Evidence of an association between poly(3-hydroxybutyrate) accumulation and phosphotransbutyrylase expression in Bacillus megaterium

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Molecular analysis of a genomic region of Bacillus megaterium, a polyhydroxybutyrate (PHB)-producing microorganism, revealed the presence of a gene coding for the enzyme phosphotransbutyrylase (Ptb). Enzyme activity was measured throughout the different growth phases of B. megaterium and was found t...

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Detalles Bibliográficos
Autor principal: Vazquez, G.J
Otros Autores: Pettinari, M.J, Méndez, B.S
Formato: Capítulo de libro
Lenguaje:Inglés
Publicado: Springer-Verlag GmbH Co. KG 2003
Acceso en línea:Registro en Scopus
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Aporte de:Registro referencial: Solicitar el recurso aquí
Biblioteca Central Dr. Luis F. Leloir (FCEN) de Universidad de Buenos Aires
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024 7 |2 scopus  |a 2-s2.0-0141992983 
024 7 |2 cas  |a glucose, 50-99-7, 84778-64-3; isoleucine, 7004-09-3, 73-32-5; poly(3 hydroxybutyric acid), 26063-00-3; valine, 7004-03-7, 72-18-4 
040 |a Scopus  |b spa  |c AR-BaUEN  |d AR-BaUEN 
100 1 |a Vazquez, G.J. 
245 1 0 |a Evidence of an association between poly(3-hydroxybutyrate) accumulation and phosphotransbutyrylase expression in Bacillus megaterium 
260 |b Springer-Verlag GmbH Co. KG  |c 2003 
270 1 0 |m Méndez, B.S.; Depto. de Quím. Biol., Universidad de Buenos Aires, Fac. de Ciencias Exactas y Naturales, Buenos Aires, Argentina; email: bea@qb.fcen.uba.ar 
506 |2 openaire  |e Política editorial 
504 |a Braunegg, G., Sonnleitner, R., Lafferty, R., A rapid gas Chomatographic method for the determination of poly-β-hydroxybutiric acid in microbial biomass (1978) Eur J Appl Microbiol Biotechnol, 6, pp. 29-37 
504 |a Cary, J.W., Petersen, D.J., Papoutsakis, E.T., Bennett, G.N., Cloning and expression of Clostridium acetobutylicum phosphotransbutyrylase and butyrate kinase genes in Escherichia coli (1988) J Bacteriol, 170, pp. 4613-4618 
504 |a Debarbouille, M., Gardan, R., Arnaud, M., Rapoport, G., Role of BkdR, a transcriptional activator of the SigL-dependent isoleucine and valine degradation pathway in Bacillus subtilis (1999) J Bacteriol, 181, pp. 2059-2066 
504 |a English, J., Vary, P., Isolation of recombination-defective and UV-sensitive mutants of Bacillus megaterium (1986) J Bacteriol, 165, pp. 155-160 
504 |a Jones, D., Woods, D., Acetone-butanol fermentation revisited (1986) Microbiol Rev, 50, pp. 484-524 
504 |a Kunst, F., The complete genome sequence of the Gram-positive bacterium Bacillus subtilis (1997) Nature, 390, pp. 249-256 
504 |a Lach, D.A., Sharma, V.K., Vary, P.S., Isolation and characterization of a unique division mutant of Bacillus megaterium (1990) J Gen Microbiol, 3, pp. 545-553 
504 |a Liu, S.-J., Steinbüchel, A., A novel genetically engineered pathway for synthesis of poly (hydroxyalkanoic acids) in Escherichia coli (2000) Appl Environ Microbiol, 66, pp. 739-743 
504 |a López, N., Floccari, M., García, A., Steinbüchel, A., Méndez, B., Effect of poly (3-hydroxybutyrate) content on the starvation survival of bacteria in natural waters (1995) FEMS Microb Ecol, 16, pp. 95-102 
504 |a Lowry, O., Rosebrough, N., Farr, L., Randall, R., Protein measurement with the Folin phenol reagent (1951) J Biol Chem, 193, pp. 265-275 
504 |a Madison, L.L., Huisman, G.W., Metabolic engineering of poly(3-hydroxyalkanoates): From DNA to plastic (1999) Microbiol Mol Biol Rev, 63, pp. 21-53 
504 |a Pettinari, M.J., Vazquez, G.J., Kruger, N., Vary, P., Steinbüchel, A., Méndez, B.S., Trans activation of the Escherichia coli ato structural genes by a regulatory protein from Bacillus megaterium: Potential use in polyhydroxyalkanoate production (1998) Appl Microbiol Biotechnol, 49, pp. 737-742 
504 |a Strauch, M., Hoch, J., Transition-state regulators: Sentinels of Bacillus subtilis post-exponential gene expression (1993) Molec Microbiol, 7, pp. 337-342 
504 |a Trieu-Cuot, P., Carlier, C., Poyart-Salmeron, C., Courvalin, P., Shuttle vectors containing a multiple cloning site and a lacZ alpha gene for conjugal transfer of DNA from Escherichia coli to gram-positive bacteria (1991) Gene, 102, pp. 99-104 
504 |a Vary, P., Tao, Y.P., Development of genetic methods in Bacillus megaterium (1988) Genetics and Biotechnology of Bacilli, 2, pp. 403-407. , Ganesan AT, Hoch JA (eds). Academic, New York 
504 |a Vazquez, G.J., Pettinari, M.J., Méndez, B.S., Phosphotransbutyrylase expression in Bacillus megaterium (2001) Curr Microbiol, 42, pp. 345-349 
504 |a Walter, K.A., Nair, R.V., Cary, J.N., Bennett, G.N., Papoutsakis, E.T., Sequence and arrangement of two genes of the butyrate-synthesis pathway of Clostridium acetobutylicum ATCC 824 (1993) Gene, 134, pp. 107-111 
520 3 |a Molecular analysis of a genomic region of Bacillus megaterium, a polyhydroxybutyrate (PHB)-producing microorganism, revealed the presence of a gene coding for the enzyme phosphotransbutyrylase (Ptb). Enzyme activity was measured throughout the different growth phases of B. megaterium and was found to correlate with PHB accumulation during the late-exponential growth phase. Ptb expression was repressed by glucose and activated by the branched amino acids isoleucine and valine. Overexpression of ActBm, a σ 54 regulator from B. megaterium whose gene is located upstream from ptb, caused an increase in Ptb activity and PHB accumulation in B. megaterium.  |l eng 
536 |a Detalles de la financiación: Universidad de Buenos Aires 
536 |a Detalles de la financiación: Consejo Nacional de Investigaciones Científicas y Técnicas 
536 |a Detalles de la financiación: Acknowledgements This work was supported by grants from the Universidad de Buenos Aires (UBA) and Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET). GJV had a graduate student fellowship from UBA. BSM is a career investigator from CONICET. 
593 |a Depto. de Quím. Biol., Universidad de Buenos Aires, Fac. de Ciencias Exactas y Naturales, Buenos Aires, Argentina 
593 |a Microbiology Laboratory, Brookhaven National Laboratory, Environmental Sciences Department, Upton, NY, United States 
690 1 0 |a BACILLUS MEGATERIUM 
690 1 0 |a POLYHYDROXYBUTYRATE PHOSPHOTRANSBUTYRYLASE 
690 1 0 |a ENZYME 
690 1 0 |a GLUCOSE 
690 1 0 |a ISOLEUCINE 
690 1 0 |a PHOSPHOTRANSBUTYRYLASE 
690 1 0 |a POLY(3 HYDROXYBUTYRIC ACID) 
690 1 0 |a UNCLASSIFIED DRUG 
690 1 0 |a VALINE 
690 1 0 |a ARTICLE 
690 1 0 |a BACILLUS MEGATERIUM 
690 1 0 |a BACTERIAL GROWTH 
690 1 0 |a CONTROLLED STUDY 
690 1 0 |a CORRELATION ANALYSIS 
690 1 0 |a ENZYME ACTIVITY 
690 1 0 |a GENE LOCATION 
690 1 0 |a GENETIC CODE 
690 1 0 |a GROWTH CURVE 
690 1 0 |a NONHUMAN 
690 1 0 |a PROTEIN EXPRESSION 
690 1 0 |a BACILLUS MEGATERIUM 
690 1 0 |a BACTERIA (MICROORGANISMS) 
700 1 |a Pettinari, M.J. 
700 1 |a Méndez, B.S. 
773 0 |d Springer-Verlag GmbH Co. KG, 2003  |g v. 6  |h pp. 127-129  |k n. 2  |p Int. Microbiol.  |x 11396709  |w (AR-BaUEN)CENRE-1927  |t International Microbiology 
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856 4 0 |u https://hdl.handle.net/20.500.12110/paper_11396709_v6_n2_p127_Vazquez  |y Handle 
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