrations of H2O2 (10, 25 and 50μM) as a known genotoxic agent to induce DNA damage. Based on this, we determined the most suitable conditions for the CA in this species: a cell density of 4×103 erythrocytes per slide, 10min of unwinding and 15min of electrophoresis at 0.90V/cm approximately. The baseline frequency of micronuclei (BFMN=MN/1000 erythrocytes counted) determined for this species was 0.95±0.27 and the basal damage index (BDI: calculated from 100 comet images classified in arbitrary units)=103.85±0.97. No differences were observed between sexes in the BFMN or BDI (p>0.05), and no relation was found between baseline values and length or weight of the analyzed animals (p>0.05). These results demonstrated the sensitivity of both biomarkers of genotoxicity to be applied in erythrocytes of this species, with baseline values comparable to those reported in other reptilian species. These results allow us to propose the tegu lizard for future in vivo studies to assess the genotoxicity of different agents, including those possibly affecting it in its natural geographic distribution. © 2012 Elsevier Inc. |l eng | |
| 536 | |a Detalles de la financiación: Universidad de Buenos Aires, PIP 112 CONICET, MDM PIP 112 | ||
| 536 | |a Detalles de la financiación: This study was supported by Laboratorio de Zoología Aplicada: Anexo Vertebrados (Facultad de Humanidades y Ciencias, UNL/MASPyMA) , Universidad de Buenos Aires (MDM PIP 112 CONICET, Buenos Aires, Argentina) and Proyecto Iguana (Res. 031/07, Secretaria de Estado de Medio Ambiente y Desarrollo Sustentable de la Provincia de Santa Fe) . We would like to thank members of Proyecto Iguana and Proyecto Yacaré for their help in this study. This study is part of LGS research as a doctoral fellow of CONICET in work collaboration between the UNL/MASPyMA and UBA. | ||
| 593 | |a Grupo de Investigación en Biología Evolutiva (GIBE), IEGEBA (CONICET-UBA), Labs. 43-46, FCEyN, Piso 4, Pabellon II, Cdad. Univ, Intendente Guiraldes 2160, CP: 1428EGA, Buenos Aires, Argentina | ||
| 593 | |a Laboratorio de Zoología Aplicada: Anexo Vertebrados, Facultad de Humanidades y Ciencias, UNL/Ministerio de Aguas, Servicios Públicos y Medio Ambiente, Aristóbulo del Valle 8700, CP: 3000, Santa Fe, Argentina | ||
| 593 | |a Cátedra de Toxicología, Farmacología y Bioquímica Legal, Facultad de Bioquímica y Ciencias Biológicas, UNL, CC 242 Paraje El Pozo, CP: 3000, Santa Fe, Argentina | ||
| 593 | |a Dir. Gral. Manejo Sustentable Fauna y Flora, Aristóbulo del Valle 8700, CP: 3000, Santa Fe, Argentina | ||
| 593 | |a National Council of Scientific and Technical Research (CONICET), Argentina | ||
| 690 | 1 | 0 | |a GENOTOXICITY |
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| 690 | 1 | 0 | |a TUPINAMBIS MERIANAE |
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| 690 | 1 | 0 | |a ANIMALIA |
| 690 | 1 | 0 | |a REPTILIA |
| 690 | 1 | 0 | |a SQUAMATA |
| 690 | 1 | 0 | |a TEIIDAE |
| 690 | 1 | 0 | |a TUPINAMBIS MERIANAE |
| 690 | 1 | 0 | |a TUPINAMBIS NIGROPUNCTATUS |
| 650 | 1 | 7 | |2 spines |a REPTILES |
| 651 | 4 | |a ARGENTINA | |
| 651 | 4 | |a ARGENTINA | |
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| 700 | 1 | |a Poletta, G.L. | |
| 700 | 1 | |a Siroski, P.A. | |
| 700 | 1 | |a Mudry, M.D. | |
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