A protein-resistant matrix for electrochemical based recognition assays

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In this work, two well known polymers, carboxymethyl dextran and poly(ethyleneglycol), are used for easily producing a platform for electrochemical affinity assays, avoiding nonspecific adsorption of proteins. In this way, modified electrode surfaces able to bind a recognition agent are constructed...

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Detalles Bibliográficos
Autor principal: Pallarola, D.
Otros Autores: Domenianni, L., Priano, Graciela Inés, Battaglini, Fernando
Formato: Capítulo de libro
Lenguaje:Inglés
Publicado: 2007
Acceso en línea:Registro en Scopus
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Biblioteca Central Dr. Luis F. Leloir (FCEN) de Universidad de Buenos Aires
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100 1 |a Pallarola, D. 
245 1 2 |a A protein-resistant matrix for electrochemical based recognition assays 
260 |c 2007 
270 1 0 |m Pallarola, D.; INQUIMAE, Departamento de Química Inorgánica, Analítica Y Química Física, Pabellón 2, C1428EHA Buenos Aires, Argentina 
504 |a Senaratne, W., Andruzzi, L., Ober, C.K., (2005) Biomacromol, 6, p. 2427 
504 |a Bi, H., Zhong, W., Meng, S., Kong, J., Yang, P., Liu, B., (2006) Anal. Chem, 78, p. 3399 
504 |a Wang, A.J., Xu, J.J., Chen, H.Y., (2006) Anal. Chim. Acta, 569, p. 188 
504 |a Shin, D., Tryk, D.A., Fujishima, A., Merkoçi, A., Wang, J., (2004) Electroanalysis, 17, p. 305 
504 |a Liu, J., Sun, X., Lee, M.L., (2005) Anal. Chem, 77, p. 6280 
504 |a Uchida, K., Otsuka, H., Kaneko, M., Kataoka, K., Nagasaki, Y., (2005) Anal. Chem, 77, p. 1075 
504 |a Masson, J.-F., Battaglia, T.M., Davidson, M.J., Kim, Y.-C., Prakash, A.M.C., Beaudoin, S., Booksh, K.S., (2005) Talanta, 67, p. 918 
504 |a Österberg, E., Bergström, K., Holmberg, K., Riggs, J.A., Van Alstine, J.M., Schuman, T.P., Burns, N.L., Harris, J.M., (1993) Colloids Surf. A: Physicochem. Eng. Aspects, 77, p. 159 
504 |a De Sousa Delgado, A., Léonard, M., Dellacherie, E., (2001) Langmuir, 17, p. 4386 
504 |a Löfas, S., Johnsson, B., (1990) J. Chem. Soc. Chem. Commun, 21, p. 1526 
504 |a Johnsson, B., Löfas, S., Lindqvist, G., (1991) Anal. Biochem, 198, p. 268 
504 |a Limoges, B., Savéant, J.M., Yazidi, D., (2003) J. Am. Chem. Soc, 125, p. 9192 
504 |a Rogan, G.J., Dudin, Y.A., Lee, T.C., Magin, K.M., Astwood, J.D., Bhakta, N.S., Leach, J.N., Fuchs, R.L., (1999) Food Control, 10, p. 407 
504 |a Robinson, S.A., Rosenzweig, S.A., (2004) Biochem, 43, p. 11533 
504 |a Kober, E.M., Caspar, J.V., Sullivan, B.P., Meyer, T.J., (1988) Inorg. Chem, 27, p. 4587 
504 |a Hattori, M., Nagasawa, K., Ametani, A., Kaminogawa, S., Takahashit, K., (1994) J. Agric. Food Chem, 42, p. 2120 
504 |a Kobayasi, K., Yoshida, T., Takahashi, K., Hattori, M., (2003) Biocon jugate Chem, 14, p. 168 
504 |a Dequaire, M., Limoges, B., Moiroux, J., Savéant, J.M., (2002) J. Am. Chem. Soc, 124, p. 240 
504 |a Böhmer, M.R., Koopal, L.K., Janssen, R., Lee, E.M., Thomas, R.K., Rennie, A.R., (1992) Langmuir, 8, p. 2228 
504 |a Tiberg, F.J., (1996) J. Chem. Soc. Faraday Trans, 92, p. 531 
504 |a Forzani, E.S., Otero, M., Pérez, M.A., López Teijelo, M., Calvo, E.J., (2002) Langmuir, 18, p. 4020 
504 |a De Feijter, J.A., Benjamins, J., Veer, F.A., (1978) Biopolymers, 17, p. 1759 
504 |a Piehler, J., Brecht, A., Hehl, K., Gauglitz, G., (1999) Colloids Surf. B: Biointerf, 13, p. 325 
504 |a Cullen, D.C., Brown, R.G.W., Lowe, C.R., (1987) Biosensors, 3, p. 211 
504 |a Lu, H.C., Chen, H.M., Lin, Y.S., Lin, J.W., (2000) Biotechnol. Prog, 16, p. 116 
504 |a Ostuni, E., Chapman, R.G., Liang, M.N., Meluleni, G., Pier, G., Ingber, D.E., Whitesides, G.M., (2001) Langmuir, 17, p. 6336 
504 |a Bergstrom, J., Löfas, S., Johnsson, B., (1993), USP 5242828; Walczak, M.M., Popenoe, D.D., Deinhammer, R.S., Lamp, B.D., Chung, C., Porter, M.D., (1991) Langmuir, 7, p. 2687 
504 |a Walczac, M.M., Aves, C.A., Lamp, B.D., Porter, M.D., (1995) J. Electroanal. Chem, 396, p. 103 
504 |a El-Deab, M., Ohsaza, T., (2004) Electrochim. Acta, 49, p. 2189 
504 |a Raiber, K., Terfort, A., Benndorf, C., Krings, N., Strehblow, H.H., (2005) Surf. Sci, 595, p. 56  |a Chapman, R.G., Ostuni, E., Liang, M.N., Meluleni, G., Kim, E., Yan, L., Pier, G., Whitesides, G.M., (2001) Langmuir, 17, p. 1225 
504 |a Osterberg, E., Bergstrom, K., Holmberg, K., Schuman, T.P., Riggs, J.A., Burns, N.L., Van Alstine, J.M., Harris, J.M., (1995) J. Biomed. Mater. Res, 29, p. 741. , and references therein 
504 |a Priano, G., Battaglini, F., (2005) Anal. Chem, 77, p. 4976 
504 |a Bartlett, P.N., Tebbutt, B.L.P., Tyrrell, C.H., (1992) Anal. Chem, 64, p. 138 
506 |2 openaire  |e Política editorial 
520 3 |a In this work, two well known polymers, carboxymethyl dextran and poly(ethyleneglycol), are used for easily producing a platform for electrochemical affinity assays, avoiding nonspecific adsorption of proteins. In this way, modified electrode surfaces able to bind a recognition agent are constructed through simple and reliable reactions. 24 and 70 KDa Carboxymethyl Dextrans (CMDex24 and CMDex70) were covalently attached to gold through 2-aminoethylthiol, allowing the fast electron transfer reaction of a redox mediator. Based on ellipsometry and amperometry results, the nonspecific adsorption of neutravidin-horseradish peroxidase (NHRP), a conjugate used as indicating molecule, was studied considering the physicochemical characteristics of the CMDex films. The experimental results show that CMDex70 produces a dense structure reducing drastically the nonspecific adsorption of NHRP, still allowing the access of the redox mediator. Poly(ethylene glycol) derivates, combined with CMDex, were also investigated. Methoxypolyethylene glycol succinate N-hydroxysuccinimide ester (MW: 5000 Da), combined with either CMDex 24 or 70, was found also to reduce the nonspecific interactions. Additionally, the specific binding of NHRP, and the effect of the film structure in the amperometric response, were established using electrodes modified covalently with the specific biotin counterpart. © 2007 Wiley-VCH Verlag GmbH & Co. KGaA.  |l eng 
593 |a INQUIMAE, Departamento de Química Inorgánica, Analítica Y Química Física, Pabellón 2, C1428EHA Buenos Aires, Argentina 
690 1 0 |a AMPEROMETRY 
690 1 0 |a DEXTRAN DERIVATIVES 
690 1 0 |a NEUTRAVIDIN 
690 1 0 |a NONSPECIFIC ADSORPTION 
690 1 0 |a PEROXIDASE 
690 1 0 |a PROTEINS 
700 1 |a Domenianni, L. 
700 1 |a Priano, Graciela Inés 
700 1 |a Battaglini, Fernando 
773 0 |d 2007  |g v. 19  |h pp. 690-697  |k n. 6  |p Electroanalysis  |x 10400397  |t Electroanalysis 
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856 4 0 |u https://doi.org/10.1002/elan.200603801  |y DOI 
856 4 0 |u https://hdl.handle.net/20.500.12110/paper_10400397_v19_n6_p690_Pallarola  |y Handle 
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962 |a info:eu-repo/semantics/article  |a info:ar-repo/semantics/artículo  |b info:eu-repo/semantics/publishedVersion 
999 |c 67650 

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