Assessment of virulence determinants in Yersinia enterocolitica 1A, O:5 strains isolated from chicken carcasses
The virulence potential associated with 42-45 MDa plasmid pYV, of three Yersinia enterocolitica, 1A, O:5, strains isolated previously from chicken carcasses was tested by plasmid extraction, and by four in vitro tests: (1) Autoagglutination; (2) Calcium dependence for growth at 37°C; (3) Congo Red a...
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| Formato: | Capítulo de libro |
| Lenguaje: | Inglés |
| Publicado: |
Springer Netherlands
2003
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| Acceso en línea: | Registro en Scopus DOI Handle Registro en la Biblioteca Digital |
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| Sumario: | The virulence potential associated with 42-45 MDa plasmid pYV, of three Yersinia enterocolitica, 1A, O:5, strains isolated previously from chicken carcasses was tested by plasmid extraction, and by four in vitro tests: (1) Autoagglutination; (2) Calcium dependence for growth at 37°C; (3) Congo Red and Crystal Violet binding; and (4) Resistance to the bactericidal effect of serum. The in vitro tests were negative in all strains tested, but from strain PP131 it was possible to extract a plasmid which was quite similar in size to the wild type pYV, but heavier and not observed in any other isolated strains. These contradictions between the presence of a plasmid of approximately 42-45 MDa and the in vitro tests negative results may suggest that there is no homology between the PP131 plasmid and pYV. From these results we conclude that the plasmid found in PP131 is not pYV and that the three Y. enterocolitica 1A, O:5, strains are non-pathogenic on pYV classical mechanism basis, which do not exclude the possibility of them becoming infectious by a different mechanism, especially in children or immune-depressed individuals. |
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| ISSN: | 09593993 |
| DOI: | 10.1023/A:1025135311746 |