Assessment of virulence determinants in Yersinia enterocolitica 1A, O:5 strains isolated from chicken carcasses

The virulence potential associated with 42-45 MDa plasmid pYV, of three Yersinia enterocolitica, 1A, O:5, strains isolated previously from chicken carcasses was tested by plasmid extraction, and by four in vitro tests: (1) Autoagglutination; (2) Calcium dependence for growth at 37°C; (3) Congo Red a...

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Autor principal: Floccari, M.E
Otros Autores: Carranza, M.M, Parada, J.L
Formato: Capítulo de libro
Lenguaje:Inglés
Publicado: Springer Netherlands 2003
Acceso en línea:Registro en Scopus
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100 1 |a Floccari, M.E. 
245 1 0 |a Assessment of virulence determinants in Yersinia enterocolitica 1A, O:5 strains isolated from chicken carcasses 
260 |b Springer Netherlands  |c 2003 
270 1 0 |m Floccari, M.E.; Area Microbiología, Depto. de Quím. Biol., Ciudad Universitaria (1428), Buenos Aires, Argentina; email: mir@qb.fcen.uba.ar 
506 |2 openaire  |e Política editorial 
504 |a Bleves, S., Cornelis, G.R., How to survive in the host: The Yersinia lesson (2000) Review Microbes and Infection, 2, pp. 1451-1460 
504 |a Bottone, E.J., Yersinia enterocolitica: The charisma continues (1997) Clinical Microbiology Reviews, 10, pp. 257-276 
504 |a Floccari, M.E., Métodos de conservación de cultivos bacterianos. (Bacterial cultures preservation methods) Review (1998) Revista Argentina de Microbiología, 30, pp. 42-51 
504 |a Floccari, M.E., Peso, O.A., Yersinia enterocolitica y especies relacionadas en líquido cloacal de la ciudad de Buenos Aires: Empleo del medio de Schiemann en su aislamiento. (Yersinia enterocolitica and related species in sewage samples from Buenos Aires City: Use of Schiemann's medium for its isolation) (1984) Revista Argentina de Microbiología, 16, pp. 57-66 
504 |a Floccari, M.E., Carranza, M.M., Parada, J.L., Yersinia enterocolitica biogroup 1A serotype 0:5 in chicken carcasses (2000) Journal of Food Protection, 63, pp. 1591-1593 
504 |a Grant, T., Bennett-Wood, V., Robins-Browne, R.M., Identification of virulence-associated characteristics in clinical isolates of Yersinia enterocolitica lacking classical virulence markers (1998) Infection and Immunity, 66, pp. 1113-1120 
504 |a Hoffmann, B., Strauch, E., Gewinner, C., Nattermann, H., Appel, B., Characterization of plasmid regions of foodborne Yersmia enterocolitica biogroup 1A strains hybridizing to the Yersinia enterocolitica virulence plasmid (1998) Systematic and Applied Microbiology, 21, pp. 201-211 
504 |a Irving, R., Holmes, W.M., Hylemon, P.B., Modified plasmid isolation for Clostridium perfringens and Clostridium absonum (1986) Applied and Environmental Microbiology, 52, pp. 197-199 
504 |a Johnson, J.L., Isolation and identification of Yersinia enterocolitica from meat and poultry products (1998) USDA/FSIS Microbiology Laboratory Guidebook 3rd Edn, , Chap. 9, Dey, B.P. & Lattuada, C.P. eds. USA. ISBN 0-16-04-96-02-0 
504 |a Morris, J.G., Prado V., Jr., Ferreccio, C., Robins-Browne, R.M., Bordun, A.M., Cayazzo, M., Kay, B.A., Levine, M.M., Yersinia enterocolitica isolated from two cohorts of young children in Santiago, Chile: Incidence of and lack of correlation between illness and proposed virulence factors (1991) Journal of Clinical Microbiology, 29, pp. 2784-2788 
504 |a Pai, C.H., DeStephano, L., Serum resistance associated with virulence in Yersinia enterocolitica (1982) Infection and Immunity, 35, pp. 605-611 
504 |a Paz, M., Muzio, H., Litardo, L., Vay, C., Famiglieti, A., Santini, P., Characteristics of a Yersinia enteroeolitica 1: O5, Xz strain isolated of human diarrheic feces in Argentina (1998) Revista de Microbiología, 29, pp. 76-78. , Sao Paulo, Brazil 
520 3 |a The virulence potential associated with 42-45 MDa plasmid pYV, of three Yersinia enterocolitica, 1A, O:5, strains isolated previously from chicken carcasses was tested by plasmid extraction, and by four in vitro tests: (1) Autoagglutination; (2) Calcium dependence for growth at 37°C; (3) Congo Red and Crystal Violet binding; and (4) Resistance to the bactericidal effect of serum. The in vitro tests were negative in all strains tested, but from strain PP131 it was possible to extract a plasmid which was quite similar in size to the wild type pYV, but heavier and not observed in any other isolated strains. These contradictions between the presence of a plasmid of approximately 42-45 MDa and the in vitro tests negative results may suggest that there is no homology between the PP131 plasmid and pYV. From these results we conclude that the plasmid found in PP131 is not pYV and that the three Y. enterocolitica 1A, O:5, strains are non-pathogenic on pYV classical mechanism basis, which do not exclude the possibility of them becoming infectious by a different mechanism, especially in children or immune-depressed individuals.  |l eng 
593 |a Area Microbiología, Depto. de Quím. Biol., Ciudad Universitaria (1428), Buenos Aires, Argentina 
593 |a Lab. de Microbiologia de Alimentos, Fac. de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina 
690 1 0 |a CHICKENS CONTAMINATION 
690 1 0 |a PYV VIRULENCE PLASMID PRESENCE TESTS 
690 1 0 |a YERSINIA ENTEROCOLITICA 
690 1 0 |a GALLUS GALLUS 
690 1 0 |a PUFFINUS AURICULARIS 
690 1 0 |a VIOLA (ANGIOSPERM) 
690 1 0 |a YERSINIA 
690 1 0 |a YERSINIA ENTEROCOLITICA 
690 1 0 |a YERSINIA ENTEROCOLITICA 
700 1 |a Carranza, M.M. 
700 1 |a Parada, J.L. 
773 0 |d Springer Netherlands, 2003  |g v. 19  |h pp. 549-550  |k n. 5  |p World J. Microbiol. Biotechnol.  |x 09593993  |t World Journal of Microbiology and Biotechnology 
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