A rapid method for the screening of plasmids from Clostridium acetobutylicum and other Clostridium sp.
A rapid method was developed for plasmid DNA screening from micro volume cultures of Clostridium acetobutylicum. It involves protoplastization by lysozyme, nuclease inhibition by incorporating EDTA or DEP, followed by lysis with high concentration of SDS. The whole lysate is applied directly to elec...
Guardado en:
| Autor principal: | |
|---|---|
| Otros Autores: | , |
| Formato: | Capítulo de libro |
| Lenguaje: | Inglés |
| Publicado: |
Kluwer Academic Publishers
1987
|
| Acceso en línea: | Registro en Scopus DOI Handle Registro en la Biblioteca Digital |
| Aporte de: | Registro referencial: Solicitar el recurso aquí |
| Sumario: | A rapid method was developed for plasmid DNA screening from micro volume cultures of Clostridium acetobutylicum. It involves protoplastization by lysozyme, nuclease inhibition by incorporating EDTA or DEP, followed by lysis with high concentration of SDS. The whole lysate is applied directly to electrophoretic analysis. © 1987 Chapman & Hall. |
|---|---|
| Bibliografía: | Benassi, F.O., Bloos, R.K., de Rambaldo, L.A., (1983) Rev. Arg. de Microbiol., 15, pp. 65-71 Birnboim, H.C., Doly, J., (1979) Nucleic. Acids. Res., 7, pp. 1513-1523 Blaschek, H.P., Klacik, M.A., (1984) J.Bacteriol., 147, pp. 262-266 Davis, R.W., Botstein, D., Roth, J.R., (1980) advanced Bacterial Genetics Laboratory Manual, p. 201. , Cold Spring Harbor Laboratory, New York Eckhardt, T., (1978) Plasmid, 1, pp. 584-588 Floccari, M.E., Schijman, A.G., Méndez, B.S., (1986) Abstracts of the IV Southern Cone Meeting of the Panamerican Association of Biochemical Societies Nov26–29, , Pucon, Chile Guerry, P., Le Blanc, D.J., Falkow, S., (1973) J. Bacteriol., 116, pp. 1064-1066 Herrero, A.H., Gómez, R.F., (1980) Appl. Environ Microbiol., 40, pp. 571-577 Jones, D.T., Jones, W.A., Woods, D.R., (1985) J.Gen. Microibol., 131, pp. 1213-1216 Lin, Y.L., Blaschek, H.P., (1984) Appl. Environ. Microbiol., 48, pp. 737-742 Maniatis, T., Fritsch, E.F., Sambrook, J., (1982) Molecular cloning, p. 160. , Cold Spring Harbor Laboratory, Cold Spring Harbor. New York Meyers, J.A., Sánchez, D., Elwell, L.B., Falkow, S., (1976) J. Bacteriol., 127, pp. 1529-1537 O'Brien, R.W., Morris, J.A., (1971) J. Gen. Microbiol., 68, pp. 307-318 Oultram, J., Young, M., (1985) FEMS. Microbiol. Lett., 27, pp. 129-134 Reysset, G., Sebald, M., (1985) Ann. Inst. Pasteur Microbiol., 136, pp. 275-282 Schnaitman, C.A., (1971) J. Bacteriol., 108, pp. 553-563 Strom, M.S., Eklund, Poysky, F., (1984) Appl.Env.Microbiol., 48, pp. 956-963 Truffaut, N., Sebald, M., (1983) Mol.Gen. Genet., 189, pp. 178-180 Urano, N., Karube, I., Suzoki, S., Yamada, T., (1983) Eur. J. Appl. Microbiol. Biotechnol., 17, pp. 349-354 Youngman, P.J., Perkins, B.J., Losik, R., (1983) Proc. Natl. Acad.Sci. USA., 80, pp. 2305-2309 Yu, P., Pearce, L.E., (1986) Biotechnol. Lett., 8, pp. 469-474 |
| ISSN: | 0951208X |
| DOI: | 10.1007/BF00227554 |