Monitoring by PCR amplification of the polyphosphate kinase gene added to natural water samples.

A fast, simple method for the detection of the Escherichia coli polyphosphate kinase (ppk) gene by means of PCR amplification is described. The method uses filters to recover cells from the samples, which makes it suitable for environmental studies. The detection of the ppk gene was achieved from sa...

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Detalles Bibliográficos
Autor principal: López, N.I
Otros Autores: Pettinari, M.J, Méndez, B.S
Formato: Capítulo de libro
Lenguaje:Inglés
Publicado: 1996
Acceso en línea:Registro en Scopus
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Registro en la Biblioteca Digital
Aporte de:Registro referencial: Solicitar el recurso aquí
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024 7 |2 scopus  |a 2-s2.0-0030342049 
024 7 |2 cas  |a phosphotransferase, 9031-09-8, 9031-44-1; DNA, Bacterial; Phosphotransferases (Phosphate Group Acceptor), EC 2.7.4.-; polyphosphate kinase, EC 2.7.4.1 
040 |a Scopus  |b spa  |c AR-BaUEN  |d AR-BaUEN 
100 1 |a López, N.I. 
245 1 0 |a Monitoring by PCR amplification of the polyphosphate kinase gene added to natural water samples. 
260 |c 1996 
270 1 0 |m López, N.I. 
506 |2 openaire  |e Política editorial 
520 3 |a A fast, simple method for the detection of the Escherichia coli polyphosphate kinase (ppk) gene by means of PCR amplification is described. The method uses filters to recover cells from the samples, which makes it suitable for environmental studies. The detection of the ppk gene was achieved from samples containing 10(2) E. coli cells, either in saline solution or in river water.  |l eng 
593 |a Departamento de Ciencias Biológicas, Facultad de Ciencias Exactas y Naturales, Buenos Aires, Argentina 
690 1 0 |a BACTERIAL DNA 
690 1 0 |a PHOSPHOTRANSFERASE 
690 1 0 |a POLYPHOSPHATE KINASE 
690 1 0 |a ARTICLE 
690 1 0 |a ESCHERICHIA COLI 
690 1 0 |a GENETICS 
690 1 0 |a MICROBIOLOGY 
690 1 0 |a POLYMERASE CHAIN REACTION 
690 1 0 |a DNA, BACTERIAL 
690 1 0 |a ESCHERICHIA COLI 
690 1 0 |a PHOSPHOTRANSFERASES (PHOSPHATE GROUP ACCEPTOR) 
690 1 0 |a POLYMERASE CHAIN REACTION 
690 1 0 |a WATER MICROBIOLOGY 
700 1 |a Pettinari, M.J. 
700 1 |a Méndez, B.S. 
773 0 |d 1996  |g v. 12  |h pp. 557-562  |k n. 4  |p Microbiologia  |x 02134101  |t Microbiología (Madrid, Spain) 
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999 |c 64322